Study on SOD1 GENE +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin

This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2014.

Détails bibliographiques
Auteur principal:	Shahriar, S.M.
Langue:	English
Publié:	BRAC University 2015
Sujets:	Biotechnology
Accès en ligne:	http://hdl.handle.net/10361/4151

id	10361-4151
record_format	dspace
spelling	10361-41512019-09-30T04:22:20Z Study on SOD1 GENE +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin Shahriar, S.M. Biotechnology This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2014. Superoxide dismutase 1 (SOD1), located at the cytosol has been postulated to represent between 50%-80% of the total SOD activity. The SOD gene is located at 21q22.1. Polymorphisms in the genes encoding these enzymes found to be significantly affecting its antioxidant role. As oxidative stress is a common pathogenic factor for the dysfunction of beta and endothelial cells, polymorphism of SOD genes and oxidative stress has become a subject of intense scrutiny for their association with different disease conditions including T2DM. The study was aimed to assess the possible association of SOD1 gene +35A/C polymorphism in the pathogenesis of IGR subjects of Bangladeshi origin. A total number of 54 middle aged (age range: 30-65year) subjects with Impaired Glucose Regulation (IGR) were consecutively recruited. Total number of healthy control was 55. IGR was diagnosed following WHO guideline. Blood glucose was measured by glucose-oxidase and, triglyceride, total cholesterol and HDL-cholesterol by enzymatic colorimetric method. Insulin was estimated by enzyme linked immunosorbent assay (ELISA). DNA was extracted using QIAGEN Blood DNA Kit which utilizes silica gel DNA separation. SOD1 gene +35A/C variant was determined by PCR-RFLP using restriction endonuclease HhaI. Data were managed using Statistical Program for Social Science (SPSS). Unpaired Student's -`t' test and Chi-squared tests, as appropriate, were performed. The number of study subjects of wild AA genotype in control and IGR group was 52 and 54 respectively whereas in case of heterozygous (Ht) variant AC genotype the number was 0 and 2 respectively. SOD1 +35A>C genotype frequency analysis has not shown statistical significant association (χ2 = 2.075 and p= 0.150). For control group, the genotype frequencies of AA and AC were 1.0 and 0 respectively. For IGR group, the frequencies were 0.963 and 0.037 respectively. The data showed that SOD1 gene +35A>C variant is substantially different from other population and the polymorphic allele is not associated with insulin secretory defect of IGR subjects of Bangladeshi origin. 2015-05-26T05:09:32Z 2015-05-26T05:09:32Z 2014-07 ID 12176007 http://hdl.handle.net/10361/4151 en application/pdf BRAC University
institution	Brac University
collection	Institutional Repository
language	English
topic	Biotechnology
spellingShingle	Biotechnology Shahriar, S.M. Study on SOD1 GENE +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin
description	This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2014.
author	Shahriar, S.M.
author_facet	Shahriar, S.M.
author_sort	Shahriar, S.M.
title	Study on SOD1 GENE +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin
title_short	Study on SOD1 GENE +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin
title_full	Study on SOD1 GENE +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin
title_fullStr	Study on SOD1 GENE +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin
title_full_unstemmed	Study on SOD1 GENE +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin
title_sort	study on sod1 gene +35a>c polymorphism in impaired glucose regulation patients of bangladeshi origin
publisher	BRAC University
publishDate	2015
url	http://hdl.handle.net/10361/4151
work_keys_str_mv	AT shahriarsm studyonsod1gene35acpolymorphisminimpairedglucoseregulationpatientsofbangladeshiorigin
_version_	1814309676067586048

Study on SOD1 GENE +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin

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