Study on the antibacterial activity of liquid hand-wash containing triclosan against clinical samples

This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2017.

Detalles Bibliográficos
Autor principal:	Mustafa, Fuad
Otros Autores:	Jahan, Nazneen
Formato:	Tesis
Lenguaje:	English
Publicado:	BRAC University 2018
Materias:	Antibacterial agents
Acceso en línea:	http://hdl.handle.net/10361/9165

id	10361-9165
record_format	dspace
spelling	10361-91652019-09-30T03:19:35Z Study on the antibacterial activity of liquid hand-wash containing triclosan against clinical samples Mustafa, Fuad Jahan, Nazneen Khan, Troshporsha Tasnim Department of Mathematics and Natural Sciences, BRAC University Antibacterial agents This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2017. Cataloged from PDF version of thesis report. Includes bibliographical references (page 38-46). In recent times the use of antibacterial hand washes has been heavily promoted to a healthconscious public. The purpose of this study was to investigate in vitro antimicrobial activity of liquid hand soap containing triclosan against microorganisms. Eight clinical isolates were used in this studyand they were Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Salmonella typhi, Proteus vulgaris, Shigella flexneri, Bacillus subtilis and Staphylococcus aureus. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was determined for the microorganisms through Broth Microdilution method. Kirby-Bauer disc diffusion test of the same organisms was done using the following antibiotics: Erythromycin (15g), Chloramphenicol (30 g), Ciprofloxacin (5g), Gentamicin (10 g), Kanamycin (10 g), Nalidixic acid (30 g), Penicillin (10g), Streptomycin (10g), Tetracycline (30 g). In this study, Salmonella typhi showed the highest MIC value (3.0 ml of stock solution) and Bacillus subtilis, Staphylococcus aureus had shown the lowest MIC value (0.6 ml of stock solution). Besides, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaris, Shigella flexneri had shown the values 2.6 ml, 2.4 ml, 2.0 ml, 1.0 ml, 0.8 ml of stock solution respectively. All of the clinical isolates taken for the study had shown resistant characteristics against at least six tested antibiotics and three isolates were resistant to all the nine antibiotics. It is possible that antibacterial liquid hand-washes have the antibacterial agents (e.g. Triclosan) that can either kill or inhibit the bacterial cells. It might be possible that some bacterial strain can develop resistant properties which leads to their survival even at higher concentration of soaps and antibiotics. Fuad Mustafa B. Biotechnology 2018-01-24T08:37:19Z 2018-01-24T08:37:19Z 2017 2017-10 Thesis ID 13336010 http://hdl.handle.net/10361/9165 en BRAC University thesis reports are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 48 pages application/pdf BRAC University
institution	Brac University
collection	Institutional Repository
language	English
topic	Antibacterial agents
spellingShingle	Antibacterial agents Mustafa, Fuad Study on the antibacterial activity of liquid hand-wash containing triclosan against clinical samples
description	This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2017.
author2	Jahan, Nazneen
author_facet	Jahan, Nazneen Mustafa, Fuad
format	Thesis
author	Mustafa, Fuad
author_sort	Mustafa, Fuad
title	Study on the antibacterial activity of liquid hand-wash containing triclosan against clinical samples
title_short	Study on the antibacterial activity of liquid hand-wash containing triclosan against clinical samples
title_full	Study on the antibacterial activity of liquid hand-wash containing triclosan against clinical samples
title_fullStr	Study on the antibacterial activity of liquid hand-wash containing triclosan against clinical samples
title_full_unstemmed	Study on the antibacterial activity of liquid hand-wash containing triclosan against clinical samples
title_sort	study on the antibacterial activity of liquid hand-wash containing triclosan against clinical samples
publisher	BRAC University
publishDate	2018
url	http://hdl.handle.net/10361/9165
work_keys_str_mv	AT mustafafuad studyontheantibacterialactivityofliquidhandwashcontainingtriclosanagainstclinicalsamples
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Study on the antibacterial activity of liquid hand-wash containing triclosan against clinical samples

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