Isolation, identification and characterization of plastic degrading Gut bacteria from Zophobas atratus larvae
This thesis is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2024.
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| Формат: | Дисертація |
| Мова: | English |
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Brac University
2024
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| Онлайн доступ: | http://hdl.handle.net/10361/24191 |
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10361-241912024-09-25T21:01:44Z Isolation, identification and characterization of plastic degrading Gut bacteria from Zophobas atratus larvae Zaman, Ifthikhar Hossain, M. Mahboob Department of Mathematics and Natural Sciences, Brac University Zophobas atratus Plastic pollution Linear low-density polyethylene Expanded polystyrene Pseudomonas aeruginosa Plastic scrap--Environmental aspects. Microbial ecology. Bacteria. This thesis is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2024. Cataloged from PDF version of thesis. Includes bibliographical references (pages 69-83). Plastic pollution has become a major environmental concern globally, and novel and eco-friendly approaches like bioremediation are essential to mitigate the impact. This study investigated the biodegradation of three common plastic types, LDPE, LLDPE, and EPS, by Zophobas atratus larvae. Over 36 days, the average larval consumption was found to be 24.04% LDPE, 20.01% EPS and 15.12% LLDPE. FTIR analysis confirmed plastic oxidation in the gut. Gut bacteria were selectively isolated and identified as Pseudomonas aeruginosa strains. These bacteria showed the ability to degrade specific plastic types confirmed by SEM. Whole genome sequencing revealed many enzymes, along with virulence factors, antibiotic-resistance genes, and rhamnolipid biosurfactant biosynthesis genes in both isolates. Rhamnolipid analysis and AST were performed. This study indicated Zophobas atratus larvae as potential LDPE, LLDPE, and EPS biodegradation agents. Additionally, the isolated strains of Pseudomonas aeruginosa provide a more direct and eco-friendly solution for plastic degradation. Ifthikhar Zaman M.Sc. in Biotechnology 2024-09-25T08:43:05Z 2024-09-25T08:43:05Z ©2024 2024-04 Thesis ID 23376006 http://hdl.handle.net/10361/24191 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 96 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Zophobas atratus Plastic pollution Linear low-density polyethylene Expanded polystyrene Pseudomonas aeruginosa Plastic scrap--Environmental aspects. Microbial ecology. Bacteria. |
| spellingShingle |
Zophobas atratus Plastic pollution Linear low-density polyethylene Expanded polystyrene Pseudomonas aeruginosa Plastic scrap--Environmental aspects. Microbial ecology. Bacteria. Zaman, Ifthikhar Isolation, identification and characterization of plastic degrading Gut bacteria from Zophobas atratus larvae |
| description |
This thesis is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2024. |
| author2 |
Hossain, M. Mahboob |
| author_facet |
Hossain, M. Mahboob Zaman, Ifthikhar |
| format |
Thesis |
| author |
Zaman, Ifthikhar |
| author_sort |
Zaman, Ifthikhar |
| title |
Isolation, identification and characterization of plastic degrading Gut bacteria from Zophobas atratus larvae |
| title_short |
Isolation, identification and characterization of plastic degrading Gut bacteria from Zophobas atratus larvae |
| title_full |
Isolation, identification and characterization of plastic degrading Gut bacteria from Zophobas atratus larvae |
| title_fullStr |
Isolation, identification and characterization of plastic degrading Gut bacteria from Zophobas atratus larvae |
| title_full_unstemmed |
Isolation, identification and characterization of plastic degrading Gut bacteria from Zophobas atratus larvae |
| title_sort |
isolation, identification and characterization of plastic degrading gut bacteria from zophobas atratus larvae |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/24191 |
| work_keys_str_mv |
AT zamanifthikhar isolationidentificationandcharacterizationofplasticdegradinggutbacteriafromzophobasatratuslarvae |
| _version_ |
1814307679126945792 |