Genetic susceptibility to coronary artery disease in Bangladesh: evaluation of MTHFR gene polymorphisms (C677T and A1298C) and hyperhomocysteinemia as independent risk factors of coronary artery disease among young Bangladeshi adults
This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024.
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Brac University
2024
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10361-239992024-09-11T07:44:24Z Genetic susceptibility to coronary artery disease in Bangladesh: evaluation of MTHFR gene polymorphisms (C677T and A1298C) and hyperhomocysteinemia as independent risk factors of coronary artery disease among young Bangladeshi adults Alabbi, Mysha Nowrin Malek, Naila Masfiqua Nanziba, Rifah Department of Mathematics and Natural Sciences, Brac University Coronary artery disease Homocysteine Hyperhomocysteinemia MTHFR gene polymorphism Hyperhomocysteinemia. This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024. Cataloged from PDF version of thesis. Includes bibliographical references (pages 72-82). Coronary artery disease (CAD) remains a leading cause of morbidity and mortality worldwide. This study investigates the role of elevated plasma homocysteine (Hcy) levels as an independent risk factor for CAD and explores the association of MTHFR C677T and A1298C gene polymorphisms with onset of CAD in young Bangladeshi population (≤ 50 years). Blood samples and data from 60 CAD patients and 60 healthy controls were analyzed. Biochemical tests were used to measure plasma Hcy levels and other markers of CAD. PCR-RFLP was used for genotype analysis. Our results showed that 11.7% of CAD patients had elevated Hcy levels compared to 6.7% of controls (p = 0.53), indicating a marginally higher prevalence of hyperhomocysteinemia (Hhcy) among CAD patients, though this difference was not statistically significant. The genotype analysis showed 80 individuals with the CC genotype, 35 with CT genotype, and no TT genotype was observed. Genotype analysis identified significant associations between MTHFR genotypes and several risk factors: Hhcy and male gender (p = 0.0004), family history of cardiovascular disease (p = 0.0175), and hypertension (p = 0.0341). Furthermore, we found no significant associations between Hhcy and conventional cardiovascular risk factors, suggesting that Hhcy may contribute to CAD through mechanisms independent of traditional risk factors. Our study also uncovered critical dietary factors associated with CAD risk. Higher intake of sugar (OR = 15.129, 95% CI: 4.273–53.565, p < 0.001) and chicken (OR = 5.776, 95% CI: 1.287–25.923, p = 0.022) were linked to increased CAD risk, whereas frequent fish consumption (OR = 0.227, 95% CI: 0.063–0.816, p = 0.023) was associated with a reduced risk. These findings emphasize the importance of dietary modifications in the prevention of CAD. In conclusion, while our study highlights the potential role of Hhcy and specific dietary patterns in CAD risk, the lack of statistical significance in Hhcy's association with CAD suggests a complex interplay between factors. Mysha Nowrin Alabbi Rifah Nanziba Naila Masfiqua Malek B. Biotechnology 2024-09-08T05:05:51Z 2024-09-08T05:05:51Z ©2024 2024-06 Thesis ID 20136032 ID 20136026 ID 20136031 http://hdl.handle.net/10361/23999 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 82 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Coronary artery disease Homocysteine Hyperhomocysteinemia MTHFR gene polymorphism Hyperhomocysteinemia. |
| spellingShingle |
Coronary artery disease Homocysteine Hyperhomocysteinemia MTHFR gene polymorphism Hyperhomocysteinemia. Alabbi, Mysha Nowrin Malek, Naila Masfiqua Nanziba, Rifah Genetic susceptibility to coronary artery disease in Bangladesh: evaluation of MTHFR gene polymorphisms (C677T and A1298C) and hyperhomocysteinemia as independent risk factors of coronary artery disease among young Bangladeshi adults |
| description |
This thesis submitted to the Department of Mathematics and Natural Sciences in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024. |
| author2 |
Department of Mathematics and Natural Sciences, Brac University |
| author_facet |
Department of Mathematics and Natural Sciences, Brac University Alabbi, Mysha Nowrin Malek, Naila Masfiqua Nanziba, Rifah |
| format |
Thesis |
| author |
Alabbi, Mysha Nowrin Malek, Naila Masfiqua Nanziba, Rifah |
| author_sort |
Alabbi, Mysha Nowrin |
| title |
Genetic susceptibility to coronary artery disease in Bangladesh: evaluation of MTHFR gene polymorphisms (C677T and A1298C) and hyperhomocysteinemia as independent risk factors of coronary artery disease among young Bangladeshi adults |
| title_short |
Genetic susceptibility to coronary artery disease in Bangladesh: evaluation of MTHFR gene polymorphisms (C677T and A1298C) and hyperhomocysteinemia as independent risk factors of coronary artery disease among young Bangladeshi adults |
| title_full |
Genetic susceptibility to coronary artery disease in Bangladesh: evaluation of MTHFR gene polymorphisms (C677T and A1298C) and hyperhomocysteinemia as independent risk factors of coronary artery disease among young Bangladeshi adults |
| title_fullStr |
Genetic susceptibility to coronary artery disease in Bangladesh: evaluation of MTHFR gene polymorphisms (C677T and A1298C) and hyperhomocysteinemia as independent risk factors of coronary artery disease among young Bangladeshi adults |
| title_full_unstemmed |
Genetic susceptibility to coronary artery disease in Bangladesh: evaluation of MTHFR gene polymorphisms (C677T and A1298C) and hyperhomocysteinemia as independent risk factors of coronary artery disease among young Bangladeshi adults |
| title_sort |
genetic susceptibility to coronary artery disease in bangladesh: evaluation of mthfr gene polymorphisms (c677t and a1298c) and hyperhomocysteinemia as independent risk factors of coronary artery disease among young bangladeshi adults |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23999 |
| work_keys_str_mv |
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