Microbial determination methods in pharmaceutical and food quality control
This thesis is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2023.
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Brac University
2024
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10361-235582024-06-25T21:04:48Z Microbial determination methods in pharmaceutical and food quality control Das, Arpon Kumar Haque, Fahim Kabir Monjurul Department of Mathematics and Natural Sciences, Brac University Microbial determination Pharmaceutical industry Food quality Quality control Pharmaceutical industry--Quality control Food industry and trade--Quality control This thesis is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2023. Cataloged from PDF version of thesis. Includes bibliographical references (pages 39-58). Within the realms of pharmaceutical and food production, maintaining stringent quality control measures is imperative. The determination of microbial content emerges as a pivotal component in safeguarding the integrity and safety of these products. This in-depth investigation traversed the multifaceted landscape of microbial determination methods, encompassing diverse sample types and an array of analytical techniques. The foundational section on sampling processes meticulously outlines the intricacies involved in acquiring representative samples across various matrices. It delves into the nuances of sampling methodologies tailored for liquid, solid, and environmental samples, emphasizing the pivotal role of meticulous sampling in ensuring the accuracy and reliability of subsequent microbial analyses. The exploration extended further into conventional microbial determination methodologies, elucidating the operational nuances and utility of stalwart techniques such as the bioburden test, standard plate count, most probable number and the direct epifluorescent filter technique. Emphasizing their historical significance and established application domains, this section served as a primer to contextualize the evolution of microbial determination methods. A substantial segment of this research is dedicated to rapid microbial determination methods, presenting a thorough examination of Polymerase Chain Reaction (PCR), Enzyme-Linked Immunosorbent Assay (ELISA), ATP bioluminescence assays, and flow cytometry. This detailed analysis underscores the unparalleled speed and accuracy of these contemporary techniques, revolutionizing microbial analysis across pharmaceutical and food industries. Venturing into the cutting-edge frontiers of microbial determination, this study explored the burgeoning domain of newer rapid techniques. It provided an incisive insight into miniaturized systems, biosensors, and their recent strides, notably highlighting the advancements in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). This section encapsulates the transformative potential of these novel techniques, offering glimpses into their applications poised to redefine microbial determination across industries. In summation, this paper synthesized the intricacies and advancements discussed, underlining their profound implications in fortifying the foundations of quality control protocols. A blend of conventional, rapid and cutting-edge methods forms a robust system for precise microbe identification, bolstering safety and quality in pharmaceutical and food production. Arpon Kumar Das M. Biotechnology 2024-06-25T04:09:17Z 2024-06-25T04:09:17Z ©2023 2023-12 Thesis ID: 22176013 http://hdl.handle.net/10361/23558 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 72 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Microbial determination Pharmaceutical industry Food quality Quality control Pharmaceutical industry--Quality control Food industry and trade--Quality control |
| spellingShingle |
Microbial determination Pharmaceutical industry Food quality Quality control Pharmaceutical industry--Quality control Food industry and trade--Quality control Das, Arpon Kumar Microbial determination methods in pharmaceutical and food quality control |
| description |
This thesis is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2023. |
| author2 |
Haque, Fahim Kabir Monjurul |
| author_facet |
Haque, Fahim Kabir Monjurul Das, Arpon Kumar |
| format |
Thesis |
| author |
Das, Arpon Kumar |
| author_sort |
Das, Arpon Kumar |
| title |
Microbial determination methods in pharmaceutical and food quality control |
| title_short |
Microbial determination methods in pharmaceutical and food quality control |
| title_full |
Microbial determination methods in pharmaceutical and food quality control |
| title_fullStr |
Microbial determination methods in pharmaceutical and food quality control |
| title_full_unstemmed |
Microbial determination methods in pharmaceutical and food quality control |
| title_sort |
microbial determination methods in pharmaceutical and food quality control |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23558 |
| work_keys_str_mv |
AT dasarponkumar microbialdeterminationmethodsinpharmaceuticalandfoodqualitycontrol |
| _version_ |
1814309076032552960 |