Investigation of in-vitro antioxidant potential in crotalaria verrucosa along with identification and quantification of its polyphenolic compounds
This project report is submitted in a partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2016
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BRAC University
2017
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| Linkit: | http://hdl.handle.net/10361/7892 |
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10361-78922019-09-30T03:23:33Z Investigation of in-vitro antioxidant potential in crotalaria verrucosa along with identification and quantification of its polyphenolic compounds Ahmed, Zainab Syed Akter, Dr. Raushanara Department of Pharmacy, BRAC University In-vitro antioxidant Crotalaria verrucosa Polyphenolic compounds This project report is submitted in a partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2016 Cataloged from PDF version of project report. Includes bibliographical references (page 84-91). Crotalaria verrucosa (Fabaceae) is a local medicinal plant, native to Chittagong, Khulna, Rajshahi and Sylhet, in Bangladesh. Traditionally, C. verrucosa is used for the treatment of folkloric remedies namely, scabies, heart complaints as well as in the treatment of throat and oral diseases which may be extrapolated to its antioxidant potential as deduced from this study. In this study, the preliminary phytochemical screening of methanolic leaf extract of C. verrucosa showed the presence of flavonoids, phenolic compounds, alkaloids, tannin, steroids and glycosides. Various in-vitro antioxidant studies were performed to determine its antioxidant potential. The extract showed moderate antioxidant activity in DPPH free radical scavenging assay where ascorbic acid was used as the reference standard. Also, using the DPPH free radical scavenging assay, the IC50 values of Crotalaria verrucosa and ascorbic acid was found to be 533.738ug/mL and 154.916ug/mL, respectively. Whilst the total phenolic content was found to be 152.180mg/g of gallic acid equivalent (GAE), the total flavonoid content was found to be much higher, 184.510mg/g of quercetin equivalent (QE). The total antioxidant capacity was determined to be 32.342mg of ascorbic acid equivalent (AAE) for a concentration of 1200ug/mL of methanolic extract of C. verrucosa. In addition to the determination of in vitro antioxidant potential, HPLC coupled with a diode-array-detector was used to identify and quantify the polyphenolic compounds present in the methanolic crude extract of C. verrucosa. Amongst the polyphenolic compounds, the most notable of them were identified as gallic acid, (+)-catechin hydrate, vanillic acid, caffeic acid, syringic acid, (–)-epicatechin and vanillin were identified present in concentrations of 19.53, 5.08, 5.97, 6.22, 1.09, 7.16 and 8.05mg/100g of dried extract. Amongst the polyphenolic compounds quantified, gallic acid was most predominantly present (19.53mg/100g of dried extract). Although vanillic acid is a catechin-metabolite; (+)-catechin hydrate and (–)-epicatechin are flavonoids and gallic acid, syringic acid, caffeic acid and vanillin are polyphenolic compounds, all of these compounds possess established antioxidant activities. Zainab Syed Ahmed B. Pharmacy 2017-03-13T05:29:45Z 2017-03-13T05:29:45Z 2016 2016-02 Project report ID 12146002 http://hdl.handle.net/10361/7892 en BRAC University project report are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 91 pages application/pdf BRAC University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
In-vitro antioxidant Crotalaria verrucosa Polyphenolic compounds |
| spellingShingle |
In-vitro antioxidant Crotalaria verrucosa Polyphenolic compounds Ahmed, Zainab Syed Investigation of in-vitro antioxidant potential in crotalaria verrucosa along with identification and quantification of its polyphenolic compounds |
| description |
This project report is submitted in a partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2016 |
| author2 |
Akter, Dr. Raushanara |
| author_facet |
Akter, Dr. Raushanara Ahmed, Zainab Syed |
| format |
Project report |
| author |
Ahmed, Zainab Syed |
| author_sort |
Ahmed, Zainab Syed |
| title |
Investigation of in-vitro antioxidant potential in crotalaria verrucosa along with identification and quantification of its polyphenolic compounds |
| title_short |
Investigation of in-vitro antioxidant potential in crotalaria verrucosa along with identification and quantification of its polyphenolic compounds |
| title_full |
Investigation of in-vitro antioxidant potential in crotalaria verrucosa along with identification and quantification of its polyphenolic compounds |
| title_fullStr |
Investigation of in-vitro antioxidant potential in crotalaria verrucosa along with identification and quantification of its polyphenolic compounds |
| title_full_unstemmed |
Investigation of in-vitro antioxidant potential in crotalaria verrucosa along with identification and quantification of its polyphenolic compounds |
| title_sort |
investigation of in-vitro antioxidant potential in crotalaria verrucosa along with identification and quantification of its polyphenolic compounds |
| publisher |
BRAC University |
| publishDate |
2017 |
| url |
http://hdl.handle.net/10361/7892 |
| work_keys_str_mv |
AT ahmedzainabsyed investigationofinvitroantioxidantpotentialincrotalariaverrucosaalongwithidentificationandquantificationofitspolyphenoliccompounds |
| _version_ |
1814307327032950784 |