Establishment of tissue culture protocol of Brassica Juncea var. BARI Sarisha-11
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024.
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Brac University
2024
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10361-229252024-07-14T05:53:13Z Establishment of tissue culture protocol of Brassica Juncea var. BARI Sarisha-11 Saha, Hridi Prova Jawad, Alisa Zaman, Samiha Nujhat Roy, Asmita Islam, Aparna Department of Mathematics and Natural Sciences, Brac University Tissue culture Sterilization Germination Rooting Plant tissue culture Germination Sterilization This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024. Catalogued from PDF version of thesis. Includes bibliographical references (pages 41-47). Brassica sp., commonly known as mustard, holds significant economic importance as a crop with high oil content, particularly in Bangladesh. Despite widespread demand, obstacles in production such as insufficient germination and various biotic and abiotic factors persist, biotechnological methods, including tissue culture, gene transfer, and genome editing systems, are increasingly employed to enhance this crop quality. This study focuses on improving Brassica juncea, specifically the variety BARI Sarisha-11, a vital mustard variety in Bangladesh. The primary objective is to refine a tissue culture-mediated regeneration procedure, laying the groundwork for future applications in micropropagation. In this study, to achieve high-frequency, uncontaminated germination, a seed sterilization protocol was established. Various combinations and durations of 70% ethanol, 0.1% mercury chloride, and 10% Clorox solution were tested. Among them, the highest optimal uncontaminated germination was obtained when seeds were sterilized with 70% ethanol for one minute and 0.1% mercury chloride for ten minutes. For shoot regeneration, 7 days old- cotyledonary leaves with petiole explants were used in nutrient media enriched with plant growth regulators, specifically 6-benzylaminopurine (BAP), 6-furfurylaminopurine (Kinetin/Kn), and naphthalene acetic acid (NAA). The highest regeneration (75%) was achieved with 1.0 mg/L BAP, 0.1 mg/L NAA, and 0.5 mg/L Kn. Rooting was examined using hormone less half-strength MS media, and acclimatization of plantlets was done in sterilized potted soil. Only one plantlet was produced following transplantation. In summary, given the crucial role of mustard as the primary oilseed crop in Bangladesh, this study represents a vital initial step in implementing mustard crop improvement techniques. The establishment of a practical, efficient, and replicable regeneration process, as demonstrated in this study, holds significant importance for the future of mustard cultivation. Hridi Prova Saha Alisa Jawad Samiha Nujhat Zaman Asmita Roy B. Biotechnology 2024-05-26T06:08:20Z 2024-05-26T06:08:20Z ©2024 2024-03 Thesis ID 20136050 ID 20136039 ID 20136033 ID 20136020 http://hdl.handle.net/10361/22925 en Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 48 pages application/pdf Brac University |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
Tissue culture Sterilization Germination Rooting Plant tissue culture Germination Sterilization |
| spellingShingle |
Tissue culture Sterilization Germination Rooting Plant tissue culture Germination Sterilization Saha, Hridi Prova Jawad, Alisa Zaman, Samiha Nujhat Roy, Asmita Establishment of tissue culture protocol of Brassica Juncea var. BARI Sarisha-11 |
| description |
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2024. |
| author2 |
Islam, Aparna |
| author_facet |
Islam, Aparna Saha, Hridi Prova Jawad, Alisa Zaman, Samiha Nujhat Roy, Asmita |
| format |
Thesis |
| author |
Saha, Hridi Prova Jawad, Alisa Zaman, Samiha Nujhat Roy, Asmita |
| author_sort |
Saha, Hridi Prova |
| title |
Establishment of tissue culture protocol of Brassica Juncea var. BARI Sarisha-11 |
| title_short |
Establishment of tissue culture protocol of Brassica Juncea var. BARI Sarisha-11 |
| title_full |
Establishment of tissue culture protocol of Brassica Juncea var. BARI Sarisha-11 |
| title_fullStr |
Establishment of tissue culture protocol of Brassica Juncea var. BARI Sarisha-11 |
| title_full_unstemmed |
Establishment of tissue culture protocol of Brassica Juncea var. BARI Sarisha-11 |
| title_sort |
establishment of tissue culture protocol of brassica juncea var. bari sarisha-11 |
| publisher |
Brac University |
| publishDate |
2024 |
| url |
http://hdl.handle.net/10361/22925 |
| work_keys_str_mv |
AT sahahridiprova establishmentoftissuecultureprotocolofbrassicajunceavarbarisarisha11 AT jawadalisa establishmentoftissuecultureprotocolofbrassicajunceavarbarisarisha11 AT zamansamihanujhat establishmentoftissuecultureprotocolofbrassicajunceavarbarisarisha11 AT royasmita establishmentoftissuecultureprotocolofbrassicajunceavarbarisarisha11 |
| _version_ |
1814309154519515136 |