Combination of several therapeutic drugs with tetracycline to combat highly drug resistant urinary tract infection pathogens
This thesis report is submitted in partial fulfillment of the requirement for the degree of B.Sc in Microbiology, 2017.
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| שפה: | English |
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BRAC University
2018
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| גישה מקוונת: | http://hdl.handle.net/10361/9095 |
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10361-9095 |
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10361-90952019-09-30T03:19:08Z Combination of several therapeutic drugs with tetracycline to combat highly drug resistant urinary tract infection pathogens Aftab, Ishrat Binte Dr. M. Mahboob Hossain Department of Mathematics and Natural Sciences, BRAC University Therapeutic drugs Urinary tract infection Tetracycline UTI pathogens This thesis report is submitted in partial fulfillment of the requirement for the degree of B.Sc in Microbiology, 2017. Cataloged from PDF version of thesis report. Includes bibliographical references (pages 45-49). Urinary tract infection (UTI) is one of the most frequent bacterial infections worldwide. Presently, the most crucial factor of treating UTI is antibiotic resistance. The overutilization of antibiotics has become the greatest pitfall of modern public health development process. This study was aimed to find out a combination of drug with tetracycline to combat UTI pathogens- Klebsiella pneumoniae & Pseudomonas aeruginosa. A number of 90 samples were collected, of them 47 (52.22%) were K. pneumoniae and 43 (47.78%) were P. aeruginosa. They were further categorized into multidrug resistant (MDR), extensively drug resistant (XDR) and pan drug resistant (PDR) pathogens. A total of 11 antibiotics were trialed in combination with tetracycline against these two pathogens. Antibiotic susceptibility test (disk diffusion method) was done for primary screening and minimum inhibitory concentration (MIC) test was done for more accurate results. A fractional inhibitory concentration (FIC) index was calculated to evaluate the effect of combined drugs. A value of <0.5 indicates synergistic effect and more than 4 shows antagonism. Among all the combined drug trials, tetracycline-amoxicillin with clavulanic acid and tetracycline-chloramphenicol showed great efficiency. Moreover, tetracycline-amoxicillin with clavulanic acid and tetracycline-chloramphenicol both combinations had FIC index value of 0.3278 and 0.3785 respectively, indicating that both of them had synergistic effect. A further extension of validation by in vivo animal model study needs to be performed to know whether the combination is suitable for human. Ishrat Binte Aftab B. Mircobiology 2018-01-17T06:29:39Z 2018-01-17T06:29:39Z 2017 2017-12 Thesis ID 14126010 http://hdl.handle.net/10361/9095 en BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 51 pages application/pdf BRAC University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Therapeutic drugs Urinary tract infection Tetracycline UTI pathogens |
| spellingShingle |
Therapeutic drugs Urinary tract infection Tetracycline UTI pathogens Aftab, Ishrat Binte Combination of several therapeutic drugs with tetracycline to combat highly drug resistant urinary tract infection pathogens |
| description |
This thesis report is submitted in partial fulfillment of the requirement for the degree of B.Sc in Microbiology, 2017. |
| author2 |
Dr. M. Mahboob Hossain |
| author_facet |
Dr. M. Mahboob Hossain Aftab, Ishrat Binte |
| format |
Thesis |
| author |
Aftab, Ishrat Binte |
| author_sort |
Aftab, Ishrat Binte |
| title |
Combination of several therapeutic drugs with tetracycline to combat highly drug resistant urinary tract infection pathogens |
| title_short |
Combination of several therapeutic drugs with tetracycline to combat highly drug resistant urinary tract infection pathogens |
| title_full |
Combination of several therapeutic drugs with tetracycline to combat highly drug resistant urinary tract infection pathogens |
| title_fullStr |
Combination of several therapeutic drugs with tetracycline to combat highly drug resistant urinary tract infection pathogens |
| title_full_unstemmed |
Combination of several therapeutic drugs with tetracycline to combat highly drug resistant urinary tract infection pathogens |
| title_sort |
combination of several therapeutic drugs with tetracycline to combat highly drug resistant urinary tract infection pathogens |
| publisher |
BRAC University |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10361/9095 |
| work_keys_str_mv |
AT aftabishratbinte combinationofseveraltherapeuticdrugswithtetracyclinetocombathighlydrugresistanturinarytractinfectionpathogens |
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1814308447303237632 |