In-silico approach of Fusion Glycoprotein (F) targeted multi-epitope vaccine against Human Respiratory Syncytial Virus (HRSV)
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2023.
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Brac University
2024
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| オンライン･アクセス: | http://hdl.handle.net/10361/22981 |
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10361-229812024-05-29T21:01:41Z In-silico approach of Fusion Glycoprotein (F) targeted multi-epitope vaccine against Human Respiratory Syncytial Virus (HRSV) Sejan, Al Saba Siam , Mohammad Kawsar Sharif School of Pharmacy, Brac University Respiratory syncytial virus Multi-epitope vaccine Fusion glycoprotein Biochemical analysis Immune simulation Respiratory syncytial virus. Respiratory infections. This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2023. Cataloged from PDF version of thesis. Includes bibliographical references (pages 50-55). "This work created a multi-epitope respiratory syncytial virus vaccine in-silico and simulated its biochemical effectiveness. In-silico investigation selected fusion glycoprotein (F) from envelope proteins of RSV. F protein causes virion-target cell membrane fusion. However, in-silico methodology used several servers, databases, and software such as Vaxijen v2.0 for antigenicity, NetCTL-1.2, NetMHCIIpan 4.0, and Bepipred servers, which were utilized to find epitopes recognized by cytotoxic T lymphocytes (CTLs), helper T lymphocytes (HTLs), and B cells. IFNepitope, IL-4Pred, and IL-10Pred were used to identify epitopes expressed by HTLs. Linkers were used to connect epitopes. Positive findings were found in biochemical analysis of the final proposed vaccine. Positive outcomes were predicted for a range of indicators, including instability index (38.99 as stable), GRAVY score (-0.180), molecular weight (91876.78 dalton), toxicity, antigenicity (0.5810), and allergenicity. Furthermore, acceptable z-score (-9.89) and Ramachandran plot (94.95%) were obtained for the final proposed vaccine via ProSAweb and SWISS-MODEL, respectively. The required outcome was also achieved by homology modeling, molecular docking, and immune response simulation via responsible servers. However, in-silico vaccine discovery for RSV and other diseases may be accelerated" Al Saba Sejan B. Pharmacy 2024-05-29T04:58:31Z 2024-05-29T04:58:31Z 2023 2023-02 Thesis ID 18346029 http://hdl.handle.net/10361/22981 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 55 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Respiratory syncytial virus Multi-epitope vaccine Fusion glycoprotein Biochemical analysis Immune simulation Respiratory syncytial virus. Respiratory infections. |
| spellingShingle |
Respiratory syncytial virus Multi-epitope vaccine Fusion glycoprotein Biochemical analysis Immune simulation Respiratory syncytial virus. Respiratory infections. Sejan, Al Saba In-silico approach of Fusion Glycoprotein (F) targeted multi-epitope vaccine against Human Respiratory Syncytial Virus (HRSV) |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2023. |
| author2 |
Siam , Mohammad Kawsar Sharif |
| author_facet |
Siam , Mohammad Kawsar Sharif Sejan, Al Saba |
| format |
Thesis |
| author |
Sejan, Al Saba |
| author_sort |
Sejan, Al Saba |
| title |
In-silico approach of Fusion Glycoprotein (F) targeted multi-epitope vaccine against Human Respiratory Syncytial Virus (HRSV) |
| title_short |
In-silico approach of Fusion Glycoprotein (F) targeted multi-epitope vaccine against Human Respiratory Syncytial Virus (HRSV) |
| title_full |
In-silico approach of Fusion Glycoprotein (F) targeted multi-epitope vaccine against Human Respiratory Syncytial Virus (HRSV) |
| title_fullStr |
In-silico approach of Fusion Glycoprotein (F) targeted multi-epitope vaccine against Human Respiratory Syncytial Virus (HRSV) |
| title_full_unstemmed |
In-silico approach of Fusion Glycoprotein (F) targeted multi-epitope vaccine against Human Respiratory Syncytial Virus (HRSV) |
| title_sort |
in-silico approach of fusion glycoprotein (f) targeted multi-epitope vaccine against human respiratory syncytial virus (hrsv) |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/22981 |
| work_keys_str_mv |
AT sejanalsaba insilicoapproachoffusionglycoproteinftargetedmultiepitopevaccineagainsthumanrespiratorysyncytialvirushrsv |
| _version_ |
1814307747688087552 |