Isolation, identification and characterization of Rhizobium from hyacinth bean (Lablab niger) root nodule and study its effect on plant growth and nitrogen fixation ability
This thesis report is submitted in partial fulfillment of the requirement for the degree of B.Sc in Microbiology, 2017.
| Yazar: | |
|---|---|
| Diğer Yazarlar: | |
| Materyal Türü: | Tez |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
BRAC University
2018
|
| Konular: | |
| Online Erişim: | http://hdl.handle.net/10361/9166 |
| id |
10361-9166 |
|---|---|
| record_format |
dspace |
| spelling |
10361-91662019-09-30T03:18:01Z Isolation, identification and characterization of Rhizobium from hyacinth bean (Lablab niger) root nodule and study its effect on plant growth and nitrogen fixation ability Yshita, Khadiza Nasrin Hossain, Dr. M Mahboob Department of Mathematics and Natural Sciences, BRAC University Rhizobium Nitrogen This thesis report is submitted in partial fulfillment of the requirement for the degree of B.Sc in Microbiology, 2017. Cataloged from PDF version of thesis report. Includes bibliographical references (pages 29-35). Symbiotic systems for biological nitrogen fixation (BNF) in agriculture are most promising. Accordingly, the present study was conducted for the isolation, identification, and characterization of nitrogen-fixing bacterial strains Rhizobium from the legume plant hyacinth bean which grows widely in almost every area of Bangladesh. Nitrogen fixation is the reduction of N2 (atmospheric nitrogen) to NH3 (ammonia) which is made possible by the enzyme nitrogenase. Strains of root-nodulating bacteria were isolated from root a nodule of Lablab niger known as hyacinth bean. After confirmation test with yeast mannitol agar (YMA), isolates were collected for morphological and biochemical characterization. For nitrogen fixation test, healthy seeds of hyacinth bean were used for pot trials. Previously sterile soil of one pot (marked with green colour) was inoculated with Rhizobium sp. isolated and identified in the present study and another pot (marked in white colour) was not inoculated with bacteria. Four seeds were sown in each pot and two seeds were germinated on green pot whereas one of four seeds was germinated on white pot. After forty-five days of seed sowing, the plant grown in a pot with Rhizobium showed nodulation. Plants from the same pot were healthier than the plant of other pot (white). Khadiza Nasrin Yshita B. Mircobiology 2018-01-24T08:59:38Z 2018-01-24T08:59:38Z 2016 2016-12-01 Thesis ID 10326006 http://hdl.handle.net/10361/9166 en BRAC University thesis reports are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 36 pages application/pdf BRAC University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Rhizobium Nitrogen |
| spellingShingle |
Rhizobium Nitrogen Yshita, Khadiza Nasrin Isolation, identification and characterization of Rhizobium from hyacinth bean (Lablab niger) root nodule and study its effect on plant growth and nitrogen fixation ability |
| description |
This thesis report is submitted in partial fulfillment of the requirement for the degree of B.Sc in Microbiology, 2017. |
| author2 |
Hossain, Dr. M Mahboob |
| author_facet |
Hossain, Dr. M Mahboob Yshita, Khadiza Nasrin |
| format |
Thesis |
| author |
Yshita, Khadiza Nasrin |
| author_sort |
Yshita, Khadiza Nasrin |
| title |
Isolation, identification and characterization of Rhizobium from hyacinth bean (Lablab niger) root nodule and study its effect on plant growth and nitrogen fixation ability |
| title_short |
Isolation, identification and characterization of Rhizobium from hyacinth bean (Lablab niger) root nodule and study its effect on plant growth and nitrogen fixation ability |
| title_full |
Isolation, identification and characterization of Rhizobium from hyacinth bean (Lablab niger) root nodule and study its effect on plant growth and nitrogen fixation ability |
| title_fullStr |
Isolation, identification and characterization of Rhizobium from hyacinth bean (Lablab niger) root nodule and study its effect on plant growth and nitrogen fixation ability |
| title_full_unstemmed |
Isolation, identification and characterization of Rhizobium from hyacinth bean (Lablab niger) root nodule and study its effect on plant growth and nitrogen fixation ability |
| title_sort |
isolation, identification and characterization of rhizobium from hyacinth bean (lablab niger) root nodule and study its effect on plant growth and nitrogen fixation ability |
| publisher |
BRAC University |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10361/9166 |
| work_keys_str_mv |
AT yshitakhadizanasrin isolationidentificationandcharacterizationofrhizobiumfromhyacinthbeanlablabnigerrootnoduleandstudyitseffectonplantgrowthandnitrogenfixationability |
| _version_ |
1814306909882155008 |