In vitro culture establishment from Cotyledon and Embryonic Axis Explants of two Bangladeshi Sunflower (Helianthus annus L.) varieties

In vitro culture establishment from Cotyledon and Embryonic Axis Explants of two Bangladeshi Sunflower (Helianthus annus L.) varieties

This thesis report is submitted in partial fulfillment of the requirement for the degree of Bachelor of Science in Biotechnology, 2015.

Dades bibliogràfiques
Autor principal: Chowdhury, Maisha Mosharrat
Altres autors: Islam, Aparna
Format: Thesis
Idioma:English
Publicat: BRAC University 2015
Matèries:
Biotechnology
Accés en línia:http://hdl.handle.net/10361/4129
id 10361-4129
record_format dspace
spelling 10361-41292019-09-30T04:12:17Z In vitro culture establishment from Cotyledon and Embryonic Axis Explants of two Bangladeshi Sunflower (Helianthus annus L.) varieties Chowdhury, Maisha Mosharrat Islam, Aparna Department of Mathematical and Natural Science, BRAC University Biotechnology This thesis report is submitted in partial fulfillment of the requirement for the degree of Bachelor of Science in Biotechnology, 2015. Cataloged from PDF version of thesis. Includes bibliographical references (page 48 - 51). Sunflower is considered as source of cholesterol free edible oil. Because of cross pollinating nature of this plant, it is difficult to identify true breeding homozygous line, thus, breeding becomes intricate. Besides, due to biotic and abiotic stresses, development of this crop is urgent. This study was aimed to develop a potential alternative to improve qualitative and quantitative production of this crop through basic tissue culture regeneration. Several attempts with various explants have already discovered in past but no initiative was reported for Bangladeshi sunflower varieties. In present study, optimum seed sterilization method; the efficiency of different explants; effect of different growth hormones in different combinations and concentrations and rooting efficiency of initiated shoots were demonstrated for two farmer popular Bangladeshi sunflower varieties, namely BARI Surjomukhi 2 and BRAC Hysun 33. The seeds were washed with 70% ethanol for 3 minutes followed by vigorous hand shaking with 14% Clorox for 20 minutes. To collect explant, named embryonic axis, testas were removed and 3 mm piece from proximal portion of the seed was excised and inoculated in different combinations and concentrations of hormone supplemented media. For explant cotyledon, sterilized seeds were inoculated in germination media to obtain different ages seedlings, thus five, seven and nine days cotyledon explant was collected. In case of explant embryonic axis, BRAC Hysun 33 variety showed maximum (58.33%) regeneration in 1.0 mg/l BAP with 0.1 mg/l NAA hormone supplemented media while in BARI Surjomukhi 2 variety, 2.0 mg/l BAP gave highest (46.67%) regeneration. Elongated shoots were inoculated in 0.2 mg/l IBA containing media for root induction but further studies are needed to optimize rooting media concentration. The second explant, cotyledon remained nonregenerative in both varieties. Further studies need to be done to develop regeneration system from this explant. In future reproducibility of this protocol using embryonic axis needs to be evaluated. Maisha Mosharrat Chowdhury B. Biotechnology 2015-05-12T06:29:25Z 2015-05-12T06:29:25Z 2015 2015-03 Thesis ID 11136010 http://hdl.handle.net/10361/4129 en BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 51 pages application/pdf BRAC University
institution Brac University
collection Institutional Repository
language English
topic Biotechnology
spellingShingle Biotechnology
Chowdhury, Maisha Mosharrat
In vitro culture establishment from Cotyledon and Embryonic Axis Explants of two Bangladeshi Sunflower (Helianthus annus L.) varieties
description This thesis report is submitted in partial fulfillment of the requirement for the degree of Bachelor of Science in Biotechnology, 2015.
author2 Islam, Aparna
author_facet Islam, Aparna
Chowdhury, Maisha Mosharrat
format Thesis
author Chowdhury, Maisha Mosharrat
author_sort Chowdhury, Maisha Mosharrat
title In vitro culture establishment from Cotyledon and Embryonic Axis Explants of two Bangladeshi Sunflower (Helianthus annus L.) varieties
title_short In vitro culture establishment from Cotyledon and Embryonic Axis Explants of two Bangladeshi Sunflower (Helianthus annus L.) varieties
title_full In vitro culture establishment from Cotyledon and Embryonic Axis Explants of two Bangladeshi Sunflower (Helianthus annus L.) varieties
title_fullStr In vitro culture establishment from Cotyledon and Embryonic Axis Explants of two Bangladeshi Sunflower (Helianthus annus L.) varieties
title_full_unstemmed In vitro culture establishment from Cotyledon and Embryonic Axis Explants of two Bangladeshi Sunflower (Helianthus annus L.) varieties
title_sort in vitro culture establishment from cotyledon and embryonic axis explants of two bangladeshi sunflower (helianthus annus l.) varieties
publisher BRAC University
publishDate 2015
url http://hdl.handle.net/10361/4129
work_keys_str_mv AT chowdhurymaishamosharrat invitrocultureestablishmentfromcotyledonandembryonicaxisexplantsoftwobangladeshisunflowerhelianthusannuslvarieties
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