PCR based detection of helicobacter pylori compared with CLO in stomach biopsy samples from patients with dyspepsia: a pilot study
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023.
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| التنسيق: | أطروحة |
| اللغة: | English |
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Brac University
2024
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://hdl.handle.net/10361/23059 |
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10361-23059 |
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10361-230592024-06-02T21:04:32Z PCR based detection of helicobacter pylori compared with CLO in stomach biopsy samples from patients with dyspepsia: a pilot study Zaker, Bushra Binte Akhond, Saraf Nahar Nanchi, Shomia Haque, Fahim Kabir Monjural Department of Mathematics and Natural Sciences, Brac University Polymerase chain reaction Biopsy DNA extraction Dyspepsia Polymerase chain reaction Biopsy--methods DNA fingerprinting Dyspepsia This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 28-31). Helicobacter pylori infection can lead to a range of gastrointestinal diseases, including gastritis and peptic ulcers, and can develop into stomach cancer if left untreated. Treating H. pylori infection in an early stage is crucial to mitigate these health risks and improve digestive health. The CLO test is the primary method for clinical H. pylori detection due to its ease of use and time efficiency. We collected seven biopsy samples through endoscopy from patients exhibiting symptoms of dyspepsia. The samples were subjected to molecular diagnostic procedures in the laboratory of Brac University. DNA extraction was carried out followed by polymerase chain reaction (PCR) using specific primers of two genes, UreA and 23S rRNA. It was conducted in order to isolate and amplify the DNA of H. pylori. In CLO testing, 71% of the selected patients tested positive for H. pylori infection, whereas only 29% of the patient pool was found to be positive for both the UreA gene and the 23S rRNA gene. Such discrepancies in positivity rates raise questions about the effectiveness of the standard testing method (CLO) for detecting an intensive infection like H. pylori. Bushra Binte Zaker Saraf Akhond Shomia Nahar Nanchi B. Biotechnology 2024-06-02T09:23:59Z 2024-06-02T09:23:59Z ©2023 2023-10 Thesis ID 18336032 ID 18336021 ID 18336017 http://hdl.handle.net/10361/23059 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 43 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Polymerase chain reaction Biopsy DNA extraction Dyspepsia Polymerase chain reaction Biopsy--methods DNA fingerprinting Dyspepsia |
| spellingShingle |
Polymerase chain reaction Biopsy DNA extraction Dyspepsia Polymerase chain reaction Biopsy--methods DNA fingerprinting Dyspepsia Zaker, Bushra Binte Akhond, Saraf Nahar Nanchi, Shomia PCR based detection of helicobacter pylori compared with CLO in stomach biopsy samples from patients with dyspepsia: a pilot study |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. |
| author2 |
Haque, Fahim Kabir Monjural |
| author_facet |
Haque, Fahim Kabir Monjural Zaker, Bushra Binte Akhond, Saraf Nahar Nanchi, Shomia |
| format |
Thesis |
| author |
Zaker, Bushra Binte Akhond, Saraf Nahar Nanchi, Shomia |
| author_sort |
Zaker, Bushra Binte |
| title |
PCR based detection of helicobacter pylori compared with CLO in stomach biopsy samples from patients with dyspepsia: a pilot study |
| title_short |
PCR based detection of helicobacter pylori compared with CLO in stomach biopsy samples from patients with dyspepsia: a pilot study |
| title_full |
PCR based detection of helicobacter pylori compared with CLO in stomach biopsy samples from patients with dyspepsia: a pilot study |
| title_fullStr |
PCR based detection of helicobacter pylori compared with CLO in stomach biopsy samples from patients with dyspepsia: a pilot study |
| title_full_unstemmed |
PCR based detection of helicobacter pylori compared with CLO in stomach biopsy samples from patients with dyspepsia: a pilot study |
| title_sort |
pcr based detection of helicobacter pylori compared with clo in stomach biopsy samples from patients with dyspepsia: a pilot study |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23059 |
| work_keys_str_mv |
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| _version_ |
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