To control site-specific skin gene expression, autocrine mimics paracrine canonical Wnt signaling and Is activated ectopically in skin disease
This article was published in American Journal of Pathology, [© 2016 American Society for Investigative Pathology] and the definite version is available at https://jhu.pure.elsevier.com/en/publications/to-control-site-specific-skin-gene-expression-autocrine-mimics-pa The article website is at: http...
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© 2016 American Society for Investigative Pathology
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| Առցանց հասանելիություն: | http://hdl.handle.net/10361/6795 |
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10361-67952016-11-29T09:29:27Z To control site-specific skin gene expression, autocrine mimics paracrine canonical Wnt signaling and Is activated ectopically in skin disease Kim, Dongwon M. Zulfiquer Hossain Nieves, Ashley Gu, Lihong Ratliff, Tabetha S. Oh, Seung Mi Park, Angela Han, Seunghyun Yang, Nicole B. Qi, Ji Taube, Janis M. Kang, Sewon Garza, Luis A. Department of Pharmacy, BRAC University Skin gene expression Skin disease This article was published in American Journal of Pathology, [© 2016 American Society for Investigative Pathology] and the definite version is available at https://jhu.pure.elsevier.com/en/publications/to-control-site-specific-skin-gene-expression-autocrine-mimics-pa The article website is at: http://ajp.amjpathol.org/article/S0002-9440(16)00136-X/fulltext Despite similar components, the heterogeneity of skin characteristics across the human body is enormous. It is classically believed that site-specific fibroblasts in the dermis control postnatal skin identity by modulating the behavior of the surface-overlying keratinocytes in the epidermis. To begin testing this hypothesis, we characterized the gene expression differences between volar (ventral; palmoplantar) and nonvolar (dorsal) human skin. We show that KERATIN 9 (KRT9) is the most uniquely enriched transcript in volar skin, consistent with its etiology in genetic diseases of the palms and soles. In addition, ectopic KRT9 expression is selectively activated by volar fibroblasts. However, KRT9 expression occurs in the absence of all fibroblasts, although not to the maximal levels induced by fibroblasts. Through gain-of-function and loss-of-function experiments, we demonstrate that the mechanism is through overlapping paracrine or autocrine canonical WNT-β-catenin signaling in each respective context. Finally, as an in vivo example of ectopic expression of KRT9 independent of volar fibroblasts, we demonstrate that in the human skin disease lichen simplex chronicus, WNT5a and KRT9 are robustly activated outside of volar sites. These results highlight the complexities of site-specific gene expression and its disruption in skin disease. © 2016 American Society for Investigative Pathology. Published 2016-11-09T07:06:49Z 2016-11-09T07:06:49Z 2016 Article Kim, D., Hossain, M. Z., Nieves, A., Gu, L., Ratliff, T. S., Mi Oh, S., . . . Garza, L. A. (2016). To control site-specific skin gene expression, autocrine mimics paracrine canonical wnt signaling and is activated ectopically in skin disease. American Journal of Pathology, 186(5), 1140-1150. doi:10.1016/j.ajpath.2015.12.030 00029440 http://hdl.handle.net/10361/6795 10.1016/j.ajpath.2015.12.030 https://jhu.pure.elsevier.com/en/publications/to-control-site-specific-skin-gene-expression-autocrine-mimics-pa © 2016 American Society for Investigative Pathology |
| institution |
Brac University |
| collection |
Institutional Repository |
| topic |
Skin gene expression Skin disease |
| spellingShingle |
Skin gene expression Skin disease Kim, Dongwon M. Zulfiquer Hossain Nieves, Ashley Gu, Lihong Ratliff, Tabetha S. Oh, Seung Mi Park, Angela Han, Seunghyun Yang, Nicole B. Qi, Ji Taube, Janis M. Kang, Sewon Garza, Luis A. To control site-specific skin gene expression, autocrine mimics paracrine canonical Wnt signaling and Is activated ectopically in skin disease |
| description |
This article was published in American Journal of Pathology, [© 2016 American Society for Investigative Pathology] and the definite version is available at https://jhu.pure.elsevier.com/en/publications/to-control-site-specific-skin-gene-expression-autocrine-mimics-pa The article website is at: http://ajp.amjpathol.org/article/S0002-9440(16)00136-X/fulltext |
| author2 |
Department of Pharmacy, BRAC University |
| author_facet |
Department of Pharmacy, BRAC University Kim, Dongwon M. Zulfiquer Hossain Nieves, Ashley Gu, Lihong Ratliff, Tabetha S. Oh, Seung Mi Park, Angela Han, Seunghyun Yang, Nicole B. Qi, Ji Taube, Janis M. Kang, Sewon Garza, Luis A. |
| format |
Article |
| author |
Kim, Dongwon M. Zulfiquer Hossain Nieves, Ashley Gu, Lihong Ratliff, Tabetha S. Oh, Seung Mi Park, Angela Han, Seunghyun Yang, Nicole B. Qi, Ji Taube, Janis M. Kang, Sewon Garza, Luis A. |
| author_sort |
Kim, Dongwon |
| title |
To control site-specific skin gene expression, autocrine mimics paracrine canonical Wnt signaling and Is activated ectopically in skin disease |
| title_short |
To control site-specific skin gene expression, autocrine mimics paracrine canonical Wnt signaling and Is activated ectopically in skin disease |
| title_full |
To control site-specific skin gene expression, autocrine mimics paracrine canonical Wnt signaling and Is activated ectopically in skin disease |
| title_fullStr |
To control site-specific skin gene expression, autocrine mimics paracrine canonical Wnt signaling and Is activated ectopically in skin disease |
| title_full_unstemmed |
To control site-specific skin gene expression, autocrine mimics paracrine canonical Wnt signaling and Is activated ectopically in skin disease |
| title_sort |
to control site-specific skin gene expression, autocrine mimics paracrine canonical wnt signaling and is activated ectopically in skin disease |
| publisher |
© 2016 American Society for Investigative Pathology |
| publishDate |
2016 |
| url |
http://hdl.handle.net/10361/6795 |
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