Bacteriological study of Payra river: microbial isolation, identification, analysis of antimicrobial resistance and pathogenicity
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022.
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Brac University
2024
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| Accès en ligne: | http://hdl.handle.net/10361/23079 |
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10361-230792024-06-03T21:04:44Z Bacteriological study of Payra river: microbial isolation, identification, analysis of antimicrobial resistance and pathogenicity Rahman, Ismam Nashid Shudha, Farzana Yeasmin Ebnat, Kazi Anika Fatima, Nayara Noor-E Ahmed , Akash Department of Mathematics and Natural Sciences, Brac University River water Antimicrobial Resistance (AMR) Pathogenicity Staphylococcus spp. E. coli Bacillus spp. Antimicrobial resistance This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022. Catalogued from PDF version of thesis. Includes bibliographical references (pages 30-33). "Introduction: Water is the most precious element for all living creatures, being used for drinking, bathing, domestic purposes, etc. Being a riverine country, Bangladesh's people depend heavily on river water. This causes the water to be contaminated in various ways that raise serious public health concerns in terms of infection from antibiotic-resistant bacteria. Methodology: 21 samples were collected from 7 sampling sites in Patuakhali and processed within 24 hours of collection. The samples were spread on several selective media: following which 47 isolates were selected based on distinct morphologies. Several biochemical tests were employed for initial identification, after which antibiotic susceptibility against Amikacin, Amoxicillin, Amoxyclav, Azithromycin, Cefixime, Ceftriaxone, Colistin, Erythromycin, Levofloxacin, Meropenem, Penicillin, Tetracycline, and Vancomycin was examined. DNAse and coagulase tests were investigated to determine the extent of pathogenicity. Results: In this study, out of 47 isolates, Staphylococcus spp was the most predominant (20%) in the water samples, followed by Escherichia coli (16%), and Bacillus spp. (14%). Among the isolates, 51% and 68% were proven to be coagulase and DNase positive respectively, confirming the pathogenicity of our organisms. 94% of the isolates were penicillin-resistant followed by 73% amoxiclav-resistant. However, 100% and 95% of the isolates were susceptible to meropenem and levofloxacin respectively. Conclusion: The results from our study form the baseline data for the bacteriology of Payra river which emphasizes the presence of enteric coliforms, especially Escherichia coli followed by Serratia spp., Streptococcus spp, Salmonella spp., Klebsiella spp., Enterococcus spp.,. and Pseudomonas aeuruginosa. Pathogenic strains were detected which increase the risk of infections. In addition, the high rate of DNAse and Coagulase positive isolates shows the extent of contamination which can spread several diseases among the dwellers who use water from this river." Ismam Nashid Rahman Farzana Yeasmin Shudha Kazi Anika Ebnat Nayara Noor-E Fatima B. Microbiology 2024-06-03T05:29:53Z 2024-06-03T05:29:53Z 2022 2022-12 Thesis ID 18126080 ID 17226015 ID 18126026 ID 18226005 http://hdl.handle.net/10361/23079 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 41 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
River water Antimicrobial Resistance (AMR) Pathogenicity Staphylococcus spp. E. coli Bacillus spp. Antimicrobial resistance |
| spellingShingle |
River water Antimicrobial Resistance (AMR) Pathogenicity Staphylococcus spp. E. coli Bacillus spp. Antimicrobial resistance Rahman, Ismam Nashid Shudha, Farzana Yeasmin Ebnat, Kazi Anika Fatima, Nayara Noor-E Bacteriological study of Payra river: microbial isolation, identification, analysis of antimicrobial resistance and pathogenicity |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022. |
| author2 |
Ahmed , Akash |
| author_facet |
Ahmed , Akash Rahman, Ismam Nashid Shudha, Farzana Yeasmin Ebnat, Kazi Anika Fatima, Nayara Noor-E |
| format |
Thesis |
| author |
Rahman, Ismam Nashid Shudha, Farzana Yeasmin Ebnat, Kazi Anika Fatima, Nayara Noor-E |
| author_sort |
Rahman, Ismam Nashid |
| title |
Bacteriological study of Payra river: microbial isolation, identification, analysis of antimicrobial resistance and pathogenicity |
| title_short |
Bacteriological study of Payra river: microbial isolation, identification, analysis of antimicrobial resistance and pathogenicity |
| title_full |
Bacteriological study of Payra river: microbial isolation, identification, analysis of antimicrobial resistance and pathogenicity |
| title_fullStr |
Bacteriological study of Payra river: microbial isolation, identification, analysis of antimicrobial resistance and pathogenicity |
| title_full_unstemmed |
Bacteriological study of Payra river: microbial isolation, identification, analysis of antimicrobial resistance and pathogenicity |
| title_sort |
bacteriological study of payra river: microbial isolation, identification, analysis of antimicrobial resistance and pathogenicity |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23079 |
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