Isolation of cellulolytic bacteria from soil sample and production of cellulase and its purification
This thesis report is submitted in partial fulfilment of the requirement for the degree of M.Sc. in Biotechnology, 2018.
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BRAC Univeristy
2018
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10361-98502019-09-30T05:05:43Z Isolation of cellulolytic bacteria from soil sample and production of cellulase and its purification Khanom, Shabrina Hossain, Dr Mahboob Department of Mathematics and Natural Sciences, BRAC University Cellulolytic bacteria This thesis report is submitted in partial fulfilment of the requirement for the degree of M.Sc. in Biotechnology, 2018. Catalogued from PDF version of thesis report. Includes bibliographical references (pages 48-52). The cellulase producing bacteria were isolated from cow dung and characterized by morphological and biochemical analysis. Hence, studies pursue to unfold a novel cellulase that can overcome existing challenges in biorefinery, reduce biofuel production cost, as well as have tremendous applications in industrial processes. Further partial purification of cellulase was carried out by dialysis and ammonium sulphate precipitation. The partially purified enzyme was then purified by gel filtration chromatography using sephadex G-75 matrix and also to determine molecular weight by SDS-PAGE. Bacterial isolate were grown on Carboxy Methyl Cellulose (CMC) agar at various optimum conditions such as parameters like pH, temperature, incubation period prior to examine. In the present study, the result showed that cellulase producing bacteria can grow at optimized condition. The best isolate so far had an extracellular crude enzyme activity of 0.076 U/ml. The use of microorganisms for the production of enzymes offers a promising approach for its large scale production and as a possible food supplement or in the pharmaceutical industry. M. Biotechnology 2018-04-11T05:23:29Z 2018-04-11T05:23:29Z 2018 2018-03 Thesis ID 16376002 http://hdl.handle.net/10361/9850 en BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 55 pages application/pdf BRAC Univeristy |
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Brac University |
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Institutional Repository |
| language |
English |
| topic |
Cellulolytic bacteria |
| spellingShingle |
Cellulolytic bacteria Khanom, Shabrina Isolation of cellulolytic bacteria from soil sample and production of cellulase and its purification |
| description |
This thesis report is submitted in partial fulfilment of the requirement for the degree of M.Sc. in Biotechnology, 2018. |
| author2 |
Hossain, Dr Mahboob |
| author_facet |
Hossain, Dr Mahboob Khanom, Shabrina |
| format |
Thesis |
| author |
Khanom, Shabrina |
| author_sort |
Khanom, Shabrina |
| title |
Isolation of cellulolytic bacteria from soil sample and production of cellulase and its purification |
| title_short |
Isolation of cellulolytic bacteria from soil sample and production of cellulase and its purification |
| title_full |
Isolation of cellulolytic bacteria from soil sample and production of cellulase and its purification |
| title_fullStr |
Isolation of cellulolytic bacteria from soil sample and production of cellulase and its purification |
| title_full_unstemmed |
Isolation of cellulolytic bacteria from soil sample and production of cellulase and its purification |
| title_sort |
isolation of cellulolytic bacteria from soil sample and production of cellulase and its purification |
| publisher |
BRAC Univeristy |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10361/9850 |
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AT khanomshabrina isolationofcellulolyticbacteriafromsoilsampleandproductionofcellulaseanditspurification |
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1814308796653109248 |