Comparison of Lipopolysaccharide (LPS) specific IgA and IgG avidity maturation between Vivotif Vaccinees and naturally infected enteric patients in Bangladesh

A Dissertation Submitted in Partial Fulfillment of the Requirements’ for the Degree of Master of Science in Biotechnology

Detalhes bibliográficos
Autor principal:	Rashed, Faisal Bin
Outros Autores:	Choudhury, Naiyyum
Formato:	Tese
Idioma:	English
Publicado em:	BRAC University 2016
Assuntos:	Biotechnology Vivotif vaccinees
Acesso em linha:	http://hdl.handle.net/10361/4822

id	10361-4822
record_format	dspace
spelling	10361-48222019-09-30T04:23:48Z Comparison of Lipopolysaccharide (LPS) specific IgA and IgG avidity maturation between Vivotif Vaccinees and naturally infected enteric patients in Bangladesh Rashed, Faisal Bin Choudhury, Naiyyum Qadri, Firdausi Department of Mathematics and Natural Sciences, BRAC University Biotechnology Vivotif vaccinees A Dissertation Submitted in Partial Fulfillment of the Requirements’ for the Degree of Master of Science in Biotechnology Cataloged from PDF version of thesis report. Includes bibliographical references (page 75-87). Enteric fever, primarily caused by Salmonella enterica serovar Typhi and Paratyphi, is a potentially life-threatening systemic disease. Characterized by fever, malaise and diffused abdominal pain, it can lead to delirium, intestinal hemorrhage, bowel perforation, and even death if left untreated. High prevalence rates of enteric fever have been reported in the resource limited regions of the world, with children under 5 years of age being the largest target. Poor sensitivity and specificity of conventional diagnostic approaches result in lower or late detection of the infection. The TPTest, a novel diagnostic technique, has proved to be highly sensitive and specific for detection of the patients with enteric fever. Vaccination also is potentially important to reduce the incidence of enteric fever among young children. Vivotif, a live oral attenuated typhoid vaccine, has undergone clinical trials in different countries, including Bangladesh. However, no data on the analysis of the immune response or longevity has been carried out. The antibody avidity maturation in typhoid vaccinees or patients has also not been reported till date. Under these circumstances, this study demonstrates the lipopolysaccharide (LPS) specific immunoglobulin A (IgA) and immunoglobulin G (IgG) avidity maturation in Vivotif vaccinees and naturally infected typhoid patients aged below 5 years. Also, the age dependent variation in avidity maturation was analyzed, both in S. Typhi bacteremic and only TPTest positive patients. Vaccination with Vivotif generated IgA and IgG antibodies with significantly higher avidity that lasted throughout the study period of 28 days. Patients with S. Typhi bacteremia with an age below 5 years showed a higher IgA avidity index in the first two study day points, followed by a decrease on the second follow-up. Overall, culture positive patients had a higher IgA avidity index compared to the only TPTest positive patients, regardless of age. A similar response for IgG avidity maturation was seen among patients. Lack of significant differences in IgG avidity between different day points within a group could be attributed to the longer half-life of IgG. In short, S. Typhi bacteremic patients produced more highly avid IgA and IgG antibodies against LPS compared to only TPTest positive patients, indicating that higher bacterial load in the system may aid in generation of higher avidity antibodies. Faisal Bin Rashed M. Biotechnology 2016-01-14T15:00:01Z 2016-01-14T15:00:01Z 2014 2014-08 Thesis ID 13376003 http://hdl.handle.net/10361/4822 en BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 87 pages application/pdf BRAC University
institution	Brac University
collection	Institutional Repository
language	English
topic	Biotechnology Vivotif vaccinees
spellingShingle	Biotechnology Vivotif vaccinees Rashed, Faisal Bin Comparison of Lipopolysaccharide (LPS) specific IgA and IgG avidity maturation between Vivotif Vaccinees and naturally infected enteric patients in Bangladesh
description	A Dissertation Submitted in Partial Fulfillment of the Requirements’ for the Degree of Master of Science in Biotechnology
author2	Choudhury, Naiyyum
author_facet	Choudhury, Naiyyum Rashed, Faisal Bin
format	Thesis
author	Rashed, Faisal Bin
author_sort	Rashed, Faisal Bin
title	Comparison of Lipopolysaccharide (LPS) specific IgA and IgG avidity maturation between Vivotif Vaccinees and naturally infected enteric patients in Bangladesh
title_short	Comparison of Lipopolysaccharide (LPS) specific IgA and IgG avidity maturation between Vivotif Vaccinees and naturally infected enteric patients in Bangladesh
title_full	Comparison of Lipopolysaccharide (LPS) specific IgA and IgG avidity maturation between Vivotif Vaccinees and naturally infected enteric patients in Bangladesh
title_fullStr	Comparison of Lipopolysaccharide (LPS) specific IgA and IgG avidity maturation between Vivotif Vaccinees and naturally infected enteric patients in Bangladesh
title_full_unstemmed	Comparison of Lipopolysaccharide (LPS) specific IgA and IgG avidity maturation between Vivotif Vaccinees and naturally infected enteric patients in Bangladesh
title_sort	comparison of lipopolysaccharide (lps) specific iga and igg avidity maturation between vivotif vaccinees and naturally infected enteric patients in bangladesh
publisher	BRAC University
publishDate	2016
url	http://hdl.handle.net/10361/4822
work_keys_str_mv	AT rashedfaisalbin comparisonoflipopolysaccharidelpsspecificigaandiggaviditymaturationbetweenvivotifvaccineesandnaturallyinfectedentericpatientsinbangladesh
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Comparison of Lipopolysaccharide (LPS) specific IgA and IgG avidity maturation between Vivotif Vaccinees and naturally infected enteric patients in Bangladesh

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