Isolation of Acinetobacter baumannii from patient and hospital environment: analyzing their antibiotic resistance, serum resistance & biofilm formation
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2024.
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Brac University
2024
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| גישה מקוונת: | http://hdl.handle.net/10361/24463 |
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10361-244632024-10-29T21:03:55Z Isolation of Acinetobacter baumannii from patient and hospital environment: analyzing their antibiotic resistance, serum resistance & biofilm formation Jinnah, Mst. Maskera Fatema, Kaniz Haque, Fahim Kabir Monjurul Department of Mathematics and Natural Sciences, Brac University Acinetobacter baumannii Multi-drug resistance Antibiotic resistance Serum resistance Biofilm formation Drug resistance in microorganisms. Biofilms--growth & development. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2024. Catalogued from PDF version of thesis. Includes bibliographical references (pages 35-39). The Acinetobacter baumannii is a gram-negative coccobacillus also known as opportunistic bacterial pathogens currently creating great concern in clinical aspects due to their capacity to endure for extended periods of time in the environment and ability to cause multi-drug resistant infections. The objective of the study was to identify A. baumannii from hospital environments and admitted patients in hospital to analyze their antibiotic resistance, serum resistance and biofilm formation. Total 450 samples were collected from different wards of Rajshahi Medical College and Hospital. Among them we obtained 53 isolates of A. baumannii confirmed by polymerase chain reaction by targeting of the blaOXA-51 gene. From 53 isolates, 20 were from patient’s samples (7 endotracheal aspirates, 4 blood, 5 wound swab, 2 throat swab and 2 catheter tube ) and 33 isolates were from environment’s samples (bed sheet, surface of furniture, nebulizer machine, floor, nurse’s hand swab, food cart, medicine cart and trolleys). Subsequently, an antibiotic susceptibility test was done. Isolates from patient specimens were resistant to gentamicin (90%), amikacin (90%), cefepime, piperacillin-tazobactam (85%), ceftazidime(85%), and tetracycline (80%). A significant proportion of the isolates, 70%, displayed resistant against levofloxacin, imipenem, meropenem. Most importantly, 90% of all patient isolates were MDR. On the other hand, hospital environment’s isolates were resistant against ceftazidime(100%), imipenem(87.9%,), piperacillin-tazobactam (78.8%), and cefepime(78.8%). A significant proportion of the isolates, 69.7% and 66.7% were resistant against meropenem and gentamicin. Among them 80% of all isolates were MDR. The result of the serum bactericidal assay showed that almost 31% of isolates were serum resistant and 35% were sensitive and 34% were intermediate. Isolates from the environment's samples were 10% more resistant than the isolates from the patient's samples. Among environment’s isolates 36% isolates were resistant whereas among patient’s isolates only 26% were resistant. According to quantitative biofilm formation results, among 33 environmental isolates 17% of isolates formed strong biofilm, 14% formed moderate film, and 30% formed weak and 39% isolates did not form biofilm. Mst. Maskera Jinnah Kaniz Fatema B.Sc. in Microbiology 2024-10-29T04:45:52Z 2024-10-29T04:45:52Z ©2024 2024-07 Thesis ID 19226010 ID 19126005 http://hdl.handle.net/10361/24463 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 40 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Acinetobacter baumannii Multi-drug resistance Antibiotic resistance Serum resistance Biofilm formation Drug resistance in microorganisms. Biofilms--growth & development. |
| spellingShingle |
Acinetobacter baumannii Multi-drug resistance Antibiotic resistance Serum resistance Biofilm formation Drug resistance in microorganisms. Biofilms--growth & development. Jinnah, Mst. Maskera Fatema, Kaniz Isolation of Acinetobacter baumannii from patient and hospital environment: analyzing their antibiotic resistance, serum resistance & biofilm formation |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2024. |
| author2 |
Haque, Fahim Kabir Monjurul |
| author_facet |
Haque, Fahim Kabir Monjurul Jinnah, Mst. Maskera Fatema, Kaniz |
| format |
Thesis |
| author |
Jinnah, Mst. Maskera Fatema, Kaniz |
| author_sort |
Jinnah, Mst. Maskera |
| title |
Isolation of Acinetobacter baumannii from patient and hospital environment: analyzing their antibiotic resistance, serum resistance & biofilm formation |
| title_short |
Isolation of Acinetobacter baumannii from patient and hospital environment: analyzing their antibiotic resistance, serum resistance & biofilm formation |
| title_full |
Isolation of Acinetobacter baumannii from patient and hospital environment: analyzing their antibiotic resistance, serum resistance & biofilm formation |
| title_fullStr |
Isolation of Acinetobacter baumannii from patient and hospital environment: analyzing their antibiotic resistance, serum resistance & biofilm formation |
| title_full_unstemmed |
Isolation of Acinetobacter baumannii from patient and hospital environment: analyzing their antibiotic resistance, serum resistance & biofilm formation |
| title_sort |
isolation of acinetobacter baumannii from patient and hospital environment: analyzing their antibiotic resistance, serum resistance & biofilm formation |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/24463 |
| work_keys_str_mv |
AT jinnahmstmaskera isolationofacinetobacterbaumanniifrompatientandhospitalenvironmentanalyzingtheirantibioticresistanceserumresistancebiofilmformation AT fatemakaniz isolationofacinetobacterbaumanniifrompatientandhospitalenvironmentanalyzingtheirantibioticresistanceserumresistancebiofilmformation |
| _version_ |
1814309160523661312 |