“Pseudomonas aeruginosa characterization and analysis based on its antimicrobial resistant pattern collected from hospital wastewater and adjacent community water.”

This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.

Chi tiết về thư mục
Những tác giả chính:	Sarah, Humaira Reza Khan, Faiza, Refat Rahman
Tác giả khác:	Ahmed, Akash
Định dạng:	Dissertation
Ngôn ngữ:	English
Được phát hành:	Brac University 2024
Những chủ đề:	Pseudomona Antimicrobial resistant Wastewater Biomolecules > Analysis.
Truy cập trực tuyến:	http://hdl.handle.net/10361/23391

id	10361-23391
record_format	dspace
spelling	10361-233912024-06-12T21:04:07Z “Pseudomonas aeruginosa characterization and analysis based on its antimicrobial resistant pattern collected from hospital wastewater and adjacent community water.” Sarah, Humaira Reza Khan Faiza, Refat Rahman Ahmed, Akash Department of Mathematics and Natural Sciences, Brac University Pseudomona Antimicrobial resistant Wastewater Biomolecules--Analysis. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. Catalogued from the PDF version of thesis. Includes bibliographical references (pages 34-40). Introduction: The main purpose of this study is to identify the multidrug-resistant genes from some specific microorganisms. Among them, our selected organism was Pseudomonas aeruginosa. The highly known Carbapenem and beta-lactamase-producing Pseudomonas aeruginosa was given priority in this study to test how much resistance and sensitivity is related to this organism in the context of Bangladesh, particularly in the highly polluted and unhealthy conditions of Dhaka city. To conduct this study different water samples from hospital waste and its surrounding selected community tap has been collected. Hospital wastewater is one of the major sources of antibiotic-resistant pathogens that is becoming an alarming concern daily. Method: This study has been from January 2023 to June 2023. A total of 54 confirmed Pseudomonas aeruginosa isolates were suspected from 78 isolates collected from hospital wastewater and adjacent community tap water. To identify exact isolates of Pseudomonas aeruginosa, the phenotypic technique was used. Also, the Antibiotic Susceptibility test helped to detect which antibiotics from different generations are susceptible or resistant against the specific isolate. Since biochemical procedures are less accurate than PCR and need additional resources and time besides these methods, we did further PCR tests to identify the resistant gene of ESBL (Extended spectrum β-lactamase) and MBLS (Metallo-beta-lactamase) after getting the multidrug-resistant isolates. ATCC strains are used as positive controls. Result: There was a total of 78 samples taken from the nearby community water and the three hospitals, and 54 of those 78 samples contained Pseudomonas aeruginosa isolates that could be confirmed. There are 69.23% confirmed isolates. These 54 confirmed isolates showed resistance to different 12 antibiotics. The hospital water showed the most resistance towards amoxyclav (94%) and then towards cefixime and ceftriaxon (82%). Whereas, the community water isolates resistance was quite similar. The highest resistance they showed towards amoxiclav (90%) and the second highest is cefixime (82%). Among all these isolates 7 isolates were chosen that showed the most resistance and further identification of Multidrug Resistance were done. Among them, for Bla-CtxM and Bla-SHV, two isolates from the June sample had positive results, or 28.5% of the total and one isolate from the June sample tested positive for Bla-oxa 48, or 14.2% of the total. Conclusion: The quality of drinking water, distribution lines, and waste management of hospital water have now become key concerns for the spread of antibiotic resistance among various species, particularly for Pseudomonas aeruginosa. So, the goal of this study is to find out if this multi-drug-resistant bacteria is present in the wastewater of the hospital and its adjacent community water. Humaira Reza Khan Sarah Refat Rahman Faiza B. Microbiology 2024-06-12T05:51:57Z 2024-06-12T05:51:57Z 2023 2023-08 Dissertation ID 16204088 ID 19326027 http://hdl.handle.net/10361/23391 en Brac University dissertations are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 40 pages application/pdf Brac University
institution	Brac University
collection	Institutional Repository
language	English
topic	Pseudomona Antimicrobial resistant Wastewater Biomolecules--Analysis.
spellingShingle	Pseudomona Antimicrobial resistant Wastewater Biomolecules--Analysis. Sarah, Humaira Reza Khan Faiza, Refat Rahman “Pseudomonas aeruginosa characterization and analysis based on its antimicrobial resistant pattern collected from hospital wastewater and adjacent community water.”
description	This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.
author2	Ahmed, Akash
author_facet	Ahmed, Akash Sarah, Humaira Reza Khan Faiza, Refat Rahman
format	Dissertation
author	Sarah, Humaira Reza Khan Faiza, Refat Rahman
author_sort	Sarah, Humaira Reza Khan
title	“Pseudomonas aeruginosa characterization and analysis based on its antimicrobial resistant pattern collected from hospital wastewater and adjacent community water.”
title_short	“Pseudomonas aeruginosa characterization and analysis based on its antimicrobial resistant pattern collected from hospital wastewater and adjacent community water.”
title_full	“Pseudomonas aeruginosa characterization and analysis based on its antimicrobial resistant pattern collected from hospital wastewater and adjacent community water.”
title_fullStr	“Pseudomonas aeruginosa characterization and analysis based on its antimicrobial resistant pattern collected from hospital wastewater and adjacent community water.”
title_full_unstemmed	“Pseudomonas aeruginosa characterization and analysis based on its antimicrobial resistant pattern collected from hospital wastewater and adjacent community water.”
title_sort	“pseudomonas aeruginosa characterization and analysis based on its antimicrobial resistant pattern collected from hospital wastewater and adjacent community water.”
publisher	Brac University
publishDate	2024
url	http://hdl.handle.net/10361/23391
work_keys_str_mv	AT sarahhumairarezakhan pseudomonasaeruginosacharacterizationandanalysisbasedonitsantimicrobialresistantpatterncollectedfromhospitalwastewaterandadjacentcommunitywater AT faizarefatrahman pseudomonasaeruginosacharacterizationandanalysisbasedonitsantimicrobialresistantpatterncollectedfromhospitalwastewaterandadjacentcommunitywater
_version_	1814309075297501184

“Pseudomonas aeruginosa characterization and analysis based on its antimicrobial resistant pattern collected from hospital wastewater and adjacent community water.”

Những quyển sách tương tự