Study on SOD1 gene +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin
This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2014.
| Autor principal: | |
|---|---|
| Altres autors: | |
| Format: | Thesis |
| Idioma: | English |
| Publicat: |
BRAC University
2014
|
| Matèries: | |
| Accés en línia: | http://hdl.handle.net/10361/3644 |
| id |
10361-3644 |
|---|---|
| record_format |
dspace |
| spelling |
10361-36442019-09-30T04:21:23Z Study on SOD1 gene +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin Shahriar, S.M. Choudhury, Prof. Naiyyum Ali, Prof. Liaquat Department of Mathematical and Natural Science, BRAC University Biotechnology Superoxide dismutase 1 (SOD1) This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2014. Cataloged from PDF version of thesis report. Includes bibliographical references (page 65-71). Superoxide dismutase 1 (SOD1), located at the cytosol has been postulated to represent between 50%-80% of the total SOD activity. The SOD gene is located at 21q22.1. Polymorphisms in the genes encoding these enzymes found to be significantly affecting its antioxidant role. As oxidative stress is a common pathogenic factor for the dysfunction of beta and endothelial cells, polymorphism of SOD genes and oxidative stress has become a subject of intense scrutiny for their association with different disease conditions including T2DM. The study was aimed to assess the possible association of SOD1 gene +35A/C polymorphism in the pathogenesis of IGR subjects of Bangladeshi origin. A total number of 54 middle aged (age range: 30-65year) subjects with Impaired Glucose Regulation (IGR) were consecutively recruited. Total number of healthy control was 55. IGR was diagnosed following WHO guideline. Blood glucose was measured by glucose-oxidase and, triglyceride, total cholesterol and HDL-cholesterol by enzymatic colorimetric method. Insulin was estimated by enzyme linked immunosorbent assay (ELISA). DNA was extracted using QIAGEN Blood DNA Kit which utilizes silica gel DNA separation. SOD1 gene +35A/C variant was determined by PCR-RFLP using restriction endonuclease HhaI. Data were managed using Statistical Program for Social Science (SPSS). Unpaired Student's -`t' test and Chi-squared tests, as appropriate, were performed. The number of study subjects of wild AA genotype in control and IGR group was 52 and 54 respectively whereas in case of heterozygous (Ht) variant AC genotype the number was 0 and 2 respectively. SOD1 +35A>C genotype frequency analysis has not shown statistical significant association (χ2 = 2.075 and p= 0.150). For control group, the genotype frequencies of AA and AC were 1.0 and 0 respectively. For IGR group, the frequencies were 0.963 and 0.037 respectively. The data showed that SOD1 gene +35A>C variant is substantially different from other population and the polymorphic allele is not associated with insulin secretory defect of IGR subjects of Bangladeshi origin. S. M. Shahriar M. Biotechnology 2014-09-16T07:12:11Z 2014-09-16T07:12:11Z 2014 2014-07 Thesis ID 12176007 http://hdl.handle.net/10361/3644 en BRAC University Thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 75 pages application/pdf BRAC University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Biotechnology Superoxide dismutase 1 (SOD1) |
| spellingShingle |
Biotechnology Superoxide dismutase 1 (SOD1) Shahriar, S.M. Study on SOD1 gene +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin |
| description |
This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2014. |
| author2 |
Choudhury, Prof. Naiyyum |
| author_facet |
Choudhury, Prof. Naiyyum Shahriar, S.M. |
| format |
Thesis |
| author |
Shahriar, S.M. |
| author_sort |
Shahriar, S.M. |
| title |
Study on SOD1 gene +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin |
| title_short |
Study on SOD1 gene +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin |
| title_full |
Study on SOD1 gene +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin |
| title_fullStr |
Study on SOD1 gene +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin |
| title_full_unstemmed |
Study on SOD1 gene +35A>C polymorphism in impaired glucose regulation patients of Bangladeshi origin |
| title_sort |
study on sod1 gene +35a>c polymorphism in impaired glucose regulation patients of bangladeshi origin |
| publisher |
BRAC University |
| publishDate |
2014 |
| url |
http://hdl.handle.net/10361/3644 |
| work_keys_str_mv |
AT shahriarsm studyonsod1gene35acpolymorphisminimpairedglucoseregulationpatientsofbangladeshiorigin |
| _version_ |
1814308032823164928 |