Detection of Extended Spectrum β-lactamase (ESBL) And Carbapenemase Encoding Escherichia coli isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology and Bachelor of Science in Microbiology, 2023.
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Brac University
2024
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10361-235642024-06-25T21:03:48Z Detection of Extended Spectrum β-lactamase (ESBL) And Carbapenemase Encoding Escherichia coli isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city Tishan, Salman Habib Haque, Md Najmul Hasan, Arnab Hasanuzzaman, Md. Department of Mathematics and Natural Sciences, Brac University Escherichia coli ESBL Antimicrobial susceptibility Hospital wastewater Water-borne disease Food-borne diseases Waterborne infection Food contamination Wastewater--Microbiology Escherichia coli infections in animals Escherichia coli infections This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology and Bachelor of Science in Microbiology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 54-57). Escherichia coli is a gram-negative, rod-shaped bacterium in the family Enterobacteriaceae. E.coli is an organism of bacterium that can be identified living in a variety of conditions, such as warm blooded animals' and humans' gastrointestinal tracts, where it is a component of the gut microbiota and frequently released into the environment through wastewater effluent or diarrhea. The most common way to develop E.coli is by consuming contaminated food. In this project we collected hospital sewage water and tap water from the community which are located 300 meters from the hospital. The main purpose of our project was to identify the E.coli ESBL and carbapenems encoding strain from hospital sewage water and 300 meter range of nearby community tap water. From November 2022 to June 2023 a total 125 isolates of confirmed E.coli were found and among these 125 isolates 84 isolates were found in hospital sewage water and 41 isolates were found in community tap water which was collected from 300 meters range from the hospitals. Out of 125 isolates, 26 E.coli isolates were found to be ESBL, while the remaining 6 isolates were found to be carbapenemase encoded genes. We identified 60 out of 125 isolates from the Dhaka Shishu Hospital and nearby community. Accordingly, 52 isolates were found from the National Cancer Research Institution and Hospital. The remaining 13 isolates found out from DNCC COVID-19 Dedicated Hospital and their nearby area. After identify the 125 isolates by looking at their Antimicrobial Susceptibility Test (AST) pattern, 64 isolates were selected based on their phenotypic characteristic For identification of ESBL encoded E.coli from the 26 E.coli 14 isolates were found positive for CTX-M (53.8%) and 12 isolates found positive (46.2%) for bla-TEM. Moreover, 18.8% isolates tested positive for NDM but no positive result for SHV and KPC for identification of carbapenemase resistance genes. After observing the ratio, the vast majority of E.coli isolates found ESBL resistant and most of the isolates identified from hospital sewage. Salman Habib Tishan Md Najmul Haque Arnab Hasan B. Microbiology B. Biotechnology 2024-06-25T04:33:06Z 2024-06-25T04:33:06Z ©2023 2023-08 Thesis ID 19136050 ID 19136056 ID 19126058 http://hdl.handle.net/10361/23564 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 65 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Escherichia coli ESBL Antimicrobial susceptibility Hospital wastewater Water-borne disease Food-borne diseases Waterborne infection Food contamination Wastewater--Microbiology Escherichia coli infections in animals Escherichia coli infections |
| spellingShingle |
Escherichia coli ESBL Antimicrobial susceptibility Hospital wastewater Water-borne disease Food-borne diseases Waterborne infection Food contamination Wastewater--Microbiology Escherichia coli infections in animals Escherichia coli infections Tishan, Salman Habib Haque, Md Najmul Hasan, Arnab Detection of Extended Spectrum β-lactamase (ESBL) And Carbapenemase Encoding Escherichia coli isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology and Bachelor of Science in Microbiology, 2023. |
| author2 |
Hasanuzzaman, Md. |
| author_facet |
Hasanuzzaman, Md. Tishan, Salman Habib Haque, Md Najmul Hasan, Arnab |
| format |
Thesis |
| author |
Tishan, Salman Habib Haque, Md Najmul Hasan, Arnab |
| author_sort |
Tishan, Salman Habib |
| title |
Detection of Extended Spectrum β-lactamase (ESBL) And Carbapenemase Encoding Escherichia coli isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| title_short |
Detection of Extended Spectrum β-lactamase (ESBL) And Carbapenemase Encoding Escherichia coli isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| title_full |
Detection of Extended Spectrum β-lactamase (ESBL) And Carbapenemase Encoding Escherichia coli isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| title_fullStr |
Detection of Extended Spectrum β-lactamase (ESBL) And Carbapenemase Encoding Escherichia coli isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| title_full_unstemmed |
Detection of Extended Spectrum β-lactamase (ESBL) And Carbapenemase Encoding Escherichia coli isolates from hospital effluent wastewater and hospital adjacent community tap water in Dhaka metropolitan city |
| title_sort |
detection of extended spectrum β-lactamase (esbl) and carbapenemase encoding escherichia coli isolates from hospital effluent wastewater and hospital adjacent community tap water in dhaka metropolitan city |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23564 |
| work_keys_str_mv |
AT tishansalmanhabib detectionofextendedspectrumblactamaseesblandcarbapenemaseencodingescherichiacoliisolatesfromhospitaleffluentwastewaterandhospitaladjacentcommunitytapwaterindhakametropolitancity AT haquemdnajmul detectionofextendedspectrumblactamaseesblandcarbapenemaseencodingescherichiacoliisolatesfromhospitaleffluentwastewaterandhospitaladjacentcommunitytapwaterindhakametropolitancity AT hasanarnab detectionofextendedspectrumblactamaseesblandcarbapenemaseencodingescherichiacoliisolatesfromhospitaleffluentwastewaterandhospitaladjacentcommunitytapwaterindhakametropolitancity |
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