Association of MTHFR rs1801133, PEMT rs4244593, CHKA rs7928739, VNTRs IL-1RN intron 2 and NOS3 intron 4 polymorphisms with unexplained intrauterine fetal death in Bangladeshi population
This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology,2024.
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Brac University
2024
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| Acceso en liña: | http://hdl.handle.net/10361/24045 |
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10361-240452024-09-10T21:03:27Z Association of MTHFR rs1801133, PEMT rs4244593, CHKA rs7928739, VNTRs IL-1RN intron 2 and NOS3 intron 4 polymorphisms with unexplained intrauterine fetal death in Bangladeshi population Mahasin, Deera Bushra, Luthfa Akter Nusrat, Shifat E Hossain, Mohammed Mahboob Kabir, Yearul Department of Mathematics and Natural Sciences, Brac University. Polymorphisms Fetal death DNA repair genes Biomarker Fetal death. Polymorphism--Genetics. This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology,2024. Cataloged from PDF version of thesis. Includes bibliographical references (pages 73-82). This case-control study aimed to analyze interaction between increased risk of Intrauterine Fetal Death in Bangladeshi pregnant women and Single Nucleotide Polymorphism (SNP) of MTHFR (rs1801133), PEMT (rs4244593), CHKA (rs7928739), IL-1RN (intron 2 VNTR), and NOS3 (intron 4 VNTR). This study comprised 62 cases of IUFD and 64 controls, employed PCR/RFLP technique for MTHFR, PEMT, and CHKA gene, and only real-time PCR for IL1RN and NOS3 to observe repeat numbers. Compared to homozygous wild type (C/C) variant, heterozygous (C/A) and homozygous mutant (A/A) genotypes have shown increased risk (OR=3.18; 95% CI–1.02-9.91; p=0.04; OR=3.30; 95% Cl=0.98–11.07; p=0.05) of IUFD in PEMT analysis. However, the PEMT C/A and A/A genotypes were found to be associated with IUFD risk, in cases with no previous use of birth control (OR=0.27; 95% CI=0.08-0.92; p=0.04 and OR=0.02; 95% CI=0.06–0.79; p=0.02 respectively). IL1RN (Allele b/b) exhibited increased risk for IUFD when interacted with PEMT (A/A) genotype by 2 folds (OR=2; 95% CI=0.15–26.73; p=0.05). The genotypes of CHKA did not show any significant increase in IUFD risk. Interestingly, MTHFR and NOS3 manifested uniform distribution among cases and controls suggesting no association with IUFD risk in the Bangladeshi population. Deera Mahasin Luthfa Akter Bushra Shifat E Nusrat B. Biotechnology 2024-09-10T09:24:55Z 2024-09-10T09:24:55Z ©2024 2024-04 Thesis ID 20136037 ID 20136017 ID 20136038 http://hdl.handle.net/10361/24045 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 83 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Polymorphisms Fetal death DNA repair genes Biomarker Fetal death. Polymorphism--Genetics. |
| spellingShingle |
Polymorphisms Fetal death DNA repair genes Biomarker Fetal death. Polymorphism--Genetics. Mahasin, Deera Bushra, Luthfa Akter Nusrat, Shifat E Association of MTHFR rs1801133, PEMT rs4244593, CHKA rs7928739, VNTRs IL-1RN intron 2 and NOS3 intron 4 polymorphisms with unexplained intrauterine fetal death in Bangladeshi population |
| description |
This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology,2024. |
| author2 |
Hossain, Mohammed Mahboob |
| author_facet |
Hossain, Mohammed Mahboob Mahasin, Deera Bushra, Luthfa Akter Nusrat, Shifat E |
| format |
Thesis |
| author |
Mahasin, Deera Bushra, Luthfa Akter Nusrat, Shifat E |
| author_sort |
Mahasin, Deera |
| title |
Association of MTHFR rs1801133, PEMT rs4244593, CHKA rs7928739, VNTRs IL-1RN intron 2 and NOS3 intron 4 polymorphisms with unexplained intrauterine fetal death in Bangladeshi population |
| title_short |
Association of MTHFR rs1801133, PEMT rs4244593, CHKA rs7928739, VNTRs IL-1RN intron 2 and NOS3 intron 4 polymorphisms with unexplained intrauterine fetal death in Bangladeshi population |
| title_full |
Association of MTHFR rs1801133, PEMT rs4244593, CHKA rs7928739, VNTRs IL-1RN intron 2 and NOS3 intron 4 polymorphisms with unexplained intrauterine fetal death in Bangladeshi population |
| title_fullStr |
Association of MTHFR rs1801133, PEMT rs4244593, CHKA rs7928739, VNTRs IL-1RN intron 2 and NOS3 intron 4 polymorphisms with unexplained intrauterine fetal death in Bangladeshi population |
| title_full_unstemmed |
Association of MTHFR rs1801133, PEMT rs4244593, CHKA rs7928739, VNTRs IL-1RN intron 2 and NOS3 intron 4 polymorphisms with unexplained intrauterine fetal death in Bangladeshi population |
| title_sort |
association of mthfr rs1801133, pemt rs4244593, chka rs7928739, vntrs il-1rn intron 2 and nos3 intron 4 polymorphisms with unexplained intrauterine fetal death in bangladeshi population |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/24045 |
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