Potato variety improvement through marker assisted selection
This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2021.
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Brac University
2021
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| Առցանց հասանելիություն: | http://hdl.handle.net/10361/15004 |
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10361-150042021-09-14T21:01:30Z Potato variety improvement through marker assisted selection Afzal, Aqief Islam, Aparna Department of Mathematics and Natural Sciences, Brac University Potato LB resistance Inoculums pressure AUDPC rAUDPC Susceptible scale value MAS Late blight (LB) This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2021. Catalogued from PDF version of thesis. Includes bibliographical references (pages 72-77). Late blight (LB), caused by Phytophthora infestans (Mont.) de Bary, is the most destructive for potato. The main objective of this study was to evaluate 72 potato lines, derived from cross products of the recipient cv. ACI Pakri-1 and a LB resistant donor variety, against late blight. The experiment was conducted with three levels of inoculums pressure (i) LB inoculation & no fungicide, (ii) No LB inoculation & no fungicide and (iii) No LB inoculation & fungicide. LB infection was assessed at 10 days interval by scoring the percentage of foliage destruction. Three categories of potato lines were selected considering level of LB infection and tuber appearance - (a) LB resistant - 8 lines, (b) LB tolerant - 14 lines, and (c) LB susceptible - 14 lines. The foliage destruction of selected LB resistant lines was considered to be between 1 to 25%, in LB tolerant lines between 25 to 50 %, and in LB susceptible lines between 51 to 90% at 85 DAP. The recipient ACI Pakri-1 had 100% foliage destruction at 63-65 DAP. On the other hand, lowest foliage destruction 1-2% was recorded in Line 8. In LB resistant lines low AUDPC, rAUDPC and susceptible scale value were found. The highest susceptible scale value 9 was recorded mostly in LB susceptible cv. ACI Pakri-1 and BARI Alu-40; where the range of susceptible scale value 0.1 was calculated in line. However, LB resistant gene Rpi-abpt and Rpi-blb1, amplified using marker R2-F1/R2-R3 and 1521/518 respectively, were identified through marker assisted selection (MAS) in the donor variety and crossed LB resistant line 8, 13, 41, 61, 72 and 54 but absent in recipient ACI Pakri-1. Round tubers, mostly of uniform size, deep to light red and white skin, red and deep eye were found in resistant lines. The highest number of tubers/ hill (20) was recorded in line 8 under natural inoculums pressure, and the highest weight of tuber/hill (460g) was found in line 8 and yield was 38.3 Mt/ha, which was higher than the donor variety (31.5 Mt/ha) and the recipient ACI Pakri-1 (29.0 Mt/ha) under fungicide application treatment. Finally, based on the potentiality against LB and yield performance, Line 8 was registered as potato variety namely ACI LBR Pakri-1 by Seed Wing, Ministry of Agriculture, Bangladesh. Aqief Afzal M. Biotechnology 2021-09-14T06:13:50Z 2021-09-14T06:13:50Z 2021 2021-02 Thesis ID: 17276002 http://hdl.handle.net/10361/15004 en_US Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 77 Pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
en_US |
| topic |
Potato LB resistance Inoculums pressure AUDPC rAUDPC Susceptible scale value MAS Late blight (LB) |
| spellingShingle |
Potato LB resistance Inoculums pressure AUDPC rAUDPC Susceptible scale value MAS Late blight (LB) Afzal, Aqief Potato variety improvement through marker assisted selection |
| description |
This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2021. |
| author2 |
Islam, Aparna |
| author_facet |
Islam, Aparna Afzal, Aqief |
| format |
Thesis |
| author |
Afzal, Aqief |
| author_sort |
Afzal, Aqief |
| title |
Potato variety improvement through marker assisted selection |
| title_short |
Potato variety improvement through marker assisted selection |
| title_full |
Potato variety improvement through marker assisted selection |
| title_fullStr |
Potato variety improvement through marker assisted selection |
| title_full_unstemmed |
Potato variety improvement through marker assisted selection |
| title_sort |
potato variety improvement through marker assisted selection |
| publisher |
Brac University |
| publishDate |
2021 |
| url |
http://hdl.handle.net/10361/15004 |
| work_keys_str_mv |
AT afzalaqief potatovarietyimprovementthroughmarkerassistedselection |
| _version_ |
1814309427750109184 |