Determination of the prevalence and antibiotic susceptibility pattern of the members of Enterobacteriaceae family from the urine samples of UTI suspected patients from a diagnostic center in Dhaka city
This thesis report is submitted in partial fulfilment of the requirement for the degree of B.Sc in Microbiology, 2018.
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BRAC Univeristy
2018
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10361-98122019-09-30T04:43:45Z Determination of the prevalence and antibiotic susceptibility pattern of the members of Enterobacteriaceae family from the urine samples of UTI suspected patients from a diagnostic center in Dhaka city Haque, Syeda Samiha Islam, Namista Department of Mathematics and Natural Sciences, BRAC University Antibiotic This thesis report is submitted in partial fulfilment of the requirement for the degree of B.Sc in Microbiology, 2018. Catalogued from PDF version of thesis report. Includes bibliographical references (pages 57-59). Urinary Tract Infection (UTI) defines a condition in which the urinary tract is infected with a pathogen causing inflammation which is a common, distressing and occasionally life threatening condition. UTI affects people of all ages and both gender. In developing countries, UTI is a common experience in clinical practice. UTI can also lead to bladder infection (cystitis) and kidney. infection (pyelonephritis). Symptoms from a lower urinary tract include pain with urination, frequent urination, and feeling the need to urinate despite having an empty bladder. Symptoms of a kidney infection include fever and flank pain usually in addition to the symptoms of a lower UTI. Rarely the urine may appear bloody. In the very old and the very young, symptoms may be vague or non-specific. Every year millions of people suffer from UTI worldwide, women are mostly affected from this infection. Urinary tract infection is increasing day by day and becoming a life-threatening infection due to its resistance against different antibiotics. Antimicrobial resistance is not only increasing the healthcare costs, but also the severity and death rates from certain infections. In most cases the infectious agents are the members of Enterobacteriaceae family members such as E. coli, Klebsiella spp., Enterobacter spp. and Proteus spp. The aim of this study was to determine the prevalence of the members of Enterobacteriaceae family which are involved in urinary tract infections and the susceptibility of these microorganisms against different antibiotics. 25 urine samples of some UTI suspected male and female patients were collected from a diagnostic center. The samples were plated onto Nutrient agar for total count. Then the isolates were sub-cultured and the selected isolates were grown in MacConkey agar, EMB agar, Hi- Crome agar and Blood agar and Biochemical tests were done for identification of the isolates. About 44 isolates were the members of Enterobacteriaceae family. Klebsiella spp. was predominant pathogen while Enterobacter spp., E. coli and Proteus spp. were also found. Most of the isolates were resistant to Penicillin (90.09%) followed by Erythromycin (84.09%), Ampicillin (75%), Rifampicin (75%) and Cefepime (70.45%), Azithromycin (59.09%), Ciprofloxacin (47.72%) and Tetracycline (31.82%). On the other hand, Streptomycin (2.27%) and Chloramphenicol (9.09%) were found to be effective antibiotics. Considering the above, it is a great matter of concern that the emergence of resistant strains is increasing day by day. However, proper and rational use of antibiotics should be practiced to delay the emergence of the resistant strains. Syeda Samiha Haque B. Science in Microbiology 2018-04-08T05:42:27Z 2018-04-08T05:42:27Z 2018 2018-03 Thesis ID 13126018 http://hdl.handle.net/10361/9812 en BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 67 pages application/pdf BRAC Univeristy |
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Brac University |
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Institutional Repository |
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English |
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Antibiotic |
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Antibiotic Haque, Syeda Samiha Determination of the prevalence and antibiotic susceptibility pattern of the members of Enterobacteriaceae family from the urine samples of UTI suspected patients from a diagnostic center in Dhaka city |
description |
This thesis report is submitted in partial fulfilment of the requirement for the degree of B.Sc in Microbiology, 2018. |
author2 |
Islam, Namista |
author_facet |
Islam, Namista Haque, Syeda Samiha |
format |
Thesis |
author |
Haque, Syeda Samiha |
author_sort |
Haque, Syeda Samiha |
title |
Determination of the prevalence and antibiotic susceptibility pattern of the members of Enterobacteriaceae family from the urine samples of UTI suspected patients from a diagnostic center in Dhaka city |
title_short |
Determination of the prevalence and antibiotic susceptibility pattern of the members of Enterobacteriaceae family from the urine samples of UTI suspected patients from a diagnostic center in Dhaka city |
title_full |
Determination of the prevalence and antibiotic susceptibility pattern of the members of Enterobacteriaceae family from the urine samples of UTI suspected patients from a diagnostic center in Dhaka city |
title_fullStr |
Determination of the prevalence and antibiotic susceptibility pattern of the members of Enterobacteriaceae family from the urine samples of UTI suspected patients from a diagnostic center in Dhaka city |
title_full_unstemmed |
Determination of the prevalence and antibiotic susceptibility pattern of the members of Enterobacteriaceae family from the urine samples of UTI suspected patients from a diagnostic center in Dhaka city |
title_sort |
determination of the prevalence and antibiotic susceptibility pattern of the members of enterobacteriaceae family from the urine samples of uti suspected patients from a diagnostic center in dhaka city |
publisher |
BRAC Univeristy |
publishDate |
2018 |
url |
http://hdl.handle.net/10361/9812 |
work_keys_str_mv |
AT haquesyedasamiha determinationoftheprevalenceandantibioticsusceptibilitypatternofthemembersofenterobacteriaceaefamilyfromtheurinesamplesofutisuspectedpatientsfromadiagnosticcenterindhakacity |
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1814307406362968064 |