The prevalence of multi-drug-resistant staphylococcus aureus and klebsiella pneumoniae in cooked meat samples collected from hospital cafeterias in the Dhaka city corporation area
This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024.
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Brac University
2024
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| On-line přístup: | http://hdl.handle.net/10361/24172 |
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10361-241722024-09-24T21:00:29Z The prevalence of multi-drug-resistant staphylococcus aureus and klebsiella pneumoniae in cooked meat samples collected from hospital cafeterias in the Dhaka city corporation area Hoque, Mahmuda Mesbah, Afrina Bala, Anik Shuvro Khan, Kashmery Department of Mathematics and Natural Sciences, Brac University Foodborne diseases Klebsiella pneumoniae Staphylococcus aureus Drug resistance Drug resistance in microorganisms. This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024. Cataloged from PDF version of thesis. Includes bibliographical references (pages 73-82). Foodborne diseases are a common threat in Bangladesh since study showed that 30 million people suffer from food poisoning in our country annually. One of the most favorite and nutritious food items in Bangladesh is chicken curry. Foodborne pathogens present in cooked chicken curry have not been investigated in Bangladesh yet. On the other hand, there have been several food poisoning outbreaks in various nations like Italy and South Korea due to Staphylococcus aureus and Klebsiella pneumoniae contamination in food. The study aims to investigate the prevalence of multi-drug resistant Staphylococcus aureus and Klebsiella pneumoniae from cooked meat collected from government hospital cafeterias in Dhaka city. In the current experiment, samples were collected from 9 hospitals covering five prime locations in Dhaka city. The study was conducted from April 2023 to March 2024. Presumptive isolates of Staphylococcus aureus and Klebsiella pneumoniae through the usage of selective and differential media named Mannitol Salt Agar (MSA) and Eosin methylene blue agar (EMB) and biochemical tests- (i) Gram staining, (ii) Triple Sugar Iron test. (iii) Citrate Utilization test. (iv) Catalase test and (v) Oxidase Test.Out of 63 presumptive Staphylococcus aureus isolates based on MSA media, 49 were positive based on biochemical tests. Molecular detection of the presumptive isolates of Staphylococcus aureus and Klebsiella pneumoniae was carried out using polymerase chain reaction (PCR) using nucA and ITS sequence primers, respectively. Out of 49 presumptive Staphylococcus aureus isolates based on biochemical tests, 14.3% were confirmed positive isolates of Staphylococcus aureus through PCR and Gel Electrophoresis. All the Staphylococcus aureus isolates were multidrug-resistant (MDR), and among them, 57.1% are Methicillin-resistant Staphylococcus aureus (MRSA). Out of 14 isolates of presumptive Klebsiella pneumoniae isolates based on EMB media, all the isolates were positive for biochemical tests. Molecular detection also confirmed the presence of Klebsiella pneumoniae. Interestingly, this isolate was multi-drug resistant to ampicillin, Ceftazidime, and Colistin antibiotics. Mahmuda Hoque Afrina Mesbah Anik Shuvro Bala B. Biotechnology 2024-09-24T05:38:49Z 2024-09-24T05:38:49Z 2024 2024-05 Thesis ID: 19336021 ID: 19336026 ID: 19336030 http://hdl.handle.net/10361/24172 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 82 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Foodborne diseases Klebsiella pneumoniae Staphylococcus aureus Drug resistance Drug resistance in microorganisms. |
| spellingShingle |
Foodborne diseases Klebsiella pneumoniae Staphylococcus aureus Drug resistance Drug resistance in microorganisms. Hoque, Mahmuda Mesbah, Afrina Bala, Anik Shuvro The prevalence of multi-drug-resistant staphylococcus aureus and klebsiella pneumoniae in cooked meat samples collected from hospital cafeterias in the Dhaka city corporation area |
| description |
This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024. |
| author2 |
Khan, Kashmery |
| author_facet |
Khan, Kashmery Hoque, Mahmuda Mesbah, Afrina Bala, Anik Shuvro |
| format |
Thesis |
| author |
Hoque, Mahmuda Mesbah, Afrina Bala, Anik Shuvro |
| author_sort |
Hoque, Mahmuda |
| title |
The prevalence of multi-drug-resistant staphylococcus aureus and klebsiella pneumoniae in cooked meat samples collected from hospital cafeterias in the Dhaka city corporation area |
| title_short |
The prevalence of multi-drug-resistant staphylococcus aureus and klebsiella pneumoniae in cooked meat samples collected from hospital cafeterias in the Dhaka city corporation area |
| title_full |
The prevalence of multi-drug-resistant staphylococcus aureus and klebsiella pneumoniae in cooked meat samples collected from hospital cafeterias in the Dhaka city corporation area |
| title_fullStr |
The prevalence of multi-drug-resistant staphylococcus aureus and klebsiella pneumoniae in cooked meat samples collected from hospital cafeterias in the Dhaka city corporation area |
| title_full_unstemmed |
The prevalence of multi-drug-resistant staphylococcus aureus and klebsiella pneumoniae in cooked meat samples collected from hospital cafeterias in the Dhaka city corporation area |
| title_sort |
prevalence of multi-drug-resistant staphylococcus aureus and klebsiella pneumoniae in cooked meat samples collected from hospital cafeterias in the dhaka city corporation area |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/24172 |
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