Analysis of lipase activity of JSF7 phage
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2022.
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| Формат: | Дисертація |
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Brac University
2024
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| Онлайн доступ: | http://hdl.handle.net/10361/24373 |
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10361-243732024-10-22T21:02:38Z Analysis of lipase activity of JSF7 phage Tabassum, Tasbiha Mohammad, Khondokar Shafin Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University JSF7 Rhodamine Fatty acids Lipase enzyme Lipases. Bacteriophages. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 62-63). The lytic phage of Vibrio cholera, JSF7 has been found to have a GDSL-like lipase enzyme in its tail region. The sequencing of the organism showed that in the ORF30 of the organism there is a coding region of this protein. In this research process, we have tried to find out the effectiveness of the lipase enzyme of this phage. The experiment was conducted on trial and error, and a dye of target Rhodamine B was used throughout the process. Before the experiment, it was found that there were certain bacterial strains of vibrio (WT 324 and WT 346) that are the host of this phage. We have used these hosts to enrich our phage from time to time and used the phages of high titer around 107/ml. The research was conducted using heating techniques to extract fatty acids from the oil as our control process and then using rhodamine on the sample to find out the efficiency of Rhodamine B. Using phages on the sample we tried to find out the correlation between our obtained results. After getting acceptable results we compared our results with the negative control of the experiment which was JSF2 which had already been sequenced and no lipase enzyme coding region was found. In the last part of the research, we conducted the experiment using the process of dose dependency where we used different concentrations of phage on the sample and measured their optical density (OD) using spectrophotometer. We found that samples with the highest concentration of phage were getting the maximum result. We found that samples with the highest concentration of phage were getting the maximum OD result due to more complexing of molecules of fatty acids and rhodamine B. Thus, this was hypothesized that more phage in the sample was causing the formation of more fatty acids. Tasbiha Tabassum Khondokar Shafin Mohammad B.Sc. in Biotechnology 2024-10-22T06:39:50Z 2024-10-22T06:39:50Z ©2022 2022-12 Thesis ID 18136027 ID 18136079 http://hdl.handle.net/10361/24373 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 63 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
JSF7 Rhodamine Fatty acids Lipase enzyme Lipases. Bacteriophages. |
| spellingShingle |
JSF7 Rhodamine Fatty acids Lipase enzyme Lipases. Bacteriophages. Tabassum, Tasbiha Mohammad, Khondokar Shafin Analysis of lipase activity of JSF7 phage |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2022. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Tabassum, Tasbiha Mohammad, Khondokar Shafin |
| format |
Thesis |
| author |
Tabassum, Tasbiha Mohammad, Khondokar Shafin |
| author_sort |
Tabassum, Tasbiha |
| title |
Analysis of lipase activity of JSF7 phage |
| title_short |
Analysis of lipase activity of JSF7 phage |
| title_full |
Analysis of lipase activity of JSF7 phage |
| title_fullStr |
Analysis of lipase activity of JSF7 phage |
| title_full_unstemmed |
Analysis of lipase activity of JSF7 phage |
| title_sort |
analysis of lipase activity of jsf7 phage |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/24373 |
| work_keys_str_mv |
AT tabassumtasbiha analysisoflipaseactivityofjsf7phage AT mohammadkhondokarshafin analysisoflipaseactivityofjsf7phage |
| _version_ |
1814308176925818880 |