A comparative analysis on antibiotic resistance pattern in environmental and clinical bacterial isolates based on whole cell protein profiling by SDS-PAGE

This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2015

Chi tiết về thư mục
Tác giả chính:	Fatema, Aneeka Nawar
Định dạng:	Luận văn
Ngôn ngữ:	English
Được phát hành:	BRAC University 2015
Những chủ đề:	Microbiology Antibiotic resistance pattern SDS-PAGE Cell Protein Profiling
Truy cập trực tuyến:	http://hdl.handle.net/10361/4476

id	10361-4476
record_format	dspace
spelling	10361-44762019-09-30T04:13:25Z A comparative analysis on antibiotic resistance pattern in environmental and clinical bacterial isolates based on whole cell protein profiling by SDS-PAGE Fatema, Aneeka Nawar Microbiology Antibiotic resistance pattern SDS-PAGE Cell Protein Profiling This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2015 This comparative study was carried out to investigate the presence of antibiotic sensitive and resistant bacteria in different environmental samples of Bangladesh with specific clinical strain obtained from ICDDR, B. The environmental samples were isolated from different locations of Dhaka city to perceive the propensity of the environmental isolates to develop antibiotic resistance. The bacterial isolates were identified on the basis of standard cultural, morphological and biochemical characteristics. Antibiogram was done to identify the antibiotic susceptibility of the isolates according to Clinical and Laboratory Standards Institute (CLSI) guideline. The result of this study indicated all the environmental isolates have become resistant to the tested antibiotics, and some of them have become multi-resistant to these therapeutic agents whereas all the clinical isolates showed the opposite result. The study helps to predict the future emergence and guide the development of strategies to counteract this resistance. Therefore comprehensive survey of antibiotic resistance in the environmental bacteria is required. This comparative study was carried out to investigate the difference in protein band of resistant strain of E.coli and Staphylococcus aureus isolated from different environmental samples of Bangladesh along with specific clinical strain obtained from ICDDR, B of E.coli and Staphylococcus aureus. The antibiotic resistance of the identified organisms was carried out by disc diffusion method with commercially available disc of Sulfamethoxazole / Trimethoprim (SXT 25), Cefoxitin (FOX 30), Pefloxacin (PEF 5), Ciprofloxacin (CIP 5), Erythromycin (E 15), Gentamicin (CN 10), Kanamycin (K 30), Streptomycin (S 10), Cefuroxime Sodium (CXM 30), Nalidixic Acid (NA 30), Oxacillin (OX 1), Chloramphenicol (C 30), Nitrofurantoin (F 300). Antibiotics have different mode of action such as cell wall synthesis inhibitors, membrane permeability alternatives, protein synthesis inhibitors and DNA synthesis inhibitors. It was further studied by using crude extraction of whole cell proteins followed by separation of proteins by means of SDS-PAGE. The environmental samples were isolated from different locations of Dhaka city to perceive the propensity of the environmental isolates to develop antibiotic resistance at different time periods of a year. The bacterial isolates were identified on the basis of standard cultural, morphological and biochemical characteristics. Antibiogram was done to identify the antibiotic susceptibility of the isolates according to CLSI guideline. The result of this study indicated that there is a specific protein band difference of the environmental isolates and the clinical isolate. The study helps to predict the future emergence and guide the development of strategies to counteract this resistance. Therefore periodic and comprehensive survey of antibiotic resistance in the environmental bacteria is required. 2015-10-07T04:59:11Z 2015-10-07T04:59:11Z 2015-08 Thesis ID 11126003 http://hdl.handle.net/10361/4476 en application/pdf BRAC University
institution	Brac University
collection	Institutional Repository
language	English
topic	Microbiology Antibiotic resistance pattern SDS-PAGE Cell Protein Profiling
spellingShingle	Microbiology Antibiotic resistance pattern SDS-PAGE Cell Protein Profiling Fatema, Aneeka Nawar A comparative analysis on antibiotic resistance pattern in environmental and clinical bacterial isolates based on whole cell protein profiling by SDS-PAGE
description	This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2015
format	Thesis
author	Fatema, Aneeka Nawar
author_facet	Fatema, Aneeka Nawar
author_sort	Fatema, Aneeka Nawar
title	A comparative analysis on antibiotic resistance pattern in environmental and clinical bacterial isolates based on whole cell protein profiling by SDS-PAGE
title_short	A comparative analysis on antibiotic resistance pattern in environmental and clinical bacterial isolates based on whole cell protein profiling by SDS-PAGE
title_full	A comparative analysis on antibiotic resistance pattern in environmental and clinical bacterial isolates based on whole cell protein profiling by SDS-PAGE
title_fullStr	A comparative analysis on antibiotic resistance pattern in environmental and clinical bacterial isolates based on whole cell protein profiling by SDS-PAGE
title_full_unstemmed	A comparative analysis on antibiotic resistance pattern in environmental and clinical bacterial isolates based on whole cell protein profiling by SDS-PAGE
title_sort	comparative analysis on antibiotic resistance pattern in environmental and clinical bacterial isolates based on whole cell protein profiling by sds-page
publisher	BRAC University
publishDate	2015
url	http://hdl.handle.net/10361/4476
work_keys_str_mv	AT fatemaaneekanawar acomparativeanalysisonantibioticresistancepatterninenvironmentalandclinicalbacterialisolatesbasedonwholecellproteinprofilingbysdspage AT fatemaaneekanawar comparativeanalysisonantibioticresistancepatterninenvironmentalandclinicalbacterialisolatesbasedonwholecellproteinprofilingbysdspage
_version_	1814308185219006464

A comparative analysis on antibiotic resistance pattern in environmental and clinical bacterial isolates based on whole cell protein profiling by SDS-PAGE

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