Identification of Pseudomonas aeruginosa from post-traumatic wounds in Dhaka and their antimicrobial susceptibility profiles: a single-center cross-sectional study
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024.
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Brac University
2024
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| Sarrera elektronikoa: | http://hdl.handle.net/10361/24193 |
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10361-241932024-10-07T06:25:26Z Identification of Pseudomonas aeruginosa from post-traumatic wounds in Dhaka and their antimicrobial susceptibility profiles: a single-center cross-sectional study Drishti, Deblina Das Shithi, Alvia Amin Faria, Zareen Tasnim Haque, Fahim Kabir Monjurul Department of Mathematics and Natural Sciences, Brac University Post traumatic wounds Staphylococcus aureus Pseudomonas aeruginosa Polymerase chain reaction Antibiotic susceptibility Pseudomonas aeruginosa infections. Bacteria--Identification. Staphylococcus aureus infections. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2024. Catalogued from PDF version of thesis. Includes bibliographical references (pages 46-50). The purpose of the experimental study was to isolate the target organisms Pseudomonas aeruginosa and Staphylococcus aureus, from patients' wounds, which were generally recognized as post-traumatic wounds. All 45 patients were selected for post-traumatic wound cases, with 41 female and 4 male patients, and samples were taken from wound-infected areas. Next, the samples were cultured in two selective media cetrimide agar and mannitol salt agar (MSA) for these two specific organisms. After that, subculture was done on nutrient agar. Following this, DNA extraction was conducted in order to do the polymerase chain reaction (PCR) and agarose gel electrophoresis. Lastly, Kirby- Bauer Disc Diffusion method was used and CLSI guideline was followed for Antibiotic Susceptibility Test (AST). We found 18 isolates of Pseudomonas aeruginosa but no growth rate of the other targeted organism, Staphylococcus aureus, in 45 samples. Even though all the isolates from the patient group showed some percentage of resistance toward the antibiotics. Moreover, gram-negative isolates of the patient group exhibited high resistance to imipenem, gentamicin, cefepime, ampicillin and susceptible were seen against amikacin, meropenem, and ciprofloxacin. Furthermore, gram-negative Pseudomonas aeruginosa is mostly resistant to cephalosporins, aminoglycoside, and carbapenem group of antibiotics. The findings of this study highlight the significance of regular surveillance for patterns of antibiotic resistance in wound infections, specifically in cases of post-traumatic wounds. Deblina Das Drishti Alvia Amin Shithi Zareen Tasnim Faria B.Sc. in Biotechnology B.Sc. in Microbiology 2024-09-25T10:15:07Z 2024-09-25T10:15:07Z ©2024 2024-04 Thesis ID 20136044 ID 19336033 ID 18126065 http://hdl.handle.net/10361/24193 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 50 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Post traumatic wounds Staphylococcus aureus Pseudomonas aeruginosa Polymerase chain reaction Antibiotic susceptibility Pseudomonas aeruginosa infections. Bacteria--Identification. Staphylococcus aureus infections. |
| spellingShingle |
Post traumatic wounds Staphylococcus aureus Pseudomonas aeruginosa Polymerase chain reaction Antibiotic susceptibility Pseudomonas aeruginosa infections. Bacteria--Identification. Staphylococcus aureus infections. Drishti, Deblina Das Shithi, Alvia Amin Faria, Zareen Tasnim Identification of Pseudomonas aeruginosa from post-traumatic wounds in Dhaka and their antimicrobial susceptibility profiles: a single-center cross-sectional study |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024. |
| author2 |
Haque, Fahim Kabir Monjurul |
| author_facet |
Haque, Fahim Kabir Monjurul Drishti, Deblina Das Shithi, Alvia Amin Faria, Zareen Tasnim |
| format |
Thesis |
| author |
Drishti, Deblina Das Shithi, Alvia Amin Faria, Zareen Tasnim |
| author_sort |
Drishti, Deblina Das |
| title |
Identification of Pseudomonas aeruginosa from post-traumatic wounds in Dhaka and their antimicrobial susceptibility profiles: a single-center cross-sectional study |
| title_short |
Identification of Pseudomonas aeruginosa from post-traumatic wounds in Dhaka and their antimicrobial susceptibility profiles: a single-center cross-sectional study |
| title_full |
Identification of Pseudomonas aeruginosa from post-traumatic wounds in Dhaka and their antimicrobial susceptibility profiles: a single-center cross-sectional study |
| title_fullStr |
Identification of Pseudomonas aeruginosa from post-traumatic wounds in Dhaka and their antimicrobial susceptibility profiles: a single-center cross-sectional study |
| title_full_unstemmed |
Identification of Pseudomonas aeruginosa from post-traumatic wounds in Dhaka and their antimicrobial susceptibility profiles: a single-center cross-sectional study |
| title_sort |
identification of pseudomonas aeruginosa from post-traumatic wounds in dhaka and their antimicrobial susceptibility profiles: a single-center cross-sectional study |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/24193 |
| work_keys_str_mv |
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| _version_ |
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