Isolation and molecular identification of salt tolerant probiotic bacteria and their impacts on rice (Oryza sativa) seedlings
This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Master of Science in Biotechnology, 2022.
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Brac University
2024
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| Accès en ligne: | http://hdl.handle.net/10361/24025 |
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10361-240252024-09-09T21:03:23Z Isolation and molecular identification of salt tolerant probiotic bacteria and their impacts on rice (Oryza sativa) seedlings Rahman, Mahmud -Ur Islam, Md Tofazzal Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University. Soil salinity Probiotic Salinity stress Plant growth promotion Soils, Salts in. Salinity. Growth (Plants). This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Master of Science in Biotechnology, 2022. Cataloged from PDF version of thesis. Includes bibliographical references (pages 65-85). Soil salinity is a serious problem in the coastal areas of Bangladesh. It is the main barrier to attain sustainability in crop production in those particular areas. Moreover, the situation is getting worse along with time. Therefore, an eco-friendly and sustainable approach is required to overcome this problem. Discovery of plant growth promoting bacteria (PGPB) and their application to crop plants are considered as promising and effective biotechnological approaches to fight against salt-stress to crop plants. The objectives of this study were to isolate and characterize salt tolerant PGPB from the rice plants cultivated in saline soils and evaluate their performances on seed germination and seedling growth of rice. The study was performed at the laboratories of the Institution of Biotechnology and Genetic Engineering (IBGE) in Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU). Rice plant samples were collected from salt-affected areas in Chattogram, Noakhali, Lakshmipur and Cox’s Bazar districts. Forty one salinity tolerant bacteria were isolated and characterized for screening in vitro for both salinity tolerance, and three highly salt tolerant isolates were further evaluated on seed germination and seedling growth of rice cv. BRRI dhan 29 (salinity susceptible) and BINA dhan 10 (salinity tolerant). Priming of rice seeds with three highly salt tolerant (up to 12% NaCl w/v) isolate viz. BTCoSo2, BTCoR2 and IBGE3C promoting growth of rice seedling and the effects were pronounced in BINA dhan 10. Among the three bacteria, IBGE3C displayed best performances on seedling growth of rice under varying salinity was sequenced and the strain exhibited 100% 16S ribosomal RNA gene sequence homology with Brevibacterium sediminis IBGE3C (accession no. MZ573246) strain. Brevibacterium sediminis is a potential plant growth promoting bacteria and it can significantly increase the shoot and root length in plants. Also, the isolation of B. sediminis from sea water and deep-sea sediments has been previously reported v which suggests it as a potential salt tolerant bacteria. Brevibacterium spp. are rod- shaped, non-spore forming gram-positive bacteria. This study for the first time identified B. sediminis strain IBGE3C (accession no. MZ573246) as a salt tolerant PGPB from the rice cultivated in Lakshmipur district of Bangladesh. In addition, the collected rice variety was BRRI dhan 28 which is also a widely cultivated variety throughout the country. However, the bacteria has been isolated from plant’s root sample which is required for further study for its practical application in the enhancement rice production in the saline soils in Southern districts of Bangladesh. Mahmud -Ur- Rahman M. Biotechnology 2024-09-09T04:05:29Z 2024-09-09T04:05:29Z ©2022 2021-10 Thesis ID 18376009 http://hdl.handle.net/10361/24025 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 101 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Soil salinity Probiotic Salinity stress Plant growth promotion Soils, Salts in. Salinity. Growth (Plants). |
| spellingShingle |
Soil salinity Probiotic Salinity stress Plant growth promotion Soils, Salts in. Salinity. Growth (Plants). Rahman, Mahmud -Ur Isolation and molecular identification of salt tolerant probiotic bacteria and their impacts on rice (Oryza sativa) seedlings |
| description |
This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Master of Science in Biotechnology, 2022. |
| author2 |
Islam, Md Tofazzal |
| author_facet |
Islam, Md Tofazzal Rahman, Mahmud -Ur |
| format |
Thesis |
| author |
Rahman, Mahmud -Ur |
| author_sort |
Rahman, Mahmud -Ur |
| title |
Isolation and molecular identification of salt tolerant probiotic bacteria and their impacts on rice (Oryza sativa) seedlings |
| title_short |
Isolation and molecular identification of salt tolerant probiotic bacteria and their impacts on rice (Oryza sativa) seedlings |
| title_full |
Isolation and molecular identification of salt tolerant probiotic bacteria and their impacts on rice (Oryza sativa) seedlings |
| title_fullStr |
Isolation and molecular identification of salt tolerant probiotic bacteria and their impacts on rice (Oryza sativa) seedlings |
| title_full_unstemmed |
Isolation and molecular identification of salt tolerant probiotic bacteria and their impacts on rice (Oryza sativa) seedlings |
| title_sort |
isolation and molecular identification of salt tolerant probiotic bacteria and their impacts on rice (oryza sativa) seedlings |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/24025 |
| work_keys_str_mv |
AT rahmanmahmudur isolationandmolecularidentificationofsalttolerantprobioticbacteriaandtheirimpactsonriceoryzasativaseedlings |
| _version_ |
1814308775921713152 |