Comparative study of the presence of stx1 and stx2 genes from clinical and environmental samples of Shigella
This thesis report is submitted in partial fulfillment of the requirement for the degree of B.Sc in Microbiology, 2017.
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| Định dạng: | Luận văn |
| Ngôn ngữ: | English |
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BRAC University
2018
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| Truy cập trực tuyến: | http://hdl.handle.net/10361/9465 |
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10361-94652019-09-30T03:20:10Z Comparative study of the presence of stx1 and stx2 genes from clinical and environmental samples of Shigella Annur, Rocksher Mosharraf, Fahareen-Binta Department of Mathematics and Natural Sciences, BRAC University Shigellosis Shigella spp stx1 stx2 PCR technique This thesis report is submitted in partial fulfillment of the requirement for the degree of B.Sc in Microbiology, 2017. Cataloged from PDF version of thesis report. Includes bibliographical references (pages 40). Shigellosis, a disease caused by Shigella spp. occur because of the presence of stx1 and stx2 genes. Shigellaspp. Sometimes escapes detection by the traditional culture methods. Bacterial isolates were chosen on the basis of their structural, morphological and biochemical character. To get the most efficient result along with traditional method PCR technique was used. Antibiotic sensitivity testing was done to see the resistance of the isolates against Amoxicillin, Ampicillin, Tetracyclin, Chloramphenicol, Norfloxacinantibiotic.To detect stx1, Ka1F and Ka1R primers were used which yielded 348 bp fragment of stx1 gene. For stx2 gene Ka2F and Ka2R primers were used to get 584 bp fragment of stx2 gene. The thermal profile for 35 cycles were set using the following parameters 94°C for 10 minutes,94°C for 1 minute,55°C for 1 minute,72°C for 1 minute and 72°C for 7 minutes. This study was conducted to see the presence of Shigella spp. on the environment. Also, to find out if the environmental Shigella spp. has stx1 and stx2 gene required to cause disease. Rocksher Annur B. Mircobiology 2018-02-14T09:32:07Z 2018-02-14T09:32:07Z 2017 2017-03 Thesis ID 13126014 http://hdl.handle.net/10361/9465 en BRAC University thesis reports are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 40 pages application/pdf BRAC University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Shigellosis Shigella spp stx1 stx2 PCR technique |
| spellingShingle |
Shigellosis Shigella spp stx1 stx2 PCR technique Annur, Rocksher Comparative study of the presence of stx1 and stx2 genes from clinical and environmental samples of Shigella |
| description |
This thesis report is submitted in partial fulfillment of the requirement for the degree of B.Sc in Microbiology, 2017. |
| author2 |
Mosharraf, Fahareen-Binta |
| author_facet |
Mosharraf, Fahareen-Binta Annur, Rocksher |
| format |
Thesis |
| author |
Annur, Rocksher |
| author_sort |
Annur, Rocksher |
| title |
Comparative study of the presence of stx1 and stx2 genes from clinical and environmental samples of Shigella |
| title_short |
Comparative study of the presence of stx1 and stx2 genes from clinical and environmental samples of Shigella |
| title_full |
Comparative study of the presence of stx1 and stx2 genes from clinical and environmental samples of Shigella |
| title_fullStr |
Comparative study of the presence of stx1 and stx2 genes from clinical and environmental samples of Shigella |
| title_full_unstemmed |
Comparative study of the presence of stx1 and stx2 genes from clinical and environmental samples of Shigella |
| title_sort |
comparative study of the presence of stx1 and stx2 genes from clinical and environmental samples of shigella |
| publisher |
BRAC University |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10361/9465 |
| work_keys_str_mv |
AT annurrocksher comparativestudyofthepresenceofstx1andstx2genesfromclinicalandenvironmentalsamplesofshigella |
| _version_ |
1814308448946356224 |