A landscape view on antimicrobial resistance pattern of Acinetobacter baumannii and Vibrio cholerae in hospital wastewater and adjacent household water
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.
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Brac University
2024
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10361-235522024-06-25T21:02:39Z A landscape view on antimicrobial resistance pattern of Acinetobacter baumannii and Vibrio cholerae in hospital wastewater and adjacent household water Morshed, Sumaiya Khan, Shadman Sakib Ahmed, Akash Department of Mathematics and Natural Sciences, Brac University Acinetobacter baumannii Vibrio cholerae Antimicrobial resistance Hospital wastewater Wastewater--Microbiology Drug resistance Pathogenicity This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 53-67). Global threats to public health are growing from carbapenem-resistant Gram-negative bacteria, particularly the Acinetobacter baumannii-calcoaceticus complex and Enterobacteriaceae- Vibrio cholerae. Numerous multidrug-resistant strains of V. cholerae and A. baumannii have been found in both clinical and environmental settings. One of the six most significant multidrug resistant pathogens found in hospitals around the world, Acinetobacter baumannii is known for its opportunistic nosocomial infection causing abilities and Cholera is caused by the bacterium called Vibrio cholerae, which places a significant burden on global public health, particularly in developing nations like Bangladesh. The current study aims to isolate and assess the prevalence of multidrug resistant A. baumannii and V. cholerae in hospital wastewater and its adjacent household water samples from three hospitals in the heart of Dhaka-City. Throughout the study which lasted from December 2022 to February 2023, 78 confirmed A. baumanni and 60 confirmed V. cholerae isolates have been identified using PCR. These isolates were further subjected to an antibiotic susceptibility test using various first line antibiotics. All the confirmed isolates for both organisms showed great resistivity to Erythromycin and Cefixime. The resistance outcome for A. baumannii and V. cholerae are as follows: Gentamicin (0%) and (1.67%), Amikacin (1.54%) and (1.67%), Imipenem (7.69%) and (6.67%), Cefixime (89.74%) and (80%), Ceftazidime (14.1%) and (16.67%), Cefepime (24.36%) and (8.33%), Amoxicillin Clavulanic acid (21.79%) and (15%), Doxycycline (6.41%) and (20%), Aztreonam (15.38%) and (3.33%), Erythromycin (73.08%) and (100%). Furthermore, MDR genes were also detected using PCR; 1 isolate found positive for blaNDM-1 and 2 for bla-CTX-M has been found out of 29 suspected A. baumannii. On the other hand, 1 isolate has been detected for blaKPC and blaNDM-1 out of 7 suspected V. cholerae. Hence, this study highlights current developments and suggests new directions for future study, including the reservoir of carbapenem-resistant bacteria. It places a special emphasis on the evolutionary and genomic features of A.baumannii and V. cholerae in three hospitals in Dhaka City. Sumaiya Morshed B. Microbiology 2024-06-25T03:12:53Z 2024-06-25T03:12:53Z ©2023 2023-06 Thesis ID 18226007 ID 19126038 http://hdl.handle.net/10361/23552 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 68 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Acinetobacter baumannii Vibrio cholerae Antimicrobial resistance Hospital wastewater Wastewater--Microbiology Drug resistance Pathogenicity |
| spellingShingle |
Acinetobacter baumannii Vibrio cholerae Antimicrobial resistance Hospital wastewater Wastewater--Microbiology Drug resistance Pathogenicity Morshed, Sumaiya Khan, Shadman Sakib A landscape view on antimicrobial resistance pattern of Acinetobacter baumannii and Vibrio cholerae in hospital wastewater and adjacent household water |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. |
| author2 |
Ahmed, Akash |
| author_facet |
Ahmed, Akash Morshed, Sumaiya Khan, Shadman Sakib |
| format |
Thesis |
| author |
Morshed, Sumaiya Khan, Shadman Sakib |
| author_sort |
Morshed, Sumaiya |
| title |
A landscape view on antimicrobial resistance pattern of Acinetobacter baumannii and Vibrio cholerae in hospital wastewater and adjacent household water |
| title_short |
A landscape view on antimicrobial resistance pattern of Acinetobacter baumannii and Vibrio cholerae in hospital wastewater and adjacent household water |
| title_full |
A landscape view on antimicrobial resistance pattern of Acinetobacter baumannii and Vibrio cholerae in hospital wastewater and adjacent household water |
| title_fullStr |
A landscape view on antimicrobial resistance pattern of Acinetobacter baumannii and Vibrio cholerae in hospital wastewater and adjacent household water |
| title_full_unstemmed |
A landscape view on antimicrobial resistance pattern of Acinetobacter baumannii and Vibrio cholerae in hospital wastewater and adjacent household water |
| title_sort |
landscape view on antimicrobial resistance pattern of acinetobacter baumannii and vibrio cholerae in hospital wastewater and adjacent household water |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23552 |
| work_keys_str_mv |
AT morshedsumaiya alandscapeviewonantimicrobialresistancepatternofacinetobacterbaumanniiandvibriocholeraeinhospitalwastewaterandadjacenthouseholdwater AT khanshadmansakib alandscapeviewonantimicrobialresistancepatternofacinetobacterbaumanniiandvibriocholeraeinhospitalwastewaterandadjacenthouseholdwater AT morshedsumaiya landscapeviewonantimicrobialresistancepatternofacinetobacterbaumanniiandvibriocholeraeinhospitalwastewaterandadjacenthouseholdwater AT khanshadmansakib landscapeviewonantimicrobialresistancepatternofacinetobacterbaumanniiandvibriocholeraeinhospitalwastewaterandadjacenthouseholdwater |
| _version_ |
1814308095770230784 |