Characterization of newly identified bacterial biofilm degrading bacteriophage obtained from Dhaka city lake water samples
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023.
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| Langue: | English |
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Brac University
2024
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| Accès en ligne: | http://hdl.handle.net/10361/23134 |
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10361-231342024-06-05T21:01:15Z Characterization of newly identified bacterial biofilm degrading bacteriophage obtained from Dhaka city lake water samples Mahdee, Munif Abdullah, Syed Ridwan Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University Biofilm Lake water Dhaka city Biotechnology. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. Catalogued from the PDF version of thesis. Includes bibliographical references (pages 43-44). Recently acquired bacteriophages from water samples in Dhaka city which degraded the bacterial biofilms were characterized in this investigation. Bacteriophages unique to E. coli strain 9A(ⲫ9A) and Vibrio cholerae strain 13B(ⲫ13B) were identified. Throughout the process, strict laboratory protocols were followed, and the characterization was based on three variables: temperature, pH, and salinity. In the first step of the procedure, single colonies of host bacteria were obtained by streak plating, which was followed by enrichment of the corresponding bacteriophages. The Phage counts were then determined using the Double Layer Assay (DLA). To begin DLA, single colonies in LB broth were first taken and cultured in a shaker. The bacteriophages underwent a series of dilutions before the phages and the bacteria were added in the molten soft agar. After being centrifuged, the soft agar was quickly placed onto a Luria Bertani Agar plate. After evenly distributing the materials, the plate was swirled and until the agar solidified and was then placed in an incubator for 16 hours. The following day, the plaques were counted, and the number of Plaque Forming Units per Milliliter (PFU/ml) were determined. An average phage count was discovered after the experiment was conducted three times for each component. Both ⲫ9A and ⲫ13B displayed unusual results in temperature and pH readings in addition to salinity. Munif Mahdee Syed Ridwan Abdullah B. Biotechnology 2024-06-05T04:17:41Z 2024-06-05T04:17:41Z 2023 2023-09 Thesis ID 19236007 ID 19236008 http://hdl.handle.net/10361/23134 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 44 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Biofilm Lake water Dhaka city Biotechnology. |
| spellingShingle |
Biofilm Lake water Dhaka city Biotechnology. Mahdee, Munif Abdullah, Syed Ridwan Characterization of newly identified bacterial biofilm degrading bacteriophage obtained from Dhaka city lake water samples |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Mahdee, Munif Abdullah, Syed Ridwan |
| format |
Thesis |
| author |
Mahdee, Munif Abdullah, Syed Ridwan |
| author_sort |
Mahdee, Munif |
| title |
Characterization of newly identified bacterial biofilm degrading bacteriophage obtained from Dhaka city lake water samples |
| title_short |
Characterization of newly identified bacterial biofilm degrading bacteriophage obtained from Dhaka city lake water samples |
| title_full |
Characterization of newly identified bacterial biofilm degrading bacteriophage obtained from Dhaka city lake water samples |
| title_fullStr |
Characterization of newly identified bacterial biofilm degrading bacteriophage obtained from Dhaka city lake water samples |
| title_full_unstemmed |
Characterization of newly identified bacterial biofilm degrading bacteriophage obtained from Dhaka city lake water samples |
| title_sort |
characterization of newly identified bacterial biofilm degrading bacteriophage obtained from dhaka city lake water samples |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23134 |
| work_keys_str_mv |
AT mahdeemunif characterizationofnewlyidentifiedbacterialbiofilmdegradingbacteriophageobtainedfromdhakacitylakewatersamples AT abdullahsyedridwan characterizationofnewlyidentifiedbacterialbiofilmdegradingbacteriophageobtainedfromdhakacitylakewatersamples |
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1814307373874937856 |