Multiplex PCR (polymerase chain reaction) assay for detection of E. coli O157:H7, Salmonella sp., Vibrio cholerae and Vibrio parahaemolyticus in spiked shrimps (Penaeus monodon)
This article was published in the Pakistan Journal of Biological Sciences [© 2013 Asian Network for Scientific Information] and the definite version is available at : http://dx.doi.org/10.3923/pjbs.2013.267.274. The Journal's website is at: http://scialert.net/abstract/index.php?doi=pjbs.2013.2...
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10361-71102016-12-04T09:45:45Z Multiplex PCR (polymerase chain reaction) assay for detection of E. coli O157:H7, Salmonella sp., Vibrio cholerae and Vibrio parahaemolyticus in spiked shrimps (Penaeus monodon) Fakruddin, M.D. Sultana, Mahmuda Ahmed, Monzur Morshed Chowdhury, Abhijit Choudhury, Naiyyum Department of Mathematics and Natural Sciences, BRAC University E. coli O157:H7 Multiplex PCR assay Salmonella spp Spiked shrimp Vibrio cholerae Vibrio parahaemolyticus This article was published in the Pakistan Journal of Biological Sciences [© 2013 Asian Network for Scientific Information] and the definite version is available at : http://dx.doi.org/10.3923/pjbs.2013.267.274. The Journal's website is at: http://scialert.net/abstract/index.php?doi=pjbs.2013.267.274 The coastal aquaculture mainly shrimps constitute major export sector in Bangladesh and is increasingly shaped by international trade conditions and by national responses to those stringent quality and safety standards. PCR based validated methods for detection of major bacterial pathogens in shrimp might be very useful tool for ensuring quality and safety standards of exportable shrimps. The objective of this study was to evaluate overall performance (sensitivity and specificity) of the multiplex PCR assay for detection of Vibrio cholerae, Vibrio parahaemolyticus, Salmonella sp. and Escherichia coli O157:H7 from spiked shrimp samples. The targeted genes were ompW for V. cholerae, tdh for V. parahaemolyticus, sefA for Salmonella spp. and hlyEHEC for E. coli O157:H7. The genomic DNA was extracted by using standard method and amplified accordingly. Sensitivity of the assay was tested by inoculating the shrimp homogenate with viable cells of laboratory references strains (target pathogens). The genes were amplified individually both from culture homogenate and spiked samples. Twenty different uniplex and multiplex PCR assay were performed; the results showed that the sensitivity and specificity of multiplex PCR are comparable to that of the results of uniplex PCR for the samples. DNA extracted from shrimp samples spiked with non-target pathogen (Bacillus cereus, Shigella flexneri and Staphylococcus aureus) yielded negative results. Published 2016-12-04T09:40:51Z 2016-12-04T09:40:51Z 2013 Article Fakruddin, M. D., Sultana, M., Ahmed, M. M., Chowdhury, A., & Choudhury, N. (2013). Multiplex PCR (polymerase chain reaction) assay for detection of E. coli O157:H7, salmonella sp., vibrio cholerae and vibrio parahaemolyticus in spiked shrimps (penaeus monodon). Pakistan Journal of Biological Sciences, 16(6), 267-274. doi:10.3923/pibs.2013.267.274 10288880 http://hdl.handle.net/10361/7110 http://dx.doi.org/10.3923/pjbs.2013.267.274 en http://scialert.net/abstract/index.php?doi=pjbs.2013.267.274 © 2013 Pakistan Journal of Biological Sciences |
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Brac University |
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Institutional Repository |
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English |
| topic |
E. coli O157:H7 Multiplex PCR assay Salmonella spp Spiked shrimp Vibrio cholerae Vibrio parahaemolyticus |
| spellingShingle |
E. coli O157:H7 Multiplex PCR assay Salmonella spp Spiked shrimp Vibrio cholerae Vibrio parahaemolyticus Fakruddin, M.D. Sultana, Mahmuda Ahmed, Monzur Morshed Chowdhury, Abhijit Choudhury, Naiyyum Multiplex PCR (polymerase chain reaction) assay for detection of E. coli O157:H7, Salmonella sp., Vibrio cholerae and Vibrio parahaemolyticus in spiked shrimps (Penaeus monodon) |
| description |
This article was published in the Pakistan Journal of Biological Sciences [© 2013 Asian Network for Scientific Information] and the definite version is available at : http://dx.doi.org/10.3923/pjbs.2013.267.274. The Journal's website is at: http://scialert.net/abstract/index.php?doi=pjbs.2013.267.274 |
| author2 |
Department of Mathematics and Natural Sciences, BRAC University |
| author_facet |
Department of Mathematics and Natural Sciences, BRAC University Fakruddin, M.D. Sultana, Mahmuda Ahmed, Monzur Morshed Chowdhury, Abhijit Choudhury, Naiyyum |
| format |
Article |
| author |
Fakruddin, M.D. Sultana, Mahmuda Ahmed, Monzur Morshed Chowdhury, Abhijit Choudhury, Naiyyum |
| author_sort |
Fakruddin, M.D. |
| title |
Multiplex PCR (polymerase chain reaction) assay for detection of E. coli O157:H7, Salmonella sp., Vibrio cholerae and Vibrio parahaemolyticus in spiked shrimps (Penaeus monodon) |
| title_short |
Multiplex PCR (polymerase chain reaction) assay for detection of E. coli O157:H7, Salmonella sp., Vibrio cholerae and Vibrio parahaemolyticus in spiked shrimps (Penaeus monodon) |
| title_full |
Multiplex PCR (polymerase chain reaction) assay for detection of E. coli O157:H7, Salmonella sp., Vibrio cholerae and Vibrio parahaemolyticus in spiked shrimps (Penaeus monodon) |
| title_fullStr |
Multiplex PCR (polymerase chain reaction) assay for detection of E. coli O157:H7, Salmonella sp., Vibrio cholerae and Vibrio parahaemolyticus in spiked shrimps (Penaeus monodon) |
| title_full_unstemmed |
Multiplex PCR (polymerase chain reaction) assay for detection of E. coli O157:H7, Salmonella sp., Vibrio cholerae and Vibrio parahaemolyticus in spiked shrimps (Penaeus monodon) |
| title_sort |
multiplex pcr (polymerase chain reaction) assay for detection of e. coli o157:h7, salmonella sp., vibrio cholerae and vibrio parahaemolyticus in spiked shrimps (penaeus monodon) |
| publisher |
© 2013 Pakistan Journal of Biological Sciences |
| publishDate |
2016 |
| url |
http://hdl.handle.net/10361/7110 http://dx.doi.org/10.3923/pjbs.2013.267.274 |
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