Isolation of Lactoferrin from raw and commercial milk samples followed by the observation of antimicrobial activity against Pathogens

This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2017.

Détails bibliographiques
Auteur principal:	Mimmi, Syeda Fahria Hoque
Autres auteurs:	Khan, Kashmery
Format:	Thèse
Langue:	English
Publié:	BRAC University 2018
Sujets:	Lactoferrin Pathogens
Accès en ligne:	http://hdl.handle.net/10361/9147

id	10361-9147
record_format	dspace
spelling	10361-91472019-09-30T03:08:00Z Isolation of Lactoferrin from raw and commercial milk samples followed by the observation of antimicrobial activity against Pathogens Mimmi, Syeda Fahria Hoque Khan, Kashmery Department of Mathematics and Natural Sciences, BRAC University Lactoferrin Pathogens This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2017. Cataloged from PDF version of thesis. Includes bibliographical references (page 52-55). Lf is a multifunctional glycoprotein with 80 kDa size found in the milk of most mammals. In addition to its well-known role of binding iron, Lf carries many important biological functions, including the promotion of cell proliferation and differentiation, and as an anti-bacterial, anti-viral, and anti-parasitic protein. Milk is the highest quality source of well-balanced nutrients and also displays a range of biological activities that affects digestion, metabolic responses to absorbed nutrients, growth & development of specific organs, and resistance to disease. The purpose of this study is to isolate Lf from different milk samples like raw and commercial milk samples and observe the antimicrobial activity of them. Along with this, the quantity of Lf was differentiated between raw and commercial milk samples. Firstly, Lf was extracted from both raw and commercial milk by acid-base adjustments. Then the concentration of isolated Lf protein was measured by NanoDrop technology and the quantities were compared between the raw and commercial ones. Finally, Lf was identified by SDS-PAGE where 5 bands of Lf were observed among eleven samples. After that, all the samples were tested for their antimicrobial activity against 18 pathogens collected from laboratory stocks. Well diffusion method was used to conduct this test. Lf containing samples showed ZOI against some of the pathogens but some other samples those do not contain Lf also gave ZOI against some of the pathogens. The reason could be, though they do not contain Lf but they contain such proteins that can reduce the microbial growth. All these results are collected and discussed in this study. The raw milk contain higher quantity of Lf than the commercial milk and Lf showed effective results against pathogens that can cause infectious disease in human body. This study suggested that Lf can be used as the potential alternative of antibiotics for many diseases and also can be used to reduce microbial deterioration in food and feed industry. Syeda Fahria Hoque Mimmi B. Biotechnology 2018-01-23T10:17:37Z 2018-01-23T10:17:37Z 2017 2017-08 Thesis ID 13136015 http://hdl.handle.net/10361/9147 en BRAC University thesis reports are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 60 pages application/pdf BRAC University
institution	Brac University
collection	Institutional Repository
language	English
topic	Lactoferrin Pathogens
spellingShingle	Lactoferrin Pathogens Mimmi, Syeda Fahria Hoque Isolation of Lactoferrin from raw and commercial milk samples followed by the observation of antimicrobial activity against Pathogens
description	This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2017.
author2	Khan, Kashmery
author_facet	Khan, Kashmery Mimmi, Syeda Fahria Hoque
format	Thesis
author	Mimmi, Syeda Fahria Hoque
author_sort	Mimmi, Syeda Fahria Hoque
title	Isolation of Lactoferrin from raw and commercial milk samples followed by the observation of antimicrobial activity against Pathogens
title_short	Isolation of Lactoferrin from raw and commercial milk samples followed by the observation of antimicrobial activity against Pathogens
title_full	Isolation of Lactoferrin from raw and commercial milk samples followed by the observation of antimicrobial activity against Pathogens
title_fullStr	Isolation of Lactoferrin from raw and commercial milk samples followed by the observation of antimicrobial activity against Pathogens
title_full_unstemmed	Isolation of Lactoferrin from raw and commercial milk samples followed by the observation of antimicrobial activity against Pathogens
title_sort	isolation of lactoferrin from raw and commercial milk samples followed by the observation of antimicrobial activity against pathogens
publisher	BRAC University
publishDate	2018
url	http://hdl.handle.net/10361/9147
work_keys_str_mv	AT mimmisyedafahriahoque isolationoflactoferrinfromrawandcommercialmilksamplesfollowedbytheobservationofantimicrobialactivityagainstpathogens
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Isolation of Lactoferrin from raw and commercial milk samples followed by the observation of antimicrobial activity against Pathogens

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