Investigating adaptation of Staphylococcus aureus to hand sanitisers and subsequent antibiotic co-selection via experimental evolution
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.
| Những tác giả chính: | , |
|---|---|
| Tác giả khác: | |
| Định dạng: | Luận văn |
| Ngôn ngữ: | English |
| Được phát hành: |
Brac University
2024
|
| Những chủ đề: | |
| Truy cập trực tuyến: | http://hdl.handle.net/10361/23074 |
| id |
10361-23074 |
|---|---|
| record_format |
dspace |
| spelling |
10361-230742024-06-03T21:01:20Z Investigating adaptation of Staphylococcus aureus to hand sanitisers and subsequent antibiotic co-selection via experimental evolution Seam Alam, Shamrin Adiba Siddiqee, Mahbubul Hasan Department of Mathematics and Natural Sciences, Brac University Hand sanitiser Bacterial adaptation Antibiotic co-selection Chlorhexidine Bacteria--Adhesion Chlorhexidine This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 70-85). One of the most notable results of the COVID-19 pandemic is the escalation in the use of hand sanitisers as personal hygiene products as well as in healthcare settings. Besides devising effective infection control strategies, understanding how bacterial strains adapt to these agents is crucial, especially due to the possibility of an unwanted evolution of antibiotic-resistant strains. This study explored how Staphylococcus aureus responds to some common hand sanitiser formulations. We investigated microbial tolerance acquisition and potential antibiotic co-selection through experimental evolution. S. aureus was exposed to escalating subinhibitory hand sanitiser concentrations of Hexisol® (0.5% chlorhexidine gluconate & 70% isopropyl alcohol), Sepnil® (70% ethanol, carbomer, glycerin, polyethylene glycol, TEA, aqua, and perfume), and 70% Ethanol. We compared the Minimum Inhibitory Concentrations (MIC) between evolved and parent strains, performed antibiotic susceptibility testing, assessed fitness using a growth curve assay, and subcultured the evolved strains in sanitiser-free broth for 10 days to assess the stability of the acquired tolerance. Hexisol® showed a 4-fold increase in MIC against the adapted strain, co-selection for macrolide and β-lactam antibiotics, and increased susceptibility to other antibiotics. The growth capacity of the adapted strain was significantly decreased. Tolerance to Hexisol® remained stable, but antibiotic co-selection reversed after 10 days. 70% ethanol and Sepnil® showed similar results. The MIC of these hand sanitisers against the adapted strain did not significantly increase; however, it exceeded the recommended dose. No antibiotic co-selection occurred, but susceptibility to certain antibiotics increased. The growth capacity of the adapted strain was significantly decreased. Bacterial tolerance to 70% ethanol and Sepnil® remained stable after 10 subcultures. The findings shed light on the emergence of potential public health hazards resulting from microbial adaptation to hand sanitisers, emphasizing the need for reevaluation of their long-term impact on antibiotic resistance and infection control strategies. Seam Shamrin Adiba Alam B. Microbiology 2024-06-03T04:41:59Z 2024-06-03T04:41:59Z ©2023 2023-10 Thesis ID 19236020 ID 19136006 http://hdl.handle.net/10361/23074 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 101 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Hand sanitiser Bacterial adaptation Antibiotic co-selection Chlorhexidine Bacteria--Adhesion Chlorhexidine |
| spellingShingle |
Hand sanitiser Bacterial adaptation Antibiotic co-selection Chlorhexidine Bacteria--Adhesion Chlorhexidine Seam Alam, Shamrin Adiba Investigating adaptation of Staphylococcus aureus to hand sanitisers and subsequent antibiotic co-selection via experimental evolution |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. |
| author2 |
Siddiqee, Mahbubul Hasan |
| author_facet |
Siddiqee, Mahbubul Hasan Seam Alam, Shamrin Adiba |
| format |
Thesis |
| author |
Seam Alam, Shamrin Adiba |
| author_sort |
Seam |
| title |
Investigating adaptation of Staphylococcus aureus to hand sanitisers and subsequent antibiotic co-selection via experimental evolution |
| title_short |
Investigating adaptation of Staphylococcus aureus to hand sanitisers and subsequent antibiotic co-selection via experimental evolution |
| title_full |
Investigating adaptation of Staphylococcus aureus to hand sanitisers and subsequent antibiotic co-selection via experimental evolution |
| title_fullStr |
Investigating adaptation of Staphylococcus aureus to hand sanitisers and subsequent antibiotic co-selection via experimental evolution |
| title_full_unstemmed |
Investigating adaptation of Staphylococcus aureus to hand sanitisers and subsequent antibiotic co-selection via experimental evolution |
| title_sort |
investigating adaptation of staphylococcus aureus to hand sanitisers and subsequent antibiotic co-selection via experimental evolution |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23074 |
| work_keys_str_mv |
AT seam investigatingadaptationofstaphylococcusaureustohandsanitisersandsubsequentantibioticcoselectionviaexperimentalevolution AT alamshamrinadiba investigatingadaptationofstaphylococcusaureustohandsanitisersandsubsequentantibioticcoselectionviaexperimentalevolution |
| _version_ |
1814307373548830720 |