Phytochemical screening and comparative study of antibacterial assays of carum roxburghianum and trigonella foenum-graecum obtained grom plant seed
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2016.
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2018
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10361-94592019-09-30T03:33:58Z Phytochemical screening and comparative study of antibacterial assays of carum roxburghianum and trigonella foenum-graecum obtained grom plant seed Amin, Fatima Islam, Zubaida Marufee Department of Mathematics and Natural Sciences, BRAC University Phytochemical screening Assays Carum roxburghianum Plant seed Trigonella foenum-graecum This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2016. Cataloged from PDF version of thesis report. Includes bibliographical references (pages 61-66). To control the infections and microbial food spoilage the application of chemical or synthetic antimicrobial agents is one of the ancient techniques for which an enormous number of microbes are escalating resistance to synthetic antibiotics. Therefore, there has been immense concern in the development of efficient and nontoxic antimicrobial agents from natural sources, such as extracts of plants for skirmishing comprised health condition and food preservation. In this current research work, the crude extracts of Carum roxburghianum (radhuni) and Trigonella foenum-graecum (fenugreek) were collected using two solvents namely Ethanol and Methanol. Antibacterial effects of ethanol and methanol extracts taken of second, fifth and seventh days and observed on selected eleven bacteria where extracts of different days had different effects on six bacteria for radhuni showing positive result against Shigella flexinera, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus and Streptococcus pneumonae and fenugreek showed positive result only for Bacillus cereus. The ethanol extracts of both seeds showed the greatest positive result where Radhuni from the fifth day against Bacillus subtilis and Fenugreek from the seventh day against Bacillus cereus. Moreover, comparing the antibacterial activities between radhuni and fenugreek seed extract, the zone of inhibition was greater for radhuni than fenugreek against Bacillus cereus. The activity index of ethanol extract of day five was the highest against Shigella flexineri indicating high sensitivity to the extract. Phytochemical assay such as tests for alkaloids, terpenoids, tannins, saponins, steroids, phenolic compounds, flavonoids and cardiac glycosides was performed on both seed extracts to identify the secondary metabolites present in them. The results achieved from this research can act as a milestone in exploring the new arena of antimicrobial properties of both extracts chiefly for radhuni extracts. This study could be the starting of discovering some novel and less expensive microbial agent against various bacterial species. Fatima Amin B. Biotechnology 2018-02-14T05:11:10Z 2018-02-14T05:11:10Z 2016 2016-11 Thesis ID 11136009 http://hdl.handle.net/10361/9459 en BRAC University thesis reports are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 68 pages application/pdf BRAC University |
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Brac University |
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Institutional Repository |
language |
English |
topic |
Phytochemical screening Assays Carum roxburghianum Plant seed Trigonella foenum-graecum |
spellingShingle |
Phytochemical screening Assays Carum roxburghianum Plant seed Trigonella foenum-graecum Amin, Fatima Phytochemical screening and comparative study of antibacterial assays of carum roxburghianum and trigonella foenum-graecum obtained grom plant seed |
description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2016. |
author2 |
Islam, Zubaida Marufee |
author_facet |
Islam, Zubaida Marufee Amin, Fatima |
format |
Thesis |
author |
Amin, Fatima |
author_sort |
Amin, Fatima |
title |
Phytochemical screening and comparative study of antibacterial assays of carum roxburghianum and trigonella foenum-graecum obtained grom plant seed |
title_short |
Phytochemical screening and comparative study of antibacterial assays of carum roxburghianum and trigonella foenum-graecum obtained grom plant seed |
title_full |
Phytochemical screening and comparative study of antibacterial assays of carum roxburghianum and trigonella foenum-graecum obtained grom plant seed |
title_fullStr |
Phytochemical screening and comparative study of antibacterial assays of carum roxburghianum and trigonella foenum-graecum obtained grom plant seed |
title_full_unstemmed |
Phytochemical screening and comparative study of antibacterial assays of carum roxburghianum and trigonella foenum-graecum obtained grom plant seed |
title_sort |
phytochemical screening and comparative study of antibacterial assays of carum roxburghianum and trigonella foenum-graecum obtained grom plant seed |
publisher |
BRAC University |
publishDate |
2018 |
url |
http://hdl.handle.net/10361/9459 |
work_keys_str_mv |
AT aminfatima phytochemicalscreeningandcomparativestudyofantibacterialassaysofcarumroxburghianumandtrigonellafoenumgraecumobtainedgromplantseed |
_version_ |
1814309691156594688 |