Determination of prevalence and antibiotic susceptibility pattern of bacteria isolated from household and restaurant kitchen utensils of Dhaka, Bangladesh
This thesis report is submitted in partial fulfilment of the requirement for the degree of B.Sc in Microbiology, 2018.
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| التنسيق: | أطروحة |
| اللغة: | English |
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BRAC Univeristy
2018
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| الوصول للمادة أونلاين: | http://hdl.handle.net/10361/9802 |
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10361-9802 |
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10361-98022019-09-30T04:33:57Z Determination of prevalence and antibiotic susceptibility pattern of bacteria isolated from household and restaurant kitchen utensils of Dhaka, Bangladesh Anwar, Tashfia Islam, Namista Department of Mathematics and Natural Sciences, BRAC University Kitchen safety Food hygiene Multi-drug resistance This thesis report is submitted in partial fulfilment of the requirement for the degree of B.Sc in Microbiology, 2018. Catalogued from PDF version of thesis report. Includes bibliographical references (pages 62-64). The kitchen is one of the most crucial areas that harbors and transmits infection. This study aimed at isolating, identifying, determining antibiotic susceptibility pattern and investigating antimicrobial activity of dishwashing liquid against the bacterial isolates collected from household and restaurant kitchen utensils (air, knife, spoon and cutting board) of Dhaka, Bangladesh. Sixteen (16) samples collected through sterile swabs were kept for enrichment into nutrient broth at 37ºC for 24 hours and were cultured on various selective media. Identification of bacteria was done through conventional biochemical tests according to Bergey’s Manual of Systematic Bacteriology. Antibiotic susceptibility pattern of all isolates were performed against ten commercial antibiotic discs by using Kirby-Bauer disc diffusion method. Minimum Inhibitory Concentration (MIC) of commercially used dishwasher was also performed using Broth Microdilution method against six selected isolates. A total of about 61 bacterial isolates were identified where Bacillus spp. showed the highest prevalence 18 (29.51%) followed by Vibrio spp. 17 (27.87%), Staphylococcus spp. 13 (21.31%), Klebsiella spp. 6 (9.84%), Shigella spp. 4 (6.56%), Salmonella spp. 2 (3.28%), and E.coli 1 (1.63%). Antibiotic susceptibility pattern of the bacterial isolates showed that almost all of the isolates were resistant to at least two antibiotics. Among the 61 isolates, 72.13% were found resistant to more than two antibiotics and 27.87% were found resistant to two antibiotics. Highest resistance percentage of the isolates were observed to penicillin (83.61%) followed by cefixime (60.65%), amoxicillin and SXT (50.82%), rifampicin (49.18%), cefepime (40.98%), chloramphenicol (18.03%), tetracycline (14.71%), ciprofloxacin (6.56%) and gentamycin (1.64%). Broth Microdilution assay revealed that Shigella spp. showed the highest MIC value (400μl of stock solution) and Staphylococcus spp. showed the lowest MIC value (190μl of stock solution). Besides, Escherichia coli, Salmonella spp., Vibrio spp. and Bacillus spp. showed the MIC values 250μl, 300μl, 300μl and 250μl of stock solution respectively. These results indicate that, household and restaurant kitchens can easily be contaminated with a variety of bacterial contaminants. Findings of this study also indicated the presence of multi-drug resistant bacterial strains in kitchen utensils which can serve as potential source of food-borne diseases. Regular cleaning of kitchen utensils, use of dishwashing liquid and public awareness on personal hygiene can help minimize the spread of food-borne diseases from kitchen utensils. Tashfia Anwar B. Science in Microbiology 2018-04-04T06:25:18Z 2018-04-04T06:25:18Z 2018 2018-03 Thesis ID 12226002 http://hdl.handle.net/10361/9802 en BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 75 pages application/pdf BRAC Univeristy |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Kitchen safety Food hygiene Multi-drug resistance |
| spellingShingle |
Kitchen safety Food hygiene Multi-drug resistance Anwar, Tashfia Determination of prevalence and antibiotic susceptibility pattern of bacteria isolated from household and restaurant kitchen utensils of Dhaka, Bangladesh |
| description |
This thesis report is submitted in partial fulfilment of the requirement for the degree of B.Sc in Microbiology, 2018. |
| author2 |
Islam, Namista |
| author_facet |
Islam, Namista Anwar, Tashfia |
| format |
Thesis |
| author |
Anwar, Tashfia |
| author_sort |
Anwar, Tashfia |
| title |
Determination of prevalence and antibiotic susceptibility pattern of bacteria isolated from household and restaurant kitchen utensils of Dhaka, Bangladesh |
| title_short |
Determination of prevalence and antibiotic susceptibility pattern of bacteria isolated from household and restaurant kitchen utensils of Dhaka, Bangladesh |
| title_full |
Determination of prevalence and antibiotic susceptibility pattern of bacteria isolated from household and restaurant kitchen utensils of Dhaka, Bangladesh |
| title_fullStr |
Determination of prevalence and antibiotic susceptibility pattern of bacteria isolated from household and restaurant kitchen utensils of Dhaka, Bangladesh |
| title_full_unstemmed |
Determination of prevalence and antibiotic susceptibility pattern of bacteria isolated from household and restaurant kitchen utensils of Dhaka, Bangladesh |
| title_sort |
determination of prevalence and antibiotic susceptibility pattern of bacteria isolated from household and restaurant kitchen utensils of dhaka, bangladesh |
| publisher |
BRAC Univeristy |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10361/9802 |
| work_keys_str_mv |
AT anwartashfia determinationofprevalenceandantibioticsusceptibilitypatternofbacteriaisolatedfromhouseholdandrestaurantkitchenutensilsofdhakabangladesh |
| _version_ |
1814308945903222784 |