Extraction of secondary metabolites, phytochemical screening and the analysis of antibacterial activity in stevia rebaudiana
This thesis report is submitted in partial fulfillment of the requirement for the degree of Bachelor of Science in Biotechnology, 2015.
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BRAC University
2015
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10361-45492019-09-30T04:12:22Z Extraction of secondary metabolites, phytochemical screening and the analysis of antibacterial activity in stevia rebaudiana Zohra, Fatima Tuz Chowdhury, Ms. Jebunnesa Department of Mathematical and Natural Science, BRAC University Mathematics and Natural Sciences Biotechnology Stevia rebaudiana This thesis report is submitted in partial fulfillment of the requirement for the degree of Bachelor of Science in Biotechnology, 2015. Cataloged from PDF version of thesis. Includes bibliographical references (page 52-53). Stevia rebaudiana is a plant of the Asteraceae family and native to Paraguay. Its sweetness and calorie free property has increased its demand commercially all over the world as a sugar substitute in food beverages and medicines. Stevia has many pharmacological and therapeutic properties including antimicrobial, antifungal, antioxidant and anticarcinogenic activity. Due to this importance the leaf was analyzed for preliminary phytochemical studies to find the presence of phytoconstituents such as alkaloids, flavonoids, steroidal compounds, sponins, tannins, phenols, and cardiac glycosides. In this study, the crude plant extract from three different extractions (ethanol, methanol, and water) were collected and tested for the presence of phytochemicals. Comparative analysis of the antimicrobial activity of the extracts was investigated against four bacterial strains (Bacillus subtilis, Staphylococcus aureus, Streptococus peneumoniae and Shigella flexnari). This was done using agar diffusion method and as controls kanamycin, tetracycline and chloramphenicol were used. The diameter of the clear zone of inhibition surrounding the disc was measured in millimeters. The results showed that methanolic and ethanolic extracts of Stevia rebaudiana contained the highest amounts of phytochemicals. Antibacterial activity results showed there is no inhibitory effect of stevia against S.aureus and S.flexnari. The highest amount inhibition was formed against B.subtilis and S.pneumoniae. It can be concluded from the results that methanolic, ethanolic and aqueous extracts of Stevia rebaudiana may be considered as an antibacterial agent against S.pneumoniae and B.subtilis and be used to source antibiotic substances. Fatima Tuz Zohra B. Biotechnology 2015-10-31T06:59:56Z 2015-10-31T06:59:56Z 2015 2015-09 Thesis ID 11136012 http://hdl.handle.net/10361/4549 en BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 55 pages application/pdf BRAC University |
| institution |
Brac University |
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Institutional Repository |
| language |
English |
| topic |
Mathematics and Natural Sciences Biotechnology Stevia rebaudiana |
| spellingShingle |
Mathematics and Natural Sciences Biotechnology Stevia rebaudiana Zohra, Fatima Tuz Extraction of secondary metabolites, phytochemical screening and the analysis of antibacterial activity in stevia rebaudiana |
| description |
This thesis report is submitted in partial fulfillment of the requirement for the degree of Bachelor of Science in Biotechnology, 2015. |
| author2 |
Chowdhury, Ms. Jebunnesa |
| author_facet |
Chowdhury, Ms. Jebunnesa Zohra, Fatima Tuz |
| format |
Thesis |
| author |
Zohra, Fatima Tuz |
| author_sort |
Zohra, Fatima Tuz |
| title |
Extraction of secondary metabolites, phytochemical screening and the analysis of antibacterial activity in stevia rebaudiana |
| title_short |
Extraction of secondary metabolites, phytochemical screening and the analysis of antibacterial activity in stevia rebaudiana |
| title_full |
Extraction of secondary metabolites, phytochemical screening and the analysis of antibacterial activity in stevia rebaudiana |
| title_fullStr |
Extraction of secondary metabolites, phytochemical screening and the analysis of antibacterial activity in stevia rebaudiana |
| title_full_unstemmed |
Extraction of secondary metabolites, phytochemical screening and the analysis of antibacterial activity in stevia rebaudiana |
| title_sort |
extraction of secondary metabolites, phytochemical screening and the analysis of antibacterial activity in stevia rebaudiana |
| publisher |
BRAC University |
| publishDate |
2015 |
| url |
http://hdl.handle.net/10361/4549 |
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AT zohrafatimatuz extractionofsecondarymetabolitesphytochemicalscreeningandtheanalysisofantibacterialactivityinsteviarebaudiana |
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