Effects of vitamin C in enhancing the activities of antibiotics against multi-drug resistant bacteria
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023.
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Brac University
2024
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10361-230402024-06-02T21:02:00Z Effects of vitamin C in enhancing the activities of antibiotics against multi-drug resistant bacteria Yousuf, Marzia Progya, Afia Naz Ahmed, Akash Department of Mathematics and Natural Sciences, Brac University Antibiotic resistance Antibiotic susceptibility testing Vitamin C Antimicrobial Resistance Vitamin C This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 63-66). The emergence and proliferation of antibiotic-resistant pathogens have become a global threat. A large number of the pathogens are becoming resistant even to the last resort of antibiotics. The project aimed to study the antimicrobial effect of adjuvants against multi-drug-resistant bacteria by using Vitamin C in three different concentrations. The concentrations are 1.66 mg/ml, 3.33 mg/ml, and 6.66mg/ml. Six isolates of Klebsiella pneumonia were taken. Among them five isolates (KPJANCH1, KPFEBNCH6, KPMARDSH4, H1NONCH, KPFEBDSH2) became susceptible to Amikacin in all three concentrations, among them one isolate H1NONCH became susceptible to Imipenem in all three concentrations, one isolate KPJANCH1 to Amoxicillin + Clavulanic acid (1.66mg/ml, 6.66mg/ml) but KPFEBDSH2 showed susceptibility to Cefixime (1.66 mg/ml and 3.33 mg/ml), two isolates (KPFEBNCH6, KPFEBDSH2) showed susceptibility to Azithromycin, KPFEBDSH2 in all the concentration but KPFEBNCH6 remained resistant in 3.33 mg/ml. KPFEBDSH2 also became susceptible to Ciprofloxacin and Cefixime (1.66mg/ml, 3.33mg/ml). KPFEBNCH6, KPMARDSH4, and KBFEBDSH2 became sensitive to Co-trimoxazole only in all the concentrations and KPMARDSH4 to Ceftriaxone (1.66 mg/ml). Four multi-drug-resistant E. coli isolates were treated with Vitamin C along with antibiotics, one of them remained resistant in all the concentrations by ten antibiotics and the rest of them showed susceptibility in the mentioned concentrations of Vitamin C, two isolates, E213 (6.66 mg/ml) and E238 (3.33 mg/ml) showed susceptibility to Imipenem, E238 also became susceptible to Amoxicillin + Clavulanic acid (6.66 mg/ml). Another isolate 254 by Azithromycin (3.33 mg/ml). From 6 Multi-drug resistant isolates of Staphylococcus aureus, 4 isolates showed susceptibility. Among them, three isolates (JUDNCH, SA-OTDSH-12, SEPNCW1-1) became susceptible to Amikacin in the first concentration but JUDNCH and SA-OTDSH-12 showed susceptibility at a Vitamin C concentration of 3.33 mg/ml and 6.66 mg/ml as well. For Gentamycin, SA-OTDSH-12 became susceptible in all the concentrations of Vitamin C, and SEPNCW1-1 showed sensitivity at 1.66 mg/ml. Two isolates (SEPDNCW3-15, SEPNCW1-1) became susceptible to Ciprofloxacin in all three concentrations of vitamin C. Lastly, one isolate (NONCW2-15) became susceptible to Amoxicillin + Clavulanic acid in the last two concentration. SA-SEPCNW-2 showed susceptibility to Ceftriaxone when combined with Vitamin C at a concentration of 6.66mg/ml. However, the result of remnant isolates of Klebsiella pneumonia, E. coli, 6 and Staphylococcus aureus remained indifferent. Further research is needed to understand the underlying effect of vitamin C as an adjuvant. Marzia Yousuf Afia Naz Progya B. Biotechnology 2024-06-02T04:47:10Z 2024-06-02T04:47:10Z ©2023 2023-09 Thesis ID 18236013 ID 18236001 http://hdl.handle.net/10361/23040 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 69 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Antibiotic resistance Antibiotic susceptibility testing Vitamin C Antimicrobial Resistance Vitamin C |
| spellingShingle |
Antibiotic resistance Antibiotic susceptibility testing Vitamin C Antimicrobial Resistance Vitamin C Yousuf, Marzia Progya, Afia Naz Effects of vitamin C in enhancing the activities of antibiotics against multi-drug resistant bacteria |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. |
| author2 |
Ahmed, Akash |
| author_facet |
Ahmed, Akash Yousuf, Marzia Progya, Afia Naz |
| format |
Thesis |
| author |
Yousuf, Marzia Progya, Afia Naz |
| author_sort |
Yousuf, Marzia |
| title |
Effects of vitamin C in enhancing the activities of antibiotics against multi-drug resistant bacteria |
| title_short |
Effects of vitamin C in enhancing the activities of antibiotics against multi-drug resistant bacteria |
| title_full |
Effects of vitamin C in enhancing the activities of antibiotics against multi-drug resistant bacteria |
| title_fullStr |
Effects of vitamin C in enhancing the activities of antibiotics against multi-drug resistant bacteria |
| title_full_unstemmed |
Effects of vitamin C in enhancing the activities of antibiotics against multi-drug resistant bacteria |
| title_sort |
effects of vitamin c in enhancing the activities of antibiotics against multi-drug resistant bacteria |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23040 |
| work_keys_str_mv |
AT yousufmarzia effectsofvitamincinenhancingtheactivitiesofantibioticsagainstmultidrugresistantbacteria AT progyaafianaz effectsofvitamincinenhancingtheactivitiesofantibioticsagainstmultidrugresistantbacteria |
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