Study of viral load reduction in disposable face masks upon UV-C exposure using RT-qPCR
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2022.
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Brac University
2024
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| 在线阅读: | http://hdl.handle.net/10361/23161 |
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10361-23161 |
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10361-231612024-06-05T21:05:41Z Study of viral load reduction in disposable face masks upon UV-C exposure using RT-qPCR Sharfuddin, A.A.M Jamiruddin, Mohd. Raeed School of Pharmacy, Brac University UV-C exposure RNA extraction UV-C chamber RT-qPCR Polymerase chain reaction Molecular diagnosis This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2022. Cataloged from the PDF version of thesis. Includes bibliographical references (pages 33-42). This research is designed to promote the long-term usage of surgical masks, proper disposal, environmental protection, controlled production and the developed UV-C chambers validation. Moreover, we sought to evaluate the effectiveness of our developed decontamination technique for surgical masks using UV-C irradiation, as there is no established guideline for the safe reuse of surgical masks. Masks were collected from the patients and included in the experiment if the presence of the Covid-19 virus was ensured. These used masks were treated with UV-C to decontaminate them and RT-qPCR analysis was used to determine whether the Covid-19 virus was present. The final evaluation was done by comparing the RT-qPCR results of before and after UV-C exposed samples. The established results are quite preferable and consistent with the hypothesis and the UV-C exposure rendered the Covid-19-causing genes inactive. Using the UV-C chamber to reuse the masks could be a new addition of technology in recent eras, which may ensure the rational use of masks. A.A.M Sharfuddin B. Pharmacy 2024-06-05T09:17:26Z 2024-06-05T09:17:26Z ©2022 2022-10 Thesis ID: 18346053 http://hdl.handle.net/10361/23161 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 43 pages application/pdf Brac University |
| institution |
Brac University |
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Institutional Repository |
| language |
English |
| topic |
UV-C exposure RNA extraction UV-C chamber RT-qPCR Polymerase chain reaction Molecular diagnosis |
| spellingShingle |
UV-C exposure RNA extraction UV-C chamber RT-qPCR Polymerase chain reaction Molecular diagnosis Sharfuddin, A.A.M Study of viral load reduction in disposable face masks upon UV-C exposure using RT-qPCR |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2022. |
| author2 |
Jamiruddin, Mohd. Raeed |
| author_facet |
Jamiruddin, Mohd. Raeed Sharfuddin, A.A.M |
| format |
Thesis |
| author |
Sharfuddin, A.A.M |
| author_sort |
Sharfuddin, A.A.M |
| title |
Study of viral load reduction in disposable face masks upon UV-C exposure using RT-qPCR |
| title_short |
Study of viral load reduction in disposable face masks upon UV-C exposure using RT-qPCR |
| title_full |
Study of viral load reduction in disposable face masks upon UV-C exposure using RT-qPCR |
| title_fullStr |
Study of viral load reduction in disposable face masks upon UV-C exposure using RT-qPCR |
| title_full_unstemmed |
Study of viral load reduction in disposable face masks upon UV-C exposure using RT-qPCR |
| title_sort |
study of viral load reduction in disposable face masks upon uv-c exposure using rt-qpcr |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23161 |
| work_keys_str_mv |
AT sharfuddinaam studyofviralloadreductionindisposablefacemasksuponuvcexposureusingrtqpcr |
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1814309594228326400 |