Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (Psidium cattleianum) leaves on H2O2-treated HepG2 cell
This article was published in Journal of the Korean Society for Applied Biological Chemistry [© 2013 Springer Link] and the definite version is available at: http://link.springer.com/article/10.1007/s13765-013-3156-z The article website is at: http://link.springer.com/journal/13765
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© 2013 Springer Link
2016
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| Acceso en línea: | http://hdl.handle.net/10361/6939 |
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10361-69392016-11-23T09:28:48Z Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (Psidium cattleianum) leaves on H2O2-treated HepG2 cell Mosaddik, Ashik Moon, Jeong Yong Lee, Sarah Jeong, Seongweon Kim, Jong-Chan Ahn, Kwang Seok Cho, Somi Kim Department of Pharmacy, BRAC University Antioxidants Free radical HepG2 Guava leaf Polyphenol This article was published in Journal of the Korean Society for Applied Biological Chemistry [© 2013 Springer Link] and the definite version is available at: http://link.springer.com/article/10.1007/s13765-013-3156-z The article website is at: http://link.springer.com/journal/13765 Total phenolic contents of different fractions of the Psidium cattleianum leaf extract and their antioxidant capacity against several free radicals were examined. Protective effect of the ethyl acetate fraction (EAF) on H2O2-induced DNA damage in HepG2 cells were also evaluated, and the phytochemical profile of EAF was analyzed using tandem mass spectrometry. EAF derived from the 80% methanol extract of the leaf contained a remarkable amount of polyphenol and showed high levels of DPPH and alkyl radical scavenging activity, promoted cell viability, and protected against H2O2-induced DNA damage in HepG2 cells. Phytochemical analysis revealed that the major components in the EAF included quercetin monoglycoside, phloridizin, quercetin 3-diglycoside, quercetin-3-glucuronide, 2,6-dihydroxy-3,5-dimethyl-4-glucopyranosylbenzophenone, phenolic acid, guaijaverin, and naringin. The present study suggests possible synergistic or competitive antioxidant action of the major compounds of cattley guava leaf on H2O2-induced DNA damage in HepG2 cells. These results indicate that the ethyl acetate fraction of the guava leaf could be used as a potential source of natural antioxidants, and these findings will facilitate the utilization of guava leaf as a source of functional food. Published 2016-11-23T09:21:27Z 2016-11-23T09:21:27Z 2013-12 Article Moon, J. Y., Lee, S., Jeong, S., Kim, J. -., Ahn, K. S., Mosaddik, A., & Cho, S. K. (2013). Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (psidium cattleianum) leaves on H2O2-treated HepG2 cell. Journal of the Korean Society for Applied Biological Chemistry, 56(6), 687-694. doi:10.1007/s13765-013-3156-z 17382203 http://hdl.handle.net/10361/6939 en http://link.springer.com/article/10.1007/s13765-013-3156-z#article-dates-history © 2013 Springer Link |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Antioxidants Free radical HepG2 Guava leaf Polyphenol |
| spellingShingle |
Antioxidants Free radical HepG2 Guava leaf Polyphenol Mosaddik, Ashik Moon, Jeong Yong Lee, Sarah Jeong, Seongweon Kim, Jong-Chan Ahn, Kwang Seok Cho, Somi Kim Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (Psidium cattleianum) leaves on H2O2-treated HepG2 cell |
| description |
This article was published in Journal of the Korean Society for Applied Biological Chemistry [© 2013 Springer Link] and the definite version is available at: http://link.springer.com/article/10.1007/s13765-013-3156-z The article website is at: http://link.springer.com/journal/13765 |
| author2 |
Department of Pharmacy, BRAC University |
| author_facet |
Department of Pharmacy, BRAC University Mosaddik, Ashik Moon, Jeong Yong Lee, Sarah Jeong, Seongweon Kim, Jong-Chan Ahn, Kwang Seok Cho, Somi Kim |
| format |
Article |
| author |
Mosaddik, Ashik Moon, Jeong Yong Lee, Sarah Jeong, Seongweon Kim, Jong-Chan Ahn, Kwang Seok Cho, Somi Kim |
| author_sort |
Mosaddik, Ashik |
| title |
Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (Psidium cattleianum) leaves on H2O2-treated HepG2 cell |
| title_short |
Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (Psidium cattleianum) leaves on H2O2-treated HepG2 cell |
| title_full |
Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (Psidium cattleianum) leaves on H2O2-treated HepG2 cell |
| title_fullStr |
Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (Psidium cattleianum) leaves on H2O2-treated HepG2 cell |
| title_full_unstemmed |
Free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (Psidium cattleianum) leaves on H2O2-treated HepG2 cell |
| title_sort |
free radical-scavenging activities and cytoprotective effect of polyphenol-rich ethyl acetate fraction of guava (psidium cattleianum) leaves on h2o2-treated hepg2 cell |
| publisher |
© 2013 Springer Link |
| publishDate |
2016 |
| url |
http://hdl.handle.net/10361/6939 |
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