Gene cloning and DNA analysis : an introduction /
"The main development in DNA technology since publication of the Seventh Edition of Gene Cloning has been the increased use of gene editing as a tool in both research and biotechnology. The basic methodology for CRISPR editing is now described in Chapter 12 and the applications of the method ar...
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| Format: | Book |
| Language: | English |
| Published: |
Hoboken, NJ :
Wiley-Blackwell,
2021.
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| Edition: | Eighth edition. |
| Subjects: | |
| Classic Catalogue: | View this record in Classic Catalogue |
Table of Contents:
- Why gene cloning and DNA analysis are important
- Vectors for gene cloning : plasmids and bacteriophages
- Purification of DNA from living cells
- Manipulation of purified DNA
- Introduction of DNA into living cells
- Cloning vectors for Escherichia coli
- Cloning vectors for eukaryotes
- How to obtain a clone of a specific gene
- The polymerase chain reaction
- Sequencing genes and genomes
- Studying gene expression and function
- Studying genomes
- Studying transcriptomes and proteomes
- Production of protein from cloned genes
- Gene cloning and DNA analysis in medicine
- Gene cloning and DNA analysis in agriculture
- Gene cloning and DNA analysis in forensic science and archaeology.