IN VITRO culture establishment from hypocotyl and cotyledon explants of two Bangladeshi sunflower (Helianthus annuus L. ) varieties
This thesis report is submitted in partial fulfillment of the requirement for the degree of Bachelor of Science in Biotechnology, 2015.
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BRAC University
2015
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| Acceso en línea: | http://hdl.handle.net/10361/4103 |
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10361-41032019-09-30T04:12:15Z IN VITRO culture establishment from hypocotyl and cotyledon explants of two Bangladeshi sunflower (Helianthus annuus L. ) varieties Oshin, Afsana Tasnim Islam, Dr. Aparna Department of Mathematical and Natural Science, BRAC University Biotechnology Sunflower This thesis report is submitted in partial fulfillment of the requirement for the degree of Bachelor of Science in Biotechnology, 2015. Cataloged from PDF version of thesis report. Includes bibliographical references (page 43-46). Sunflower (Helianthus annuus.L) is the world’s second most important oilseed crop. Plant tissue culture and gene transformation are the important tools for improvement of this crop. The purposes of this study are to optimize seed sterilization procedure, determine the most suitable and efficient explants along with their suitable age and size, and to optimize the hormonal concentration for shoot regeneration. Seeds being treated with 70% ethanol for 3 minutes and 14% Clorox for 20 minutes, with their testa being removed before being inoculated into the germination medium, were found to be optimum for the tissue culture technique. In this study, two varieties, BARI surjomukhi 2 and BRAC Hysun 33 were used as plant materials. Hypocotyls and cotyledons were excised from 5, 7 and 9 days and cultured on MS medium supplemented with eight different combinations of BAP and NAA hormones. The experiments were kept in 18/6 hour light/dark photoperiod at 26±2ºC. It was determined that MS medium supplemented with 1 mg/l BAP and 0.1 mg/l NAA is the best combination for 7 day old hypocotyl explants of both BARI surjomukhi-2 and BRAC Hysun 33 varieties. From 7 day old hypocotyl explants, the best shoot regeneration response of BARI surjomukhi-2 and BRAC Hysun 33 variety was 60% and 53.3%, with the best grown shoot that grew up to 2.08 cm and a of length 1.61 cm within 30 days of regeneration respectively. This clearly showed that MS medium supplemented with 1 mg/l BAP and 0.1 mg/l NAA is the best hormonal media combination for hypocotyl explants. Shoot regeneration efficiencies of the cotyledon explants on the same medium were lower in comparison with the hypocotyl explants. Cotyledon explants only grew large in size with time, but did not show any positive response like shoot regeneration. So, hypocotyls were proved to be better explants than cotyledons. In future, rooting at the base of the regenerated shoots will be tried to generate whole plantlet. Afsana Tasnim Oshin B. Biotechnology 2015-04-16T07:03:00Z 2015-04-16T07:03:00Z 2015 2015-03 Thesis ID 11136003 http://hdl.handle.net/10361/4103 en BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 46 pages application/pdf BRAC University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Biotechnology Sunflower |
| spellingShingle |
Biotechnology Sunflower Oshin, Afsana Tasnim IN VITRO culture establishment from hypocotyl and cotyledon explants of two Bangladeshi sunflower (Helianthus annuus L. ) varieties |
| description |
This thesis report is submitted in partial fulfillment of the requirement for the degree of Bachelor of Science in Biotechnology, 2015. |
| author2 |
Islam, Dr. Aparna |
| author_facet |
Islam, Dr. Aparna Oshin, Afsana Tasnim |
| format |
Thesis |
| author |
Oshin, Afsana Tasnim |
| author_sort |
Oshin, Afsana Tasnim |
| title |
IN VITRO culture establishment from hypocotyl and cotyledon explants of two Bangladeshi sunflower (Helianthus annuus L. ) varieties |
| title_short |
IN VITRO culture establishment from hypocotyl and cotyledon explants of two Bangladeshi sunflower (Helianthus annuus L. ) varieties |
| title_full |
IN VITRO culture establishment from hypocotyl and cotyledon explants of two Bangladeshi sunflower (Helianthus annuus L. ) varieties |
| title_fullStr |
IN VITRO culture establishment from hypocotyl and cotyledon explants of two Bangladeshi sunflower (Helianthus annuus L. ) varieties |
| title_full_unstemmed |
IN VITRO culture establishment from hypocotyl and cotyledon explants of two Bangladeshi sunflower (Helianthus annuus L. ) varieties |
| title_sort |
in vitro culture establishment from hypocotyl and cotyledon explants of two bangladeshi sunflower (helianthus annuus l. ) varieties |
| publisher |
BRAC University |
| publishDate |
2015 |
| url |
http://hdl.handle.net/10361/4103 |
| work_keys_str_mv |
AT oshinafsanatasnim invitrocultureestablishmentfromhypocotylandcotyledonexplantsoftwobangladeshisunflowerhelianthusannuuslvarieties |
| _version_ |
1814308512008765440 |