Comparative study on antibiotic resistance profiles and plasmid presence in water samples focusing inside and outside Dhaka district
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023.
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| Idioma: | English |
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Brac University
2024
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| Acesso em linha: | http://hdl.handle.net/10361/23042 |
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10361-230422024-06-02T21:02:21Z Comparative study on antibiotic resistance profiles and plasmid presence in water samples focusing inside and outside Dhaka district Maliha, Sumaiya Tasnim Zarin, Nusrat Kamrul Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University Water pollution Antimicrobial resistance (AMR) Gram-positive bacteria Gram-negative bacteria Drug resistance in microorganisms Water pollution Gram-negative bacterial infections This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 103-108). One of the most pressing issues of recent times is antimicrobial resistance or AMR. Nowadays, treating infections with susceptible antibiotics has become a major concern as antibiotic resistance is rapidly rising. And due to a lack of susceptible antibiotics for a specific infection, millions of deaths are occurring worldwide. This antibiotic resistance is increasing mainly as a result of the excessive use of antibiotics and improper disposal in the environment. For assessing the present state of antibiotic resistance in Bangladesh, water samples were collected from different areas of Bangladesh and a comparative analysis was done between inside and outside Dhaka district. We have collected four water samples from inside Dhaka district and four water samples from outside Dhaka district. A total of eight water samples were collected, and from those, 160 colonies were isolated. For the isolation of different bacterial species, we have used selective media, including MacConkey, SS, TCBS, and EMB. The general identification of the isolates was done using Gram staining and HiMedia morphology identification techniques. We have found different species of bacteria, including Escherichia coli, Vibrio, Shigella, Salmonella, Klebsiella, Pseudomonas, and Enterobacter. The primary goal of this research project was to conduct a comparative analysis of the antibiotic resistance profiles between inside and outside Dhaka district water sources. Here, antibiotic susceptibility testing (AST) was performed using 14 different antibiotic groups. Many multidrug-resistant bacteria were found in both inside and outside Dhaka district samples. However, the antibiotic resistance rate was found to be higher in inside Dhaka district water samples. Moreover, we have obtained plasmids from the bacterial isolates and visualized those plasmids via the gel electrophoresis method. As we know, bacteria can pick up resistant genes containing plasmids and vi become resistant to antibiotics. And after analyzing our results, similarly, we have found a correlation between the presence of plasmids in bacteria and their antibiotic resistance rate. Sumaiya Tasnim Maliha Nusrat Kamrul Zarin B. Biotechnology 2024-06-02T05:10:32Z 2024-06-02T05:10:32Z ©2023 2023-10 Thesis ID 18136048 ID 18336013 http://hdl.handle.net/10361/23042 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 121 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Water pollution Antimicrobial resistance (AMR) Gram-positive bacteria Gram-negative bacteria Drug resistance in microorganisms Water pollution Gram-negative bacterial infections |
| spellingShingle |
Water pollution Antimicrobial resistance (AMR) Gram-positive bacteria Gram-negative bacteria Drug resistance in microorganisms Water pollution Gram-negative bacterial infections Maliha, Sumaiya Tasnim Zarin, Nusrat Kamrul Comparative study on antibiotic resistance profiles and plasmid presence in water samples focusing inside and outside Dhaka district |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Maliha, Sumaiya Tasnim Zarin, Nusrat Kamrul |
| format |
Thesis |
| author |
Maliha, Sumaiya Tasnim Zarin, Nusrat Kamrul |
| author_sort |
Maliha, Sumaiya Tasnim |
| title |
Comparative study on antibiotic resistance profiles and plasmid presence in water samples focusing inside and outside Dhaka district |
| title_short |
Comparative study on antibiotic resistance profiles and plasmid presence in water samples focusing inside and outside Dhaka district |
| title_full |
Comparative study on antibiotic resistance profiles and plasmid presence in water samples focusing inside and outside Dhaka district |
| title_fullStr |
Comparative study on antibiotic resistance profiles and plasmid presence in water samples focusing inside and outside Dhaka district |
| title_full_unstemmed |
Comparative study on antibiotic resistance profiles and plasmid presence in water samples focusing inside and outside Dhaka district |
| title_sort |
comparative study on antibiotic resistance profiles and plasmid presence in water samples focusing inside and outside dhaka district |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23042 |
| work_keys_str_mv |
AT malihasumaiyatasnim comparativestudyonantibioticresistanceprofilesandplasmidpresenceinwatersamplesfocusinginsideandoutsidedhakadistrict AT zarinnusratkamrul comparativestudyonantibioticresistanceprofilesandplasmidpresenceinwatersamplesfocusinginsideandoutsidedhakadistrict |
| _version_ |
1814308270299414528 |