Evaluation of helicobacter pyloristool antigen detection test
This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2014.
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| Ձևաչափ: | Թեզիս |
| Լեզու: | English |
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BRAC University
2014
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| Առցանց հասանելիություն: | http://hdl.handle.net/10361/3845 |
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10361-3845 |
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Brac University |
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Institutional Repository |
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English |
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Biotechnology Helicobacter pylori |
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Biotechnology Helicobacter pylori Khatun, Salma Evaluation of helicobacter pyloristool antigen detection test |
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This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2014. |
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Choudhury, Professor Dr. Naiyyum |
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Choudhury, Professor Dr. Naiyyum Khatun, Salma |
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Thesis |
| author |
Khatun, Salma |
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Khatun, Salma |
| title |
Evaluation of helicobacter pyloristool antigen detection test |
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Evaluation of helicobacter pyloristool antigen detection test |
| title_full |
Evaluation of helicobacter pyloristool antigen detection test |
| title_fullStr |
Evaluation of helicobacter pyloristool antigen detection test |
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Evaluation of helicobacter pyloristool antigen detection test |
| title_sort |
evaluation of helicobacter pyloristool antigen detection test |
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BRAC University |
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2014 |
| url |
http://hdl.handle.net/10361/3845 |
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AT khatunsalma evaluationofhelicobacterpyloristoolantigendetectiontest |
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1814307756680675328 |
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10361-38452019-09-30T04:22:13Z Evaluation of helicobacter pyloristool antigen detection test Khatun, Salma Choudhury, Professor Dr. Naiyyum Haq, Dr. Jalaluddin Ashraful Department of Mathematical and Natural Science, BRAC University Biotechnology Helicobacter pylori This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2014. Cataloged from PDF version of thesis report. Includes bibliographical references (page 50 - 83). Helicobacter pylori (H. pylori) is a human pathogen causing chronic gastritis, gastric and duodenal ulcer, gastric carcinoma and dyspepsia. The diagnosis of H. pylori is an essential element in the management of these gastrointestinal pathologies. There are several invasive and noninvasive methods for diagnosis of Helicobacter pylori infection. The plethora of diagnostic tests available for the presence of H. pylori indicates that none of them is 100% accurate. Invasive methods requiring endoscopic evaluation include bacteriologic culture, histopathological studies, smear examination, rapid urease test or CLO test and molecular methods. Noninvasive approaches include serological testing, stool antigen detection and urea breath testing. The stool antigen test is convenient, non-invasive, and does not require the use of expensive equipment or medical personnel. Furthermore, stool can be collected in a patient's home and transferred to the hospital or laboratory. The aim of the present study was to evaluate the efficacy of H. pylori stool antigen detection test (HpSA) in comparison with RUT and serological test. The present study was carried out on 92 adult patients suffering from dyspepsia and stool samples were examined from another 31 infants as control. From the patients, gastric biopsy specimens were taken for detection of H. pylori infection by rapid urease test (RUT). In addition, stool samples were taken for the detection of H. pylori antigen and blood samples for detection of anti- H. pylori IgA and IgG antibodies. Among 92 adult patients undergone endoscopic examination, 19 were found having ulcer and 73 were found having erosion. Out of 19 ulcer cases, RUT positive cases were 10 (52.63%) and among 73 erosion cases, 30 (41.09%) cases were RUT positive. H. pylori infection was detected in total 40 (43.48%) cases by RUT. Among 19 ulcer cases, 14 (73.68%) cases were positive for HpSA and 44 (60.27%) cases were positive for HpSA out of 73 erosion cases. A total 58 (63.04%) cases were HpSA positive with a sensitivity and specificity of 100% and 96.77% respectively. Serum anti H. pylori IgA and IgG antibodies were present in 58 (63.04%) and 89 (96.74%) cases respectively. Both antibodies were present in 57 (61.96%) cases. Among 40 RUT positive cases, IgG and IgA positive cases were 39 (97.5%) and 26 (65%) respectively and both antibodies were present in 25 (62.5%) cases. IgG and IgA positive cases were 50 (96.15%) and 32 (61.54%) respectively and both antibodies were present in 31 (59.62%) cases out of 52 RUT negative patients. Of 40 XIII RUT positive cases, 39 (97.5%) were stool Ag positive. Among 52 RUT negative cases, 19 (36.53%) were positive for stool Ag. Among 58 stool Ag positive patients, 57 (98.27%) cases were IgG positive and 39 (67.24%) cases were IgA positive and 38 (65.52%) cases were positive for both antibodies. Out of 34 stool Ag negative cases, 32 (94.11%) and 19 (55.88%) cases were positive for IgG and IgA respectively and both antibodies were present in 19 (55.88%) cases. Among 31 infants stool samples examined for H. pylori antigen as control, 30 (96.77%) were negative for stool Ag. In 58 stool Ag positive cases, serum IgG and IgA mean titre were (2.09 ± 0.46) and (1.40 ± 1.01) respectively. IgG mean titre was 1.94 ± 0.52 and IgA mean tire was 1.23 ± 0.95 in 34 stoo Ag negative patients. H. pylori stool antigen assay (HpSA) gave the highest rate of detection (63.04%), followed by serum antibody detection (61.96 %) and the lowest by RUT (43.48%). H. pylori stool antigen assay (HpSA) could be used as a routine diagnostic tool for H. pylori infection. Salma Khatun M. Biotechnology 2014-12-07T11:10:29Z 2014-12-07T11:10:29Z 2014 2014-11 Thesis ID 11376009 http://hdl.handle.net/10361/3845 en BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 90 pages application/pdf BRAC University |