Establishment of transformation protocol and regeneration potential comparison following introduction of the antiporter gene in peanut (Arachis hypogaea L.)

A Dissertation Submitted in Partial Fulfillment of the Requirements’ for the Degree of Master of Science in Biotechnology

Détails bibliographiques
Auteur principal:	Khan, Kashmery
Autres auteurs:	Islam, Dr. Aparna
Format:	Thèse
Langue:	English
Publié:	BRAC University 2015
Sujets:	Mathematics and Natural Sciences Biotechnology
Accès en ligne:	http://hdl.handle.net/10361/4528

id	10361-4528
record_format	dspace
spelling	10361-45282019-09-30T04:22:15Z Establishment of transformation protocol and regeneration potential comparison following introduction of the antiporter gene in peanut (Arachis hypogaea L.) Khan, Kashmery Islam, Dr. Aparna Department of Mathematical and Natural Science, BRAC University Mathematics and Natural Sciences Biotechnology A Dissertation Submitted in Partial Fulfillment of the Requirements’ for the Degree of Master of Science in Biotechnology Cataloged from PDF version of thesis report. Includes bibliographical references (page 77-87). Peanut is a cash crop which is cultivated in 24 million ha all over the world for extracting edible oil. It is a great food having outstanding food values, specially for hunger and malnutrition affecting Asian countries like Bangladesh. But peanut yield is substantially reduced because of abiotic stresses like salinity. As a result, salt tolerance in peanut has to be improved to ensure better production where the success of conventional breeding severely hampered due to its cleistogamous nature of pollination. Therefore, Agrobacterium- mediated genetic transformation may lead the solution. On that context, present investigation was carried out with three Bangladeshi peanut varieties, such as, BINA Chinabadam 2, BINA Chinabadam 4 and BINA Chinabadam 6 using two explants, named, decapitated half embryo and embryonic leaflet. Factors influencing transformation and comparing the regeneration potential of explants on selection media `was done using three different vectors, named, pBI121, pK7WG2_OsNHX1_1.6 & pK7WG2_AtNHX1_1.6. Decapitated half embryo showed better in vitro regeneration potential than leaflet explant. Hence, transformation study was conducted with 1 day old decapitated half embryo where highest percentage of GUS positive explants were found when OD600 was more than 1 at 60 minutes of infection and 3 days of co-cultivation duration in transient assay. Regeneration efficiency on selection media containing 50mg/l kanamycin was higher in almost all three varieties. In contrast to this, untransformed control explants were able to resist upto 20mg/l of kanamycin. Moreover, average number of putative transformed shoots was gradually dropped with the increased concentration of kanamycin. Shoots which survived upto 150mg/l kanamycin were transferred into rooting media for root induction. Finally transformation efficiency was evaluated by calculating the percentage of GUS positive plant parts, such as, leaf, stem and roots among the putatively transformed plantlets. Maximum transformation frequency (14.28%) was observed in BINA Chinabadam 4. In the current experiment, effect of salinity on peanut seed germination was also observed. The germination rate steadily decreased with the increase of NaCl in the media and finally no germination was observed in MS media supplemented with 150mM of NaCl and above. M. Biotechnology 2015-10-29T08:25:39Z 2015-10-29T08:25:39Z 2015 2015-09 Thesis ID 12176002 http://hdl.handle.net/10361/4528 en BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 87 pages application/pdf BRAC University
institution	Brac University
collection	Institutional Repository
language	English
topic	Mathematics and Natural Sciences Biotechnology
spellingShingle	Mathematics and Natural Sciences Biotechnology Khan, Kashmery Establishment of transformation protocol and regeneration potential comparison following introduction of the antiporter gene in peanut (Arachis hypogaea L.)
description	A Dissertation Submitted in Partial Fulfillment of the Requirements’ for the Degree of Master of Science in Biotechnology
author2	Islam, Dr. Aparna
author_facet	Islam, Dr. Aparna Khan, Kashmery
format	Thesis
author	Khan, Kashmery
author_sort	Khan, Kashmery
title	Establishment of transformation protocol and regeneration potential comparison following introduction of the antiporter gene in peanut (Arachis hypogaea L.)
title_short	Establishment of transformation protocol and regeneration potential comparison following introduction of the antiporter gene in peanut (Arachis hypogaea L.)
title_full	Establishment of transformation protocol and regeneration potential comparison following introduction of the antiporter gene in peanut (Arachis hypogaea L.)
title_fullStr	Establishment of transformation protocol and regeneration potential comparison following introduction of the antiporter gene in peanut (Arachis hypogaea L.)
title_full_unstemmed	Establishment of transformation protocol and regeneration potential comparison following introduction of the antiporter gene in peanut (Arachis hypogaea L.)
title_sort	establishment of transformation protocol and regeneration potential comparison following introduction of the antiporter gene in peanut (arachis hypogaea l.)
publisher	BRAC University
publishDate	2015
url	http://hdl.handle.net/10361/4528
work_keys_str_mv	AT khankashmery establishmentoftransformationprotocolandregenerationpotentialcomparisonfollowingintroductionoftheantiportergeneinpeanutarachishypogaeal
_version_	1814308929136492544

Establishment of transformation protocol and regeneration potential comparison following introduction of the antiporter gene in peanut (Arachis hypogaea L.)

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