Unveiling the role of free DNA in biofilm formation; potential relevance in Cholera epidemiology
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023.
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Brac University
2024
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10361-230702024-09-17T21:01:19Z Unveiling the role of free DNA in biofilm formation; potential relevance in Cholera epidemiology Afrid, Abir Hassan Rahman, Mohammad Rafiue Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University Biofilm formation Genome DNA Isogenic Heterogenic Biofilms--growth & development. Gene mapping. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 93-106). This study meticulously probes the impact of both isogenic and heterogenic genomic DNA, in its entirety and fragmented states, on the density of biofilms formed by distinct bacterial strains, spanning both gram-positive and gram-negative classifications. Utilising samples from two distinct strains of Vibrio cholerae (WT346 and 1877), Escherichia coli (0157:H7), and Staphylococcus aureus (ATCC 25923), which were procured from the Life and Natural Sciences Laboratory at BRAC University, the formation and robustness of biofilms were quantitatively analysed via the crystal violet assay technique. Notably, biofilm density exhibited pronounced variations when exposed to isogenic genomic DNA as opposed to its heterogenic counterpart. Moreover, intact genomic DNA consistently elicited enhanced biofilm thickness relative to its fragmented variant, which elicited a more heterogeneous and attenuated response. Among the bacterial exemplars scrutinised, strain 1877 exhibited the most pronounced percentage augmentation in biofilm density in the presence of both fragmented (150%) and whole (125%) ATCC 25923 genomic DNA. This investigation underscores the nuanced interplay between genomic DNA and bacterial biofilm dynamics, offering pivotal insights for microbial ecology. Abir Hassan Afrid Mohammad Rafiue Rahman B. Biotechnology 2024-06-03T04:07:13Z 2024-06-03T04:07:13Z 2023-10 Thesis ID 19136005 ID 19136049 http://hdl.handle.net/10361/23070 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 118 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Biofilm formation Genome DNA Isogenic Heterogenic Biofilms--growth & development. Gene mapping. |
| spellingShingle |
Biofilm formation Genome DNA Isogenic Heterogenic Biofilms--growth & development. Gene mapping. Afrid, Abir Hassan Rahman, Mohammad Rafiue Unveiling the role of free DNA in biofilm formation; potential relevance in Cholera epidemiology |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Afrid, Abir Hassan Rahman, Mohammad Rafiue |
| format |
Thesis |
| author |
Afrid, Abir Hassan Rahman, Mohammad Rafiue |
| author_sort |
Afrid, Abir Hassan |
| title |
Unveiling the role of free DNA in biofilm formation; potential relevance in Cholera epidemiology |
| title_short |
Unveiling the role of free DNA in biofilm formation; potential relevance in Cholera epidemiology |
| title_full |
Unveiling the role of free DNA in biofilm formation; potential relevance in Cholera epidemiology |
| title_fullStr |
Unveiling the role of free DNA in biofilm formation; potential relevance in Cholera epidemiology |
| title_full_unstemmed |
Unveiling the role of free DNA in biofilm formation; potential relevance in Cholera epidemiology |
| title_sort |
unveiling the role of free dna in biofilm formation; potential relevance in cholera epidemiology |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23070 |
| work_keys_str_mv |
AT afridabirhassan unveilingtheroleoffreednainbiofilmformationpotentialrelevanceincholeraepidemiology AT rahmanmohammadrafiue unveilingtheroleoffreednainbiofilmformationpotentialrelevanceincholeraepidemiology |
| _version_ |
1814307373386301440 |