In Vitro regeneration and rapid multiplication of two orchid varieties of Dendrobium bensoniae and Dendrobium aphyllum
A Dissertation Submitted in Partial Fulfillment of the Requirements’ for the Degree of Master of Science in Biotechnology 2016.
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BRAC University
2017
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| 在线阅读: | http://hdl.handle.net/10361/7797 |
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10361-77972019-09-30T02:59:05Z In Vitro regeneration and rapid multiplication of two orchid varieties of Dendrobium bensoniae and Dendrobium aphyllum Riva, Sahida Siraj Islam, Aparna Hoque, Ekramul Department of Mathematics and Natural Sciences, BRAC University Dendrobium bensoniae Dendrobium aphyllum A Dissertation Submitted in Partial Fulfillment of the Requirements’ for the Degree of Master of Science in Biotechnology 2016. Cataloged from PDF version of thesis report. Includes bibliographical references (page 63-67). The effect of different concentrations of BA and IBA on in vitro plant regeneration was studied in both varieties of Dendrobium bensoniae and Dendrobium aphyllum. The composition of the culture medium using different hormone along or in combination affected the induction, regeneration and multiplication of both varieties of Dendrobium orchid. BA with 0.5, 1.0, 1.5 and 2.0 mg/l and IBA with 0.5, 1.0, 1.5 and 2.0 mg/l were used in both varieties. Combined hormonal concentrations were also used. Shoot regeneration using nodal explants was found to be best in 2.0 mg/l BA supplementation on MS medium, which gives better responses than all other combinations of BA and BA+IBA concentrations under study in both D. bensoniae and D. aphyllum. In hormonal combination, highest number of shoots and leaves were found using 1.0 mg/l BA and 1.5 mg/l IBA along with MS medium in both varieties. The highest number and maximum length of roots were noticed using 1.5 mg/l IBA in D. bensoniae. Similarly, highest number and maximum length of roots were found in concentration of 2.0 mg/l in case of D. aphyllum. In hormonal combination, 0.5 mg/l BA and 1.0 mg/l IBA with MS medium found to be most effective for root induction in both varieties of D. bensoniae and D. aphyllum. The well-rooted plantlets were successfully acclimatized, planted in pots and transferred to the shade house for establishment. So MS medium supplemented with 2.0 mg/l BA may be used for rapid shoot induction, regeneration and multiplication and 0.5 BA+1.0 IBA (mg/l) supplemented with MS medium may be used for rapid induction of root in D. bensoniae and D. aphyllum. Sahida Siraj Riva M. Biotechnology 2017-02-27T10:43:42Z 2017-02-27T10:43:42Z 2016 2016-04 Thesis ID 13376002 http://hdl.handle.net/10361/7797 en BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 67 pages application/pdf BRAC University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Dendrobium bensoniae Dendrobium aphyllum |
| spellingShingle |
Dendrobium bensoniae Dendrobium aphyllum Riva, Sahida Siraj In Vitro regeneration and rapid multiplication of two orchid varieties of Dendrobium bensoniae and Dendrobium aphyllum |
| description |
A Dissertation Submitted in Partial Fulfillment of the Requirements’ for the Degree of Master of Science in Biotechnology 2016. |
| author2 |
Islam, Aparna |
| author_facet |
Islam, Aparna Riva, Sahida Siraj |
| format |
Thesis |
| author |
Riva, Sahida Siraj |
| author_sort |
Riva, Sahida Siraj |
| title |
In Vitro regeneration and rapid multiplication of two orchid varieties of Dendrobium bensoniae and Dendrobium aphyllum |
| title_short |
In Vitro regeneration and rapid multiplication of two orchid varieties of Dendrobium bensoniae and Dendrobium aphyllum |
| title_full |
In Vitro regeneration and rapid multiplication of two orchid varieties of Dendrobium bensoniae and Dendrobium aphyllum |
| title_fullStr |
In Vitro regeneration and rapid multiplication of two orchid varieties of Dendrobium bensoniae and Dendrobium aphyllum |
| title_full_unstemmed |
In Vitro regeneration and rapid multiplication of two orchid varieties of Dendrobium bensoniae and Dendrobium aphyllum |
| title_sort |
in vitro regeneration and rapid multiplication of two orchid varieties of dendrobium bensoniae and dendrobium aphyllum |
| publisher |
BRAC University |
| publishDate |
2017 |
| url |
http://hdl.handle.net/10361/7797 |
| work_keys_str_mv |
AT rivasahidasiraj invitroregenerationandrapidmultiplicationoftwoorchidvarietiesofdendrobiumbensoniaeanddendrobiumaphyllum |
| _version_ |
1814307768954257408 |