Analysis of factors influencing successful Agrobacterium-mediated genetic transformation in two Bangladeshi peanut (Arachis hypogaea L.) varieties BINA Chinabadam-2 and BINA Chinabadam-6 using embryonic leaflet explant

Analysis of factors influencing successful Agrobacterium-mediated genetic transformation in two Bangladeshi peanut (Arachis hypogaea L.) varieties BINA Chinabadam-2 and BINA Chinabadam-6 using embryonic leaflet explant

A thesis submitted to BRAC University in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology

Détails bibliographiques
Auteur principal: Chowdhury, Iftekhar Mahmud
Autres auteurs: Islam, Dr. Aparna
Format: Thèse
Langue:English
Publié: BRAC University 2014
Sujets:
Biotechnology
Accès en ligne:http://hdl.handle.net/10361/3052
id 10361-3052
record_format dspace
spelling 10361-30522019-09-30T04:12:11Z Analysis of factors influencing successful Agrobacterium-mediated genetic transformation in two Bangladeshi peanut (Arachis hypogaea L.) varieties BINA Chinabadam-2 and BINA Chinabadam-6 using embryonic leaflet explant Chowdhury, Iftekhar Mahmud Islam, Dr. Aparna Department of Mathematical and Natural Science, BRAC University Biotechnology A thesis submitted to BRAC University in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology Cataloged from PDF version of thesis report. Includes bibliographical references (page 25-27). Peanut (Arachis hypogaea L.) is an important crop both for its nutritional value and for its oil content in the seeds. But this economically important crop is susceptible to both biotic and abiotic factors that hamper its growth both in quality and quantity. Due to change in agricultural environment and also due to lack of resistance towards diseases the production is compromised. For this reason, genetic engineering is a possible tool to enhance the quality of the crop by introducing novel trait in it. To achieve that it a prerequisite to established a transformation protocol prior to any transformation event. Establishment of an efficient Agrobacterium-mediated transformation protocol requires optimization of several factors that affects the transfer of T-DNA. In the present study, these were optimized for two peanut varieties, namely, BINA Chinabadam 2, and BINA Chinabadam 6 using embryonic leaflet as a source of explant. In this study, Agrobacterium tumifaciens strain LBA4404 carrying pBI121 having GUS and nptII marker genes was used. T-DNA transfer was monitored through transient transformation assay utilizing GUS gene expression. Bacterial suspension having density (OD600) more than 1.0 with at least 1 hour of incubation period and minimum of 2 days of co-cultivation period found to be efficient for leaflet explants in both the varieties. Following transfer of T-DNA, presence of transgene within the infected tissue was observed by GUS histochemical transient assay. Iftekhar Mahmud Chowdhury B. Biotechnology 2014-03-18T08:16:24Z 2014-03-18T08:16:24Z 2014 2014-03 Thesis ID 10336001 http://hdl.handle.net/10361/3052 en BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 27 pages application/pdf BRAC University
institution Brac University
collection Institutional Repository
language English
topic Biotechnology
spellingShingle Biotechnology
Chowdhury, Iftekhar Mahmud
Analysis of factors influencing successful Agrobacterium-mediated genetic transformation in two Bangladeshi peanut (Arachis hypogaea L.) varieties BINA Chinabadam-2 and BINA Chinabadam-6 using embryonic leaflet explant
description A thesis submitted to BRAC University in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology
author2 Islam, Dr. Aparna
author_facet Islam, Dr. Aparna
Chowdhury, Iftekhar Mahmud
format Thesis
author Chowdhury, Iftekhar Mahmud
author_sort Chowdhury, Iftekhar Mahmud
title Analysis of factors influencing successful Agrobacterium-mediated genetic transformation in two Bangladeshi peanut (Arachis hypogaea L.) varieties BINA Chinabadam-2 and BINA Chinabadam-6 using embryonic leaflet explant
title_short Analysis of factors influencing successful Agrobacterium-mediated genetic transformation in two Bangladeshi peanut (Arachis hypogaea L.) varieties BINA Chinabadam-2 and BINA Chinabadam-6 using embryonic leaflet explant
title_full Analysis of factors influencing successful Agrobacterium-mediated genetic transformation in two Bangladeshi peanut (Arachis hypogaea L.) varieties BINA Chinabadam-2 and BINA Chinabadam-6 using embryonic leaflet explant
title_fullStr Analysis of factors influencing successful Agrobacterium-mediated genetic transformation in two Bangladeshi peanut (Arachis hypogaea L.) varieties BINA Chinabadam-2 and BINA Chinabadam-6 using embryonic leaflet explant
title_full_unstemmed Analysis of factors influencing successful Agrobacterium-mediated genetic transformation in two Bangladeshi peanut (Arachis hypogaea L.) varieties BINA Chinabadam-2 and BINA Chinabadam-6 using embryonic leaflet explant
title_sort analysis of factors influencing successful agrobacterium-mediated genetic transformation in two bangladeshi peanut (arachis hypogaea l.) varieties bina chinabadam-2 and bina chinabadam-6 using embryonic leaflet explant
publisher BRAC University
publishDate 2014
url http://hdl.handle.net/10361/3052
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