Correlation between the prevalence of plasmids and AMR genes in the environmental isolates of different bacterial species
This thesis is submitted in partial fulfillment of the requirements for the degree of Masters of Science in Biotechnology, 2023.
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2024
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10361-230392024-06-02T21:03:17Z Correlation between the prevalence of plasmids and AMR genes in the environmental isolates of different bacterial species Rahman, Wasifa Ar Naser, Iftekhar Bin Department of Mathematics and Natural Sciences, Brac University Bacterial gene transfer Plasmids AMR genes Horizontal gene transfer (HGT) Bacterial genetic engineering Plasmids Bacterial spores This thesis is submitted in partial fulfillment of the requirements for the degree of Masters of Science in Biotechnology, 2023. Catalogued from PDF version of thesis. Includes bibliographical references (pages 22-24). Bacterial gene transfer is responsible for spread of antibiotic resistance in bacteria and evolution of bacteria which can be a threat of virulence as well play role in maintenance. Horizontal gene transfer (HGT) is the key process for transferring gene. It involves three mechanisms- conjugation, transformation and transduction. To distantly related species of bacteria, via HGT, exogenous DNA can be transferred from one bacterium to another. Apart from HGT, prophage induction and infection can be a way of transferring gene. For this, the prophage is induced by UV radiation and infects with bacterial competent cell to make the new viral particles. On the infection with other bacteria, the prophage can carry the gene through plasmid packaging or miss packaging and may also be integrated to the chromosomal DNA of the newly formed particle. Following that, in preliminary selection it showed growth of resistant colonies of its bacteria insuring of gene transfer. Further for genetic validation, plasmid isolation assures of plasmid packaging, here the result was negative for plasmid packaging thus it indicates of gene transferring and integrating to the chromosomal DNA. To validate the result, more advanced study and farther genetic validation is required like polymerase chain reaction to assure the presence of the antibiotic resistant gene in the infected particles. Wasifa Ar Rahman B. Biotechnology 2024-06-02T04:18:00Z 2024-06-02T04:18:00Z ©2023 2023-07 Thesis ID 21276003 http://hdl.handle.net/10361/23039 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 39 pages application/pdf |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Bacterial gene transfer Plasmids AMR genes Horizontal gene transfer (HGT) Bacterial genetic engineering Plasmids Bacterial spores |
| spellingShingle |
Bacterial gene transfer Plasmids AMR genes Horizontal gene transfer (HGT) Bacterial genetic engineering Plasmids Bacterial spores Rahman, Wasifa Ar Correlation between the prevalence of plasmids and AMR genes in the environmental isolates of different bacterial species |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Masters of Science in Biotechnology, 2023. |
| author2 |
Naser, Iftekhar Bin |
| author_facet |
Naser, Iftekhar Bin Rahman, Wasifa Ar |
| format |
Thesis |
| author |
Rahman, Wasifa Ar |
| author_sort |
Rahman, Wasifa Ar |
| title |
Correlation between the prevalence of plasmids and AMR genes in the environmental isolates of different bacterial species |
| title_short |
Correlation between the prevalence of plasmids and AMR genes in the environmental isolates of different bacterial species |
| title_full |
Correlation between the prevalence of plasmids and AMR genes in the environmental isolates of different bacterial species |
| title_fullStr |
Correlation between the prevalence of plasmids and AMR genes in the environmental isolates of different bacterial species |
| title_full_unstemmed |
Correlation between the prevalence of plasmids and AMR genes in the environmental isolates of different bacterial species |
| title_sort |
correlation between the prevalence of plasmids and amr genes in the environmental isolates of different bacterial species |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/23039 |
| work_keys_str_mv |
AT rahmanwasifaar correlationbetweentheprevalenceofplasmidsandamrgenesintheenvironmentalisolatesofdifferentbacterialspecies |
| _version_ |
1814308774474678272 |