Downregulation of SOCS1 gene in the development of Guillain-Barré syndrome
This thesis is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2023.
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Brac University
2024
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| オンライン･アクセス: | http://hdl.handle.net/10361/23749 |
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10361-237492024-08-13T21:02:14Z Downregulation of SOCS1 gene in the development of Guillain-Barré syndrome Khurshid, Sarah Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University GBS Suppressor of cytokine signaling SOCS mRNA expression SOCS1 Guillain-Barré syndrome JAK-STAT pathway This thesis is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2023. Cataloged from PDF version of thesis. Includes bibliographical references (pages 57-59). Guillain-Barre syndrome (GBS), is a post-infectious disorder of the peripheral nervous system mediated by an aberrant immune response where cytokines play an imperative role in disease pathogenesis. Suppressor of cytokine signaling (SOCS1) proteins, a classical inhibitor of the JAKSTAT signaling pathway, which regulates down-regulates cytokine signaling and suppresses autoimmunity. Therefore, we aimed to determine the association of SOCS1 protein gene expression with GBS immunopathogenesis. This prospective study included 33 GBS patients and 33 healthy controls (HC) in Bangladesh. Detailed clinical investigations and blood samples were collected. Expression of SOCS1 gene was determined by qPCR and measured by using the 2−ΔCT method. Mann-Whitney U-test was performed to measure statistical difference of the 2−ΔCT values among the case and controls. The Pearson correlation was performed to find the association of SOCS1 mRNA expression with the response of regulatory T cells (Tregs) in GBS and healthy individuals. The median age of patients was 31 years (IQR: 22–40), with male predominance (65%). 69% of patients had antecedent events, mostly diarrhea (60 %), and 33% were severely affected. We found significant downregulation of SOCS1 mRNA expression in GBS patients compared to HCs (median: 0.7582 vs. 1.198, P = 0.0463). We also found a statistically insignificant trend of decrease in the expression of SOCS1 mRNA in severe GBS patients compared to mild GBS (median=0.7582 vs. 2.370). However, there were no significant associations between SOCS1 gene expressions and other clinical manifestations of GBS. In addition to that, no significant correlation was found between SOCS1 mRNA expression and CD4+CD25+Tregs, CD4+FOXP3+Tregs, and CD4+CD25+FOXP3+Tregs cell responses in patients with GBS and HCs. Thus, downregulation of the SOCS1 gene may play a pivotal role in developing this autoimmunity in patients with GBS. A large sample size is required to validate the association of the SOCS1 gene with GBS pathogenesis and further clinical progression of the disease. Sarah Khurshid M. Biotechnology 2024-08-13T06:14:12Z 2024-08-13T06:14:12Z ©2023 2023-12 Thesis ID 21276002 http://hdl.handle.net/10361/23749 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 59 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
GBS Suppressor of cytokine signaling SOCS mRNA expression SOCS1 Guillain-Barré syndrome JAK-STAT pathway |
| spellingShingle |
GBS Suppressor of cytokine signaling SOCS mRNA expression SOCS1 Guillain-Barré syndrome JAK-STAT pathway Khurshid, Sarah Downregulation of SOCS1 gene in the development of Guillain-Barré syndrome |
| description |
This thesis is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2023. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Khurshid, Sarah |
| format |
Thesis |
| author |
Khurshid, Sarah |
| author_sort |
Khurshid, Sarah |
| title |
Downregulation of SOCS1 gene in the development of Guillain-Barré syndrome |
| title_short |
Downregulation of SOCS1 gene in the development of Guillain-Barré syndrome |
| title_full |
Downregulation of SOCS1 gene in the development of Guillain-Barré syndrome |
| title_fullStr |
Downregulation of SOCS1 gene in the development of Guillain-Barré syndrome |
| title_full_unstemmed |
Downregulation of SOCS1 gene in the development of Guillain-Barré syndrome |
| title_sort |
downregulation of socs1 gene in the development of guillain-barré syndrome |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23749 |
| work_keys_str_mv |
AT khurshidsarah downregulationofsocs1geneinthedevelopmentofguillainbarresyndrome |
| _version_ |
1814308096942538752 |